diff --git a/README.md b/README.md index 67a2939..24dc99d 100644 --- a/README.md +++ b/README.md @@ -6,6 +6,9 @@ The second version of NanoSim (v2.0) uses minimap2 as default aligner to align l __Citation__: Chen Yang, Justin Chu, René L Warren, Inanç Birol; NanoSim: nanopore sequence read simulator based on statistical characterization. Gigascience 2017 gix010. doi: 10.1093/gigascience/gix010 +## Transcriptome simulation +For transcriptome simulaton (directRNA / cDNA reads), please use [Trans-NanoSim](https://github.com/bcgsc/Trans-NanoSim) for now. We are working on merging Trans-NanoSim pipeline into NanoSim repository and it will be available shortly. + ## Dependencies minimap2 (Tested with version 2.10) LAST (Tested with version 581 and 916) @@ -107,5 +110,6 @@ _See more detailed example in example.sh_ 3. `simulated_error_profile` Contains all the information of errors introduced into each reads, including error type, position, original bases and current bases. + ## Acknowledgements Sincere thanks to my labmates and all contributors and users to this tool.