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Automating Aquatic Respirometry. Focused on automating intermittent respirometry experiments for aquatic animals.

Now compatible with the new firmware from PyroScience - those using WorkBench See and use OxyLog

The GUI and code controlling of the experiment, and the code calculating the results are licensed all licensed under GPL, any figures etc under Creative Commons BY-SA.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Please cite the code when using the software


Cite as: Morten Bo Søndergaard Svendsen, Peter G. Bushnell, & John Fleng Steffensen. (2019, March 5). AquaResp 3 (Version V3.0). Zenodo.

Oxygen meter communication

The fundamental idea is that external software logs to a the oxygen folder, where Aquaresp then can read the data.

I have made a quick guide explaining usage of primarily PyroScience meters in AquaResp. The approach for Firesting firmware <4, is the same for fibox - presens.


Online slides


AquaResp is only supported on Windows 10 64 bit.

Illustrated process SLIDES

PDF - Installation Illustrated

Download Aquaresp by pressing "Clone or Download" button in the top right corner of this screen.

Windows 10 - 64bit:

Step 1 - downloading Python

First run 1.vbs in the Installation folder

This downloads Python for you, the version that has been used for testing (3.7.4. - 64-bit)

Step 2 - installing Python and libraries

Run 2.bat

This initiates Python installation for you.

If this fails, open the downloaded Python (InstallPython.exe), and make sure to enable the option "Add to PATH" during the first step of installation

After Python has been installed

Double click the in the installation folder.

Click each button, this will check and install the libraries needed to run AquaResp 3.0 ASAP The buttons will change to green and display "OK" when libraries are installed.

####Further: We recommend setting either Mozilla Firefox or Google Chrome webbrowsers as default browser in Windows. The plotting feature does not work in Internet Explorer or Microsoft Edge browsers.

March 5 2021: If you are experiencing trouble with running the software after installation. Try running the tool in the Installation folder.

October 2021: For newer win10 updates, run the CheckLibraries as administrator

November 2021: Version 3.01. Compatibility with PyroScience WorkBench.

If this step is failing, open the command prompt (WinButton + R, write "cmd", and press enter), and run the installation commands for the specific library. The following are needed for AQ3

python -m pip install bokeh==1.3.0

python -m pip install numpy

python -m pip install scipy

python -m pip install matplotlib

python -m pip install -U wxPython

python -m pip install mcculw


Step 3 - adding Icons to your desktop

Run Create Links on Desktop.vbs (in the Aquaresp 3 main folder)

This creates icons on the desktop. You can do that manually aswell.


Thank you to Bent Vismann for testing of AquaResp and inputs on usage of AquaResp for invertebrates.

Thank you to Denis Chabot for testing of AquaResp over many occasions and providing inputs on usage of AquaResp.

Thank you to Heiðrikur Bergsson for testing and using AquaResp.

Thank you to Emil Aputsiaq Christensen for providing valuable usability input.

Thank you to Lars Emil Juel Andersen for being exeptionally fast in trying new AquaResp versions and letting me know of any bugs.


Automating Aquatic Respirometry. Focused on automating intermittent respirometry experiments for aquatic animals.







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