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Tidy up FreeBayes #67

fnothaft opened this Issue Aug 31, 2017 · 4 comments


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fnothaft commented Aug 31, 2017

  • bump to
  • mount reference file into docker container
  • support copying files to executor
  • run in -i mode
  • specify -region when invoking FreeBayes not necessary
  • VCF comes out mis-ordered
  • use Avocado read/contig filtering mechanism (bigdatagenomics/avocado#254)

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heuermh commented Sep 7, 2017

Can we include test resources from here in cli/src/test/resources? MIT License but the test data don't have a copyright notice to include.

@heuermh heuermh modified the milestones: 0.1.0, 0.2.0 Jan 24, 2018


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heuermh commented Feb 14, 2018

With the fix for bigdatagenomics/avocado#254 (commit bigdatagenomics/avocado@5f720cf), do you intend that Cannoli would depend on Avocado?


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heuermh commented Feb 27, 2018

Taking a closer look at the freebayes command line arguments, we should always use

                   Generate strict VCF format (FORMAT/GQ will be an int)

and should provide arguments in the Freebayes wrapper for

   -A --cnv-map FILE
                   Read a copy number map from the BED file FILE, which has
                   the format:
                      reference sequence, start, end, sample name, copy number
                   ... for each region in each sample which does not have the
                   default copy number as set by --ploidy.

                   Write gVCF output, which indicates coverage in uncalled regions.

   --gvcf-chunk NUM
                   When writing gVCF output emit a record for every NUM bases.

As to the rest, I'm not sure if we should wrap all of them, or provide a bulk argument/config mechanism, or simply say if you need to provide non-default parameters, call pipe manually.


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heuermh commented Mar 22, 2018

Fixed by #107.

@heuermh heuermh closed this Mar 22, 2018

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