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Import Bio::SeqIO abi, alf, ctf, exp, pln, and ztr
Imported from bioperl-live c98b3b5ccff668d55a05bf9516f172a1a5be946c Only adapted the tests. The test data files in bioperl-live are the same data files already in this repo, only had a different name. The tests for alf.t try to make use of a test.alf that didn't exist in bioperl-live and also does not exist here in bioperl-ext.
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# BioPerl module for Bio::SeqIO::abi | ||
# | ||
# Please direct questions and support issues to <bioperl-l@bioperl.org> | ||
# | ||
# Cared for by Aaron Mackey <amackey@virginia.edu> | ||
# | ||
# Copyright Aaron Mackey | ||
# | ||
# You may distribute this module under the same terms as perl itself | ||
|
||
# POD documentation - main docs before the code | ||
|
||
=head1 NAME | ||
Bio::SeqIO::abi - abi trace sequence input/output stream | ||
=head1 SYNOPSIS | ||
Do not use this module directly. Use it via the Bio::SeqIO class. | ||
=head1 DESCRIPTION | ||
This object can transform Bio::Seq objects to and from abi trace | ||
files. To optionally read the trace graph data (which can be used | ||
to draw chromatographs, for instance), set the optional | ||
'-get_trace_data' flag or the get_trace_data method to a value | ||
evaluating to TRUE. | ||
=head1 FEEDBACK | ||
=head2 Mailing Lists | ||
User feedback is an integral part of the evolution of this and other | ||
Bioperl modules. Send your comments and suggestions preferably to one | ||
of the Bioperl mailing lists. Your participation is much appreciated. | ||
bioperl-l@bioperl.org - General discussion | ||
http://bioperl.org/wiki/Mailing_lists - About the mailing lists | ||
=head2 Support | ||
Please direct usage questions or support issues to the mailing list: | ||
I<bioperl-l@bioperl.org> | ||
rather than to the module maintainer directly. Many experienced and | ||
reponsive experts will be able look at the problem and quickly | ||
address it. Please include a thorough description of the problem | ||
with code and data examples if at all possible. | ||
=head2 Reporting Bugs | ||
Report bugs to the Bioperl bug tracking system to help us keep track | ||
the bugs and their resolution. | ||
Bug reports can be submitted via the web: | ||
https://github.com/bioperl/bioperl-live/issues | ||
=head1 AUTHORS - Aaron Mackey | ||
Email: amackey@virginia.edu | ||
=head1 APPENDIX | ||
The rest of the documentation details each of the object | ||
methods. Internal methods are usually preceded with a _ | ||
=cut | ||
|
||
# Let the code begin... | ||
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package Bio::SeqIO::abi; | ||
use vars qw(@ISA $READ_AVAIL); | ||
use strict; | ||
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use Bio::SeqIO; | ||
use Bio::Seq::SeqFactory; | ||
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push @ISA, qw( Bio::SeqIO ); | ||
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sub BEGIN { | ||
eval { require Bio::SeqIO::staden::read; }; | ||
if ($@) { | ||
$READ_AVAIL = 0; | ||
} else { | ||
push @ISA, "Bio::SeqIO::staden::read"; | ||
$READ_AVAIL = 1; | ||
} | ||
} | ||
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sub _initialize { | ||
my($self,@args) = @_; | ||
$self->SUPER::_initialize(@args); | ||
my ($get_trace) = $self->_rearrange([qw(get_trace_data)],@args); | ||
$get_trace && $self->get_trace_data(1); | ||
if( ! defined $self->sequence_factory ) { | ||
$self->sequence_factory(Bio::Seq::SeqFactory->new(-verbose => $self->verbose(), -type => 'Bio::Seq::Quality')); | ||
} | ||
unless ($READ_AVAIL) { | ||
Bio::Root::Root->throw( -class => 'Bio::Root::SystemException', | ||
-text => "Bio::SeqIO::staden::read is not available; make sure the bioperl-ext package has been installed successfully!" | ||
); | ||
} | ||
} | ||
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=head2 next_seq | ||
Title : next_seq | ||
Usage : $seq = $stream->next_seq() | ||
Function: returns the next sequence in the stream | ||
Returns : Bio::Seq::Quality object | ||
Args : NONE | ||
=cut | ||
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sub next_seq { | ||
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my ($self) = @_; | ||
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my ($seq, $id, $desc, $qual) = $self->read_trace($self->_fh, 'abi'); | ||
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# create the seq object | ||
my ($base_locs, $a_trace, $c_trace, $g_trace, $t_trace, $points, $max_height); | ||
if ($self->get_trace_data) { | ||
($base_locs, $a_trace, $c_trace, $g_trace, $t_trace, $points, $max_height) = $self->read_trace_with_graph($self->_fh, 'abi'); | ||
} else { | ||
$base_locs = []; | ||
} | ||
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# create the seq object | ||
$seq = $self->sequence_factory->create(-seq => $seq, | ||
-id => $id, | ||
-primary_id => $id, | ||
-desc => $desc, | ||
-alphabet => 'DNA', | ||
-qual => $qual, | ||
-trace => join (" ", @{$base_locs}), | ||
-trace_data => { a_trace => $a_trace, | ||
c_trace => $c_trace, | ||
g_trace => $g_trace, | ||
t_trace => $t_trace, | ||
max_height => $max_height, | ||
num_points => $points } | ||
); | ||
return $seq; | ||
} | ||
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=head2 write_seq | ||
Title : write_seq | ||
Usage : $stream->write_seq(@seq) | ||
Function: writes the $seq object into the stream | ||
Returns : 1 for success and 0 for error | ||
Args : Bio::Seq object | ||
=cut | ||
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sub write_seq { | ||
my ($self,@seq) = @_; | ||
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my $fh = $self->_fh; | ||
foreach my $seq (@seq) { | ||
$self->write_trace($fh, $seq, 'abi'); | ||
} | ||
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$self->flush if $self->_flush_on_write && defined $self->_fh; | ||
return 1; | ||
} | ||
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=head2 get_trace_data | ||
Title : get_trace_data | ||
Usage : $stream->get_trace_data(1) | ||
Function: set boolean flag to retrieve the trace data (possibly for | ||
output) | ||
Returns : bool value, TRUE = retrieve trace data (default FALSE) | ||
Args : bool value | ||
=cut | ||
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sub get_trace_data { | ||
my ($self, $val) = @_; | ||
$self->{_get_trace_data} = $val ? 1 : 0 if (defined $val); | ||
$self->{_get_trace_data}; | ||
} | ||
|
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1; |
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# BioPerl module for Bio::SeqIO::alf | ||
# | ||
# Please direct questions and support issues to <bioperl-l@bioperl.org> | ||
# | ||
# Cared for by Aaron Mackey <amackey@virginia.edu> | ||
# | ||
# Copyright Aaron Mackey | ||
# | ||
# You may distribute this module under the same terms as perl itself | ||
|
||
# POD documentation - main docs before the code | ||
|
||
=head1 NAME | ||
Bio::SeqIO::alf - alf trace sequence input/output stream | ||
=head1 SYNOPSIS | ||
Do not use this module directly. Use it via the Bio::SeqIO class. | ||
=head1 DESCRIPTION | ||
This object can transform Bio::Seq objects to and from alf trace | ||
files. | ||
=head1 FEEDBACK | ||
=head2 Mailing Lists | ||
User feedback is an integral part of the evolution of this and other | ||
Bioperl modules. Send your comments and suggestions preferably to one | ||
of the Bioperl mailing lists. Your participation is much appreciated. | ||
bioperl-l@bioperl.org - General discussion | ||
http://bioperl.org/wiki/Mailing_lists - About the mailing lists | ||
=head2 Support | ||
Please direct usage questions or support issues to the mailing list: | ||
I<bioperl-l@bioperl.org> | ||
rather than to the module maintainer directly. Many experienced and | ||
reponsive experts will be able look at the problem and quickly | ||
address it. Please include a thorough description of the problem | ||
with code and data examples if at all possible. | ||
=head2 Reporting Bugs | ||
Report bugs to the Bioperl bug tracking system to help us keep track | ||
the bugs and their resolution. | ||
Bug reports can be submitted via the web: | ||
https://github.com/bioperl/bioperl-live/issues | ||
=head1 AUTHORS - Aaron Mackey | ||
Email: amackey@virginia.edu | ||
=head1 APPENDIX | ||
The rest of the documentation details each of the object | ||
methods. Internal methods are usually preceded with a _ | ||
=cut | ||
|
||
# Let the code begin... | ||
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package Bio::SeqIO::alf; | ||
use vars qw(@ISA $READ_AVAIL); | ||
use strict; | ||
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use Bio::SeqIO; | ||
use Bio::Seq::SeqFactory; | ||
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push @ISA, qw( Bio::SeqIO ); | ||
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sub BEGIN { | ||
eval { require Bio::SeqIO::staden::read; }; | ||
if ($@) { | ||
$READ_AVAIL = 0; | ||
} else { | ||
push @ISA, "Bio::SeqIO::staden::read"; | ||
$READ_AVAIL = 1; | ||
} | ||
} | ||
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sub _initialize { | ||
my($self,@args) = @_; | ||
$self->SUPER::_initialize(@args); | ||
if( ! defined $self->sequence_factory ) { | ||
$self->sequence_factory(Bio::Seq::SeqFactory->new(-verbose => $self->verbose(), -type => 'Bio::Seq::Quality')); | ||
} | ||
unless ($READ_AVAIL) { | ||
Bio::Root::Root->throw( -class => 'Bio::Root::SystemException', | ||
-text => "Bio::SeqIO::staden::read is not available; make sure the bioperl-ext package has been installed successfully!" | ||
); | ||
} | ||
} | ||
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=head2 next_seq | ||
Title : next_seq | ||
Usage : $seq = $stream->next_seq() | ||
Function: returns the next sequence in the stream | ||
Returns : Bio::Seq::Quality object | ||
Args : NONE | ||
=cut | ||
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sub next_seq { | ||
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my ($self) = @_; | ||
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my ($seq, $id, $desc, $qual) = $self->read_trace($self->_fh, 'alf'); | ||
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# create the seq object | ||
$seq = $self->sequence_factory->create(-seq => $seq, | ||
-id => $id, | ||
-primary_id => $id, | ||
-desc => $desc, | ||
-alphabet => 'DNA', | ||
-qual => $qual | ||
); | ||
return $seq; | ||
} | ||
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=head2 write_seq | ||
Title : write_seq | ||
Usage : $stream->write_seq(@seq) | ||
Function: writes the $seq object into the stream | ||
Returns : 1 for success and 0 for error | ||
Args : Bio::Seq object | ||
=cut | ||
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sub write_seq { | ||
my ($self,@seq) = @_; | ||
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my $fh = $self->_fh; | ||
foreach my $seq (@seq) { | ||
$self->write_trace($fh, $seq, 'alf'); | ||
} | ||
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$self->flush if $self->_flush_on_write && defined $self->_fh; | ||
return 1; | ||
} | ||
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1; |
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