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Is it possible to use DADA2 to process whole 16S single read fastq files generated by Nanoporetech MinION? The reads are on average 1500nt in length, and forward and reverse reads are interleaved in the same file. Thanks for your input.
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Nope, not sensibly anyway. Nanopore error rates are way too high to meet DADA2's assumption that at least some complete error-free reads exist in the data.
Is it possible to use DADA2 to process whole 16S single read fastq files generated by Nanoporetech MinION? The reads are on average 1500nt in length, and forward and reverse reads are interleaved in the same file. Thanks for your input.
The text was updated successfully, but these errors were encountered: