3Dscript is a plugin for Fiji/ImageJ for creating 3D and 4D animations of microscope data. In contrast to existing 3D visualization packages, animations are not keyframe-based, but are described by a natural language-based syntax.
Find more information at https://bene51.github.io/3Dscript
- 64-bit Operating System (Linux, Mac OS X or Windows)
- Up-to-date Fiji installation (http://www.fiji.sc)
- OpenCL 1.2-capable Graphics Card or better
- Decent graphics card, preferably from Nvidia or AMD, but newer Intel cards will work also (see below for tested hardware)
- Start Fiji
- Click on Help>Update...
- Click on "Manage update sites"
- Check the box in front of "3Dscript"
- Click on "Close"
- Click on "Apply changes"
- Restart Fiji
- Installation typically takes not longer than a couple of minutes
- Start Fiji
- Click on File>Open Samples>T1 Head
- Click on Plugins>3D script>Interactive Animation
- In the "Interactive Raycaster" window, click on "show" next to "Animation"
- Click on "Start text-based animation editor"
- In the editor window, type the following text: From frame 0 to frame 200 rotate by 360 degrees horizontally
- Click on "Run" This will render 200 frames of a movie sequence, within which the MRI data set rotates by 360 degrees.
Rendering of 200 frames of this data set will typically take less than a minute on an OpenCL-enabled Graphics Card. The resulting stack can be saved as a video file using Fiji's File>Save As>AVI... command.
To run the software on another data set, open a different image stack (instead of the T1 Head sample data) before running 3Dscript.
More information is available at https://bene51.github.io/3Dscript
Detailed usage information is available on the wiki.
Schmid, B.; Tripal, P. & Fraaß, T. et al. (2019), "3Dscript: animating 3D/4D microscopy data using a natural-language-based syntax", Nature methods 16(4): 278–280, PMID 30886414.
|Graphics card||Operating system|
|NVIDIA Quadro P2000||Windows 10|
|NVIDIA Quadro M4000||Windows 10|
|NVIDIA Quadro M2000||Windows 10|
|NVIDIA Quadro K620||Windows 10|
|NVIDIA Quadro K600||Windows 10|
|NVIDIA Quadro K420||Windows 7|
|NVIDIA GeForce GTX760||Windows 7|
|AMD FirePro W5100||Windows 7|
|Intel HD Graphics 4600||Windows 7|
|Intel HD Graphics 4000||Mac OS X|
|Intel HD Graphics 5000||Mac OS X|
|Intel HD Graphics 530||Windows 10|