diff --git a/crates/ruff_linter/resources/test/fixtures/pyupgrade/UP025.py b/crates/ruff_linter/resources/test/fixtures/pyupgrade/UP025.py
index 56a7484601111..d53c197a1f65a 100644
--- a/crates/ruff_linter/resources/test/fixtures/pyupgrade/UP025.py
+++ b/crates/ruff_linter/resources/test/fixtures/pyupgrade/UP025.py
@@ -25,3 +25,6 @@
 
 def hello():
     return"Hello"
+
+f"foo"u"bar"
+f"foo" u"bar"
diff --git a/crates/ruff_linter/src/checkers/ast/analyze/expression.rs b/crates/ruff_linter/src/checkers/ast/analyze/expression.rs
index 99f958684de32..5e26059969650 100644
--- a/crates/ruff_linter/src/checkers/ast/analyze/expression.rs
+++ b/crates/ruff_linter/src/checkers/ast/analyze/expression.rs
@@ -1266,9 +1266,6 @@ pub(crate) fn expression(expr: &Expr, checker: &mut Checker) {
             if checker.enabled(Rule::HardcodedTempFile) {
                 flake8_bandit::rules::hardcoded_tmp_directory(checker, string);
             }
-            if checker.enabled(Rule::UnicodeKindPrefix) {
-                pyupgrade::rules::unicode_kind_prefix(checker, string);
-            }
             if checker.source_type.is_stub() {
                 if checker.enabled(Rule::StringOrBytesTooLong) {
                     flake8_pyi::rules::string_or_bytes_too_long(checker, expr);
diff --git a/crates/ruff_linter/src/checkers/tokens.rs b/crates/ruff_linter/src/checkers/tokens.rs
index 9f3f0866f7e63..cfb9738691005 100644
--- a/crates/ruff_linter/src/checkers/tokens.rs
+++ b/crates/ruff_linter/src/checkers/tokens.rs
@@ -91,6 +91,10 @@ pub(crate) fn check_tokens(
         pycodestyle::rules::tab_indentation(&mut diagnostics, tokens, locator, indexer);
     }
 
+    if settings.rules.enabled(Rule::UnicodeKindPrefix) {
+        pyupgrade::rules::unicode_kind_prefix(&mut diagnostics, tokens);
+    }
+
     if settings.rules.any_enabled(&[
         Rule::InvalidCharacterBackspace,
         Rule::InvalidCharacterSub,
diff --git a/crates/ruff_linter/src/registry.rs b/crates/ruff_linter/src/registry.rs
index 2e6fcc199580e..1468db59aed3e 100644
--- a/crates/ruff_linter/src/registry.rs
+++ b/crates/ruff_linter/src/registry.rs
@@ -297,6 +297,7 @@ impl Rule {
             | Rule::TabIndentation
             | Rule::TrailingCommaOnBareTuple
             | Rule::TypeCommentInStub
+            | Rule::UnicodeKindPrefix
             | Rule::UselessSemicolon
             | Rule::UTF8EncodingDeclaration => LintSource::Tokens,
             Rule::IOError => LintSource::Io,
diff --git a/crates/ruff_linter/src/rules/pyupgrade/rules/unicode_kind_prefix.rs b/crates/ruff_linter/src/rules/pyupgrade/rules/unicode_kind_prefix.rs
index 13ecab830421d..481bd9548253c 100644
--- a/crates/ruff_linter/src/rules/pyupgrade/rules/unicode_kind_prefix.rs
+++ b/crates/ruff_linter/src/rules/pyupgrade/rules/unicode_kind_prefix.rs
@@ -1,9 +1,10 @@
 use ruff_diagnostics::{AlwaysFixableViolation, Diagnostic, Edit, Fix};
 use ruff_macros::{derive_message_formats, violation};
-use ruff_python_ast::ExprStringLiteral;
-use ruff_text_size::{Ranged, TextRange, TextSize};
 
-use crate::checkers::ast::Checker;
+use ruff_python_parser::lexer::LexResult;
+use ruff_python_parser::{StringKind, Tok};
+
+use ruff_text_size::{Ranged, TextRange, TextSize};
 
 /// ## What it does
 /// Checks for uses of the Unicode kind prefix (`u`) in strings.
@@ -39,13 +40,19 @@ impl AlwaysFixableViolation for UnicodeKindPrefix {
 }
 
 /// UP025
-pub(crate) fn unicode_kind_prefix(checker: &mut Checker, string: &ExprStringLiteral) {
-    if string.unicode {
-        let mut diagnostic = Diagnostic::new(UnicodeKindPrefix, string.range);
-        diagnostic.set_fix(Fix::safe_edit(Edit::range_deletion(TextRange::at(
-            string.start(),
-            TextSize::from(1),
-        ))));
-        checker.diagnostics.push(diagnostic);
+pub(crate) fn unicode_kind_prefix(diagnostics: &mut Vec<Diagnostic>, tokens: &[LexResult]) {
+    for (token, range) in tokens.iter().flatten() {
+        if let Tok::String {
+            kind: StringKind::Unicode,
+            ..
+        } = token
+        {
+            let mut diagnostic = Diagnostic::new(UnicodeKindPrefix, *range);
+            diagnostic.set_fix(Fix::safe_edit(Edit::range_deletion(TextRange::at(
+                range.start(),
+                TextSize::from(1),
+            ))));
+            diagnostics.push(diagnostic);
+        }
     }
 }
diff --git a/crates/ruff_linter/src/rules/pyupgrade/snapshots/ruff_linter__rules__pyupgrade__tests__UP025.py.snap b/crates/ruff_linter/src/rules/pyupgrade/snapshots/ruff_linter__rules__pyupgrade__tests__UP025.py.snap
index efa31f73cda2f..256e6222ec329 100644
--- a/crates/ruff_linter/src/rules/pyupgrade/snapshots/ruff_linter__rules__pyupgrade__tests__UP025.py.snap
+++ b/crates/ruff_linter/src/rules/pyupgrade/snapshots/ruff_linter__rules__pyupgrade__tests__UP025.py.snap
@@ -248,4 +248,37 @@ UP025.py:19:5: UP025 [*] Remove unicode literals from strings
 21 21 | # These should not change
 22 22 | u = "Hello"
 
+UP025.py:29:7: UP025 [*] Remove unicode literals from strings
+   |
+27 |     return"Hello"
+28 | 
+29 | f"foo"u"bar"
+   |       ^^^^^^ UP025
+30 | f"foo" u"bar"
+   |
+   = help: Remove unicode prefix
+
+ℹ Safe fix
+26 26 | def hello():
+27 27 |     return"Hello"
+28 28 | 
+29    |-f"foo"u"bar"
+   29 |+f"foo""bar"
+30 30 | f"foo" u"bar"
+
+UP025.py:30:8: UP025 [*] Remove unicode literals from strings
+   |
+29 | f"foo"u"bar"
+30 | f"foo" u"bar"
+   |        ^^^^^^ UP025
+   |
+   = help: Remove unicode prefix
+
+ℹ Safe fix
+27 27 |     return"Hello"
+28 28 | 
+29 29 | f"foo"u"bar"
+30    |-f"foo" u"bar"
+   30 |+f"foo" "bar"
+