diff --git a/source/website/src/views/UploadToAWSS3.vue b/source/website/src/views/UploadToAWSS3.vue
index 379c07f..9d21c7e 100755
--- a/source/website/src/views/UploadToAWSS3.vue
+++ b/source/website/src/views/UploadToAWSS3.vue
@@ -759,12 +759,14 @@ export default {
       const TransformText = {
         WebToSRTCaptions: {
           MediaType: "MetadataOnly",
-          TargetLanguageCodes: Object.values(this.selectedTranslateLanguages.map(x => x.text)).filter(x => x !== this.sourceLanguageCode).concat(this.sourceLanguageCode),
+          // Exclude 'auto' from target languages when user specified 'auto' as the source language
+          TargetLanguageCodes: Object.values(this.selectedTranslateLanguages.map(x => x.text)).filter(x => x !== this.sourceLanguageCode).concat(this.sourceLanguageCode).filter(x => x !== 'auto'),
           Enabled: this.enabledOperators.includes("Transcribe") || this.enabledOperators.includes("Translate")
         },
         WebToVTTCaptions: {
           MediaType: "MetadataOnly",
-          TargetLanguageCodes: Object.values(this.selectedTranslateLanguages.map(x => x.text)).filter(x => x !== this.sourceLanguageCode).concat(this.sourceLanguageCode),
+          // Exclude 'auto' from target languages when user specified 'auto' as the source language
+          TargetLanguageCodes: Object.values(this.selectedTranslateLanguages.map(x => x.text)).filter(x => x !== this.sourceLanguageCode).concat(this.sourceLanguageCode).filter(x => x !== 'auto'),
           Enabled: this.enabledOperators.includes("Transcribe") || this.enabledOperators.includes("Translate")
         },
         PollyWebCaptions: {