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Revision history for BioPerl core modules
The comprehensive history and ongoing development of BioPerl:
Some of that history is also highlighted on our wiki:
Bugs and requested features list:
CPAN releases are branched from 'master'.
[New features]
* Bio::Seq::SimulatedRead
- New module to represent reads taken from other sequences [fangly]
* Bio::Root::Root
- Support of Clone::Fast as a faster cloning alternative [fangly]
* Bio::Root::IO
- Moved the format() and variant() methods from Bio::*IO modules to
Bio::Root::IO [fangly]
- Can now use format() to get the type of IO format in use [fangly]
* Bio::Tools::IUPAC
- New regexp() method to create regular expressions from IUPAC sequences
* Bio::SeqFeature::Primer and Bio::Seq::PrimedSeq:
- Code refresh [fangly]
* Bio::DB::Taxonomy
- Added support for the Greengenes and Silva taxonomies [fangly]
* Bio::Tree::TreeFunctionsI
- get_lineage_string() represents a lineage as a string [fangly]
- add_trait() returns instead of reporting an error when the column
number is exceeded in add_trait() [fangly]
- Option to support tree leaves without trait [fangly]
- Allow ID of 0 in trait files [fangly]
* Bio::DB::Taxonomy::list
- Misc optimizations [fangly]
- Option -names of get_taxon() to help with ambiguous taxa [fangly]
* Bio::DB::Taxonomy::*
- get_num_taxa() returns the number of taxa in the database [fangly]
* Bio::DB::Fasta and Bio::DB::Qual
- support indexing an arbitrary list of files [fangly]
- user can supply an arbitrary index file name [fangly]
- new option to remove index file at the end [fangly]
* Bio::DB::Fasta
- now handles IUPAC degenerate residues [fangly]
[Bug fixes]
* [3240] Bio::AlignIO::stockholm now parses simple sequences [Bernd Web,
* [3237] Bio::DB::Fasta now allows blank lines between sequences, catches
instances where blank lines are within sequences [cjfields]
* Bio::DB::Fasta reports correct alphabet for files with multiple sequence
types [fangly]
* Bio::DB::Fasta rev-comps sequences other than DNA properly [fangly]
* [3238] Fixes for Bio::DB::SeqFeature::Store::DBI::Pg [Thomas Burkhard,
* Various fixes for Stockholm file indexing and processing [bosborne]
* Fix edge case in FASTQ parsing where sequence of length 1 and qual of 0
breaks parsing [cjfields]
* Fix case where Bio::Seq::Meta* objects with no meta information could not
be reverse-complemented [fangly]
* Fix bug for fields without aliases in Bio::DB::Query::HIVQuery [fangly]
* Fix Bio::PopGen::IO::phase: sort values lexically instead of numerically
when unsure that values will be numerical [fangly]
* Fix undef warnings in Bio::SeqIO::embl [fangly]
* Fix undef warnings in Bio::DB::Fasta and Bio::DB::Qual [fangly]
* Fix Bio::Tools::IUPAC should accept any sequence object [fangly]
* Fix for 'Inappropriate ioctl' in Bio::DB::Store::berkeleydb3 [Olivier
* Bio::SeqFeature::Generic SeqfeatureI compliance: methods primary_tag,
source_tag and display_name must return a string, not undef [fangly]
* Bio::SimpleAlign and Bio::Seq compliance with Bio::FeatureHolderI
add_SeqFeature takes a single argument [fangly]
* Use cross-platform filenames and temporary directory in
Bio::DB::Taxonomy::flatfile [fangly]
* Fix bug in Bio::DB::Taxonomy::list where taxa with no ancestors were not
properly identified as existing taxa in the database [fangly]
* Fix issue where a Bio::DB::Taxonomy::list object could not be created
without also passing a lineage to store [fangly]
* Prevent passing a directory to the gi2taxid option (-g) of and remove an 'earlier declaration' warning
* Fixed crash when missing source feature date [fangly]
1.6.901 May 18, 2011
* Use of AcePerl is deprecated; isn't actively maintained, and
modules using Ace will also be deprecated [lds, cjfields]
* Minor bug fix release
* Bio::SeqIO::gbxml tests require XML::SAX [hartzell]
* Address Build.PL issues when DBI is not present [hartzell]
* Skip gbxml.t and Interpro tests when modules not installed [cjfields]
* Remove deprecated code for perl 5.14.0 compat [cjfields]
* Due to schema changes and lack of support for older versions, support
for NeXML 0.9 is only (very) partially implemented.
[Bug fixes]
* [3205] - small fix to Bio::Perl blast_sequence() to make compliant with
docs [genehack, cjfields]
* $VERSION for CPAN/cpanm-based installs was broken; force setting of
module version from dist_version (probably not the best way to do this,
but it seems to work) [rbuels, cjfields]
1.6.900 April 14, 201
* This will probably be the last release to add significant features to
core modules; subsequent releases will be for bug fixes alone.
We are planning on a restructuring of core for summer 2011, potentially
as part of the Google Summer of Code. This may become BioPerl 2.0.
* Version bump represents 'just prior to v 1.7'. We may have point
releases to deal with bugs, with increments of 1.6.901, 1.6.902, etc.
This code essentially is what is on the github master branch.
[New features]
* Core code updated for perl 5.12.x [cjfields, Charle Tilford]
* Bio::Tree refactor
- major overhaul of Bio::Tree code by Greg Jordan, fixes several bugs
- removal of Scalar::Util::weaken code, which was causing odd headaches
with premature GC, memory leaks with perl 5.10.0, etc [cjfields]
* Bio::DB::SeqFeature bug fixes for GBrowse2 compatibility [lds, scottcain,
many others]
* Bio::SeqIO::msout, Bio::SeqIO::mbsout - parsers for ms and mbs
[Warren Kretzschmar]
* Bio::SeqIO::gbxml
- bug 2515 - new contribution [Ryan Golhar, jhannah]
* Bio::Assembly::IO
- support for reading Maq, Sam and Bowtie files [maj]
- support for reading 454 GS Assembler (Newbler) ACE files [fangly]
- bug 2483: support for writing ACE files [Joshua Udall, fangly]
- bug 2599: support DBLINK annotation in GenBank files [cjfields]
- bug 2726: reading/writing granularity: whole scaffold or one contig
at a time [Joshua Udall, fangly]
* Bio::OntologyIO
- Added parsing of xrefs to OBO files, which are stored as secondary
dbxrefs of the cvterm [Naama Menda]
- General Interpro-related code refactors [dukeleto, rbuels, cjfields]
* PAML code updated to work with PAML 4.4d [DaveMessina]
[Bug fixes]
* [3198] - sort tabular BLAST hits by score [DaveMessina]
* [3196] - fix invalid metadata produced by latest Module::Build [cjfields]
* [3190] - RemoteBlast GAPCOSTS regex fix [Ali Walsh, cjfields]
* [3185] - Bio::Tools::SeqStats->get_mol_wt now gives correct MW
* [3178] - fix tr/// issue in Bio::Range [Andrew Conley, cjfields]
* [3172] - Bio::DB::Fasta - catch possibly bad FASTA files [cjfields]
* [3164] - TreeFunctionsI syntax bug [gjuggler]
* [3163] - AssemblyIO speedup [fangly]
* [3160] - Bio::SearchIO::Writer::TextResultWriter output [Paul Cantalupo,
* [3159] - add SwissPfam support to bp_index.PLS [hyphaltip]
* [3158] - fix EMBL file mis-parsing [cjfields]
* [3157] - Bio::Restriction::Analysis 'sizes' method fixed [Marc Perry,
* [3153] - fix SeqIO::swiss TagTree issues [Charles Tilford, cjfields]
* [3148] - URL change for UniProt [cjfields]
* [3145] - AXT off-by-1 error [Aaron Goodman, cjfields]
* [3136] - HMMer3 parser fixes [kblin]
* [3126] - catch description [Toshihiko Akiba]
* [3122] - Catch instances where non-seekable filehandles were being
seek'd w/o checking for status [Stefan Kirov, Roy Chaudhuri]
* [3121] - Bio::OntologyIO cannot parse the full InterPro XML file
[dukeleto, rbuels, cjfields]
* [3120] - round-trip fixes [genehack, David Breimann,
* [3116,3117] - perl 5.12.x warnings fixed [cjfields, Charles Tilford]
* [3110] - Better 'namespace' support for bp_seqfeature_load.PLS [dbolser,
* [3107] - BLAST alignment column_from_residue_number() [cjfields]
* [3104] - Bio::Species single node hierarchies [Charles Tilford, cjfields]
* [3092, 3090] - parsing of BLAST HSP stats [Razi Khaja, cjfields]
* [3089] - HSPTableWriter missing methods [Robson de Souza, cjfields]
* [3086] - EMBL misparsing long tags [kblin, cjfields]
* [3085] - CommandExts and array of files [maj, hyphaltip]
* [3077] - Bio::SimpleAlign slice() now correctly computes seq coordinates
for alignment slices [Ha X. Dang, cjfields]
* [3076] - XMFA alignment strand wrong [Ha X., cjfields]
* [3073] - fix parsing of GenBank files from RDP [cjfields]
* [3068] - FASTQ parse failure with trailing 0 [cjfields]
* [3064] - All-gap midline BLAST report issues [cjfields]
* [3063] - BLASt report RID [Razi Khaja, cjfields]
* [3058] - SearchIO::fasta parsing [DaveMessina, cjfields]
* [3053] - LOCUS line formatting [M. Wayne, cjfields]
* [3039] - correct Newick output root node branch length [gjuggler,
* [3038] - SELEX alignment error [Bernd, cjfields]
* [3033] - PrimarySeq ID setting [Bernd, maj]
* [3032] - Fgenesh errors [Wes Barris, hyphaltip]
* [3034] - AlignIO::clustal output [Bernd, DaveMessina]
* [3031] - Parse algorithm ref for BLAST [Razi Khaja, cjfields]
* [3028] - Bio::TreeIO::nexus and FigTree compat [Kevin Balbi, cjfields]
* [3025] - Bio::SeqIO::embl infinite loop [Adam Sjøgren, cjfields]
* [3040, 3023, 2974, 2921, 2753, 2636, 2482] - PAML parser fixed, works with
PAML 4.4d [DaveMessina]
* [3015, 3022] - Bio::Restriction withrefm regexp [Emmanuel Quevillon,
* [3020] - GFF3Loader alias attribute [Nathan Weeks, cjfields]
* [3018, 3019, 3021] - gmap_f9 parsing [Kiran Mukhyala, cjfields]
* [3017] - using threads with Bio::DB::GenBank [cjfields]
* [3012] - Bio::Root::HTTPget fixes [maj, cjfields]
* [3011] - namespace support for SF::Store::DBI::Pg [Adam Witney, cjfields]
* [3002] - Bio::DB::EUtilities NCBI policy updates [cjfields]
* [3001] - seq identifier '0' dropped with FASTA [Michael Kuhn, maj]
* [2984] - let LocatableSeq decide on length of phylip aln [Adam Witney,
* [2983] - fix score/percent ID mixup [Alexie Papanicolaou]
* [2977] - TreeIO issues [DaveMessina]
* [2959] - Bio::SeqUtils->revcom_with_features [Roy Chaudhuri, maj]
* [2944] - Bio::Tools::GFF score [cjfields]
* [2942] - correct MapTiling output [maj]
* [2939] - PDB residue insertion codes [John May, maj]
* [2930] - PrimarySeqI term symbol [Adam Sjøgren, maj]
* [2928] - GuessSeqFormat raw [maj]
* [2926] - Bio:Tools::TandemRepeatsFinder seq_id [takadonet, cjfields]
* [2922] - open() directive issue [cjfields]
* [2915] - GenBank parser infinite loop [Francisco Ossandon, cjfields]
* [2901] - DNAStatistics div by zero error [Janet Young, cjfields]
* [2899] - SeqFeature::Store host issues [lstein, dbolser]
* [2897] - Add a "mask_below_threshold" method to Seq::Quality [dbolser,
* [2881] - .scf files don't' roundtrip [Adam Sjøgren, cjfields]
* [2876] - CDD search with RemoteBlast [Malcolm Cook]
* [2863] - Root::IO::_initialize_io causes crash [rbuels, maj, DaveMessina]
* [2845] - Bio::Seq::Quality gives seq with no ID [Tristan Lefebure, cjfields]
* [2843] - FeatureIO BED to GFF fails w/ no phase [cassjm cjfields]
* [2773] - Bio::Tree::Node premature GC [Morgan Price, cjfields]
* [2764] - add ID Tracker helper for SwissProt [heikki, cjfields]
* [2758] - Bio::AssemblyIO ace problems [fangly]
* [2744] - Bio::LocatableSeq::end [Bernd, cjfields]
* [2726] - ace file IO [Josh, fangly]
* [2700] - Refactor Build.PL [cjfields]
* [2673] - addition of simple Root-based clone() method [cjfields]
* [2648] - Bio::Assembly::Scaffold->get_all_seq_ids [dbolser, fangly]
* [2599] - support for DBLINK annotation in GenBank files [cjfields]
* [2594] - Bio::Species memory leak [cjfields]
* [2515] - GenBank XML parser [jhannah]
* [2499] - Method "pi" in package Bio::PopGen::Statistics [hyphaltip]
* [2483] - Bio::Assembly::IO::ace write_assembly implemented [fangly]
* [2350] - ID consistency btwn Bio::SeqI, Bio::Align::AlignI [fangly,
* [1572] - no docs Bio::Location::Simple/Atomic::trunc [hyphaltip]
* Bio::Expression modules - these were originally designed to go with the
bioperl-microarray suite of tools, however they have never been completed
and so have been removed from the distribution. The original code has
been moved into the inactive bioperl-microarray suite. [cjfields]
* Repository moved from Subversion (SVN) to [cjfields]
* Bug database has moved to Redmine (
* Bio::Micrarray - the tools developed for ReSeq chip analysis by Marian
Thieme have been moved to their own distribution (Bio-Microarray).
1.6.1 Sept. 29, 2009 (point release)
* No change from last alpha except VERSION and doc updates [cjfields]
1.6.0_6 Sept. 27, 2009 (sixth 1.6.1 alpha)
* Fix for silent OBDA bug related to FASTA validation [cjfields]
1.6.0_5 Sept. 27, 2009 (fifth 1.6.1 alpha)
* Possible fix for RT 49950 (Strawberry Perl installation) [cjfields]
* [RT 50048] - removed redundant VERSION, which was borking CPANPLUS
* BioPerl.pod -> (Perl Best Practices) [cjfields]
1.6.0_4 Sept. 25, 2009 (fourth 1.6.1 alpha)
* WinXP test fixes [cjfields, maj]
* BioPerl.pod added for descriptive information, fixes CPAN indexing
* Minor doc fixes [cjfields]
1.6.0_3 Sept. 22, 2009 (third 1.6.1 alpha)
* Fix tests failing due to merging issues [cjfields]
* More documentation updates for POD parsing [cjfields]
1.6.0_2 Sept. 22, 2009 (second 1.6.1 alpha)
* Bio::Root::Build
- fix YAML meta data generation [cjfields]
1.6.0_1 Sept. 15, 2009 (first 1.6.1 alpha)
* Bio::Align::DNAStatistics
- fix divide by zero problem [jason]
* Bio::AlignIO::*
- bug 2813 - fix faulty logic to detect end-of-stream [cjfields]
* Bio::AlignIO::stockholm
- bug 2796 - fix faulty logic to detect end-of-stream [cjfields]
* Bio::Assembly::Tools::ContigSpectrum
- function to score contig spectrum [fangly]
* Bio::DB::EUtilities
- small updates [cjfields]
* Bio::DB::Fasta
- berkeleydb database now autoindexes wig files and locks correctly
* Bio::DB::HIV
- various small updates for stability; tracking changes to LANL
database interface [maj]
* Bio::DB::SeqFeature (lots of updates and changes)
- add Pg, SQLite, and faster BerkeleyDB implementations [lstein, scain]
- bug 2835 - patch [Dan Bolser]
- bug RT 44535 - patch FeatureFileLoader [Cathy Gresham]
* Bio::DB::SwissProt
- bug 2764 - idtracker() method [cjfields, courtesy Neil Saunders]
* Bio::Factory::FTLocationFactory
- mailing list bug fix [cjfields]
* Bio::LocatableSeq
- performance work on column_from_residue_number [hartzell]
* Bio::Matrix::IO::phylip
- bug 2800 - patch to fix phylip parsing [Wei Zou]
* Bio::Nexml
- Google Summer of Code project from Chase Miller - parsers for Nexml
file format [maj, chmille4]
* Bio::PopGen
- Make Individual, Population, Marker objects AnnotatableI [maj]
- simplify LD code [jason]
* Bio::RangeI
- deal with empty intersection [jason]
* Bio::Restriction
- significant overhaul of Bio::Restriction system: complete support for
external and non-palindromic cutters. [maj]
* Bio::Root::Build
- CPANPLUS support, no automatic installation [sendu]
* Bio::Root::IO
- allow IO::String (regression fix) [cjfields]
- catch unintentional undef values [cjfields]
- throw if non-fh is passed to -fh [maj]
* Bio::Root::Root/RootI
- small debugging and core fixes [cjfields]
* Bio::Root::Test
- bug RT 48813 - fix for Strawberry Perl bug [kmx]
* Bio::Root::Utilities
- bug 2737 - better warnings [cjfields]
* Bio::Search
- tiling completely refactored, HOWTO added [maj]
NOTE : Bio::Search::Hit::* classes do not use this code directly; we
will deprecate usage of the older tiling code in the next BioPerl
- small fixes [cjfields]
* Bio::SearchIO
- Infernal 1.0 output now parsed [cjfields]
- new parser for gmap -f9 output [hartzell]
- bug 2852 - fix infinite loop in some output [cjfields]
- blastxml output now passes all TODO tests [cjfields]
- bug 2346, 2850 - psl and exonerate parsing fixes [rbuels, jhannah, bvecchi, YAPC hackathon]
- RT 44782 - GbrowseGFF writer now catches evalues [Allen Day]
- bug 2575 - add two columns of additional output to HSPTableWriter [cjfields]
* Bio::Seq::LargePrimarySeq
- delete tempdirs [cjfields]
- bug fixes [rbuels, jhannah, bvecchi, YAPC hackathon]
* Bio::Seq::Quality
- extract regions based on quality threshold value [Dan Bolser, heikki]
- bug 2847 - resolve threshold issue (rbuels, jhannah, bvecchi)
* Bio::SeqFeature::Lite
- various Bio::DB::SeqFeature-related fixes [lstein]
* Bio::SeqFeature::Tools::TypeMapper
- additional terms for GenBank to SO map [scain]
* Bio::SeqIO::chadoxml
- bug 2785 - patch to get this working for bp_seqconvert [cjfields]
* Bio::SeqIO::embl
- support for CDS records [dave_messina, Sylvia]
* Bio::SeqIO::fastq
- complete refactoring to handle all FASTQ variants, perform validation,
write output. API now conforms with other Bio* parsers and the rest of
Bio::SeqIO (e.g. write_seq() creates fastq output, not fasta output).
* Bio::SeqIO::genbank
- bug 2784 - fix DBSOURCE issue [Phillip Garland]
- bug RT 44536 - support for UniProt/UniProtKB tests [cjfields]
* Bio::SeqIO::largefasta
- parser returns a Bio::Seq::LargePrimarySeq [jhannah]
* Bio::SeqIO::raw
- add option for 'single' and 'multiple'
* Bio::SeqIO::scf
- bug 2881 - fix scf round-tripping [Adam Sj¿gren]
* Bio::SeqUtils
- bug 2766, 2810 - copy over tags from features, doc fixes [David
* Bio::SimpleAlign
- bug 2793 - patch for add_seq index issue [jhannah, maj]
- bug 2801 - throw if args are required [cjfields]
- bug 2805 - uniq_seq returns SimpleAlign and hash ref of sequence types
[Tristan Lefebure, maj]
- bug fixes from YAPC hackathon [rbuels, jhannah, bvecchi]
- fix POD and add get_SeqFeatures filter [maj]
* Bio::Tools::dpAlign
- add support for LocatableSeq [ymc]
- to be moved to a separate distribution [cjfields, rbuels]
* Bio::Tools::EUtilities
- fix for two bugs from mail list [Adam Whitney, cjfields]
- add generic ItemContainerI interface for containing same methods
* Bio::Tools::HMM
- fix up code, add more warnings [cjfields]
- to be moved to a separate distribution [cjfields, rbuels]
* Bio::Tools::Primer3
- bug 2862 - fenceposting issue fixed [maj]
* Bio::Tools::Run::RemoteBlast
- tests for remote RPS-BLAST [mcook]
* Bio::Tools::SeqPattern
- bug 2844 - backtranslate method [rbuels, jhannah, bvecchi]
* Bio::Tools::tRNAscanSE
- use 'gene' and 'exon' for proper SO, ensure ID is unique [jason]
* Bio::Tree::*
- bug 2456 - fix reroot_tree(), added create_node_on_branch() [maj]
* Bio::Tree::Statistics
- several methods for calculating Fitch-based score, internal trait
values, statratio(), sum of leaf distances [heikki]
* Bio::Tree::Tree
- bug 2869 - add docs indicating edge case where nodes can be
prematurely garbage-collected [cjfields]
- add as_text() function to create Tree as a string in specified format
* Bio::Tree::TreeFunctionsI
- bug 2877 - fix bug where bootstrap assigned to the wrong node [Tristan
Lefebure, maj]
* Bio::TreeIO::newick
- fix small semicolon issue [cjfields]
* scripts
- update to bp_seqfeature_load for SQLite [lstein]
- - commmand-line interface to Bio::DB::HIV [maj]
- fastam9_to_table - fix for MPI output [jason]
- gccalc - total stats [jason]
* General Stuff
- POD cleanup re: FEEDBACK section [maj, cjfields]
- cleanup or fix dead links [cjfields]
- Use of no_* methods (indicating 'number of something') is deprecated
in favor of num_* [cjfields]
- lots of new tests for the above bugs and refactors [everyone!]
- new template for Komodo text editor [cjfields]
1.6.0 Winter 2009
* Feature/Annotation rollback
- Problematic changes introduced prior to the 1.5 release have been
rolled back. These changes led to subtle bugs involving operator
overloading and interface methods.
- Behavior is very similar to that for BioPerl 1.4, with tag values
being stored generically as simple scalars. Results in a modest
* Bio::Graphics
- Split into a separate distribution on CPAN, primarily so development
isn't reliant on a complete BioPerl release.
- Bio::Graphics::Pictogram has been renamed to Bio::Draw::Pictogram but
is only available via Subversion (via bioperl-live main trunk)
* Bio::Root::Test
- Common test bed for all BioPerl modules
* Bio::Root::Build
- Common Module::Build-based subclass for all BioPerl modules
* Bio::DB::EUtilities
- Complete refactoring to split up parsing (Bio::Tools::EUtilities),
parameter handling (Bio::Tools::EUtilities::EUtilParameters),
and user agent request posting and retrieval
* Test implementation and reorganization
- Tests have been reorganized into groups based on classes or use
- Automated test coverage is now online:
- After this release, untested modules will be moved into a
separate developer distribution until tests can be derived.
Also, new modules to be added are expected to have a test suite
and adequate test coverage.
1.5.2 Developer release
Full details of changes since 1.5.1 are available online at:
The following represents a brief overview of the most important changes.
o Bio::Map
- Overhaul. Brand new system fully allows markers to have multiple
positions on multiple maps, and to have relative positions. Should be
backward compatible.
o Bio::Taxonomy
- This module and all the modules in the Taxonomy directory now
deprecated in favour of Bio::Taxon and Bio::Tree::Tree
o Bio::DB::Taxonomy
* get_Taxonomy_Node() eventually to be deprecated, renamed get_taxon().
* New methods ancestor(), each_Descendent() and _handle_internal_id().
* Allows for different database modules to create Bio::Taxon objects
with the same internal id when the same taxon is requested from each.
* get_Children_Taxids() is deprecated, superceded by each_Descendent().
* No longer includes the fake root node 'root'; there are multiple roots
now (10239, 12884, 12908, 29384 and 131567). Consistent with
* get_node() has new option -full
* Caches data retrieved from website
o Bio::Species
- Now a Bio::Taxon. Carries out the species name -> specific name munging
that Bio::DB::Taxonomy modules and SeqIO modules used to do, for
backward compatability in species() method.
o Bio::Search and Bio::SearchIO
- Overhaul. The existing system has been sped up via some minor changes
(mostly gain-of-function to the API). Bio::PullParserI is introduced
as a potential eventual replacment for the existing system, though as
yet only a Hmmpfam parser exists written using it.
1.5.1 Developer release
o Major problem with how Annotations were written out with
Bio::Seq is fixed by reverting to old behavior for
Bio::Annotation objects.
o Bio::SeqIO
* bug #1871; REFLOOP' parsing loop, I changed the pattern to
expect at l east 9 spaces at the beginning of a line to
indicate line wrapping.
* Treat multi-line SOURCE sections correctly, this defect broke
both common_name() and classification()
* parse swissprot fields in genpept file
* parse WGS genbank records
* Changed regexp for ID line. The capturing parentheses are
the same, the difference is an optional repeated-not-semi-
colon expression following the captured \S+. This means the
regexp works when the division looks like /PRO;/ or when the
division looks like /ANG ;/ - the latter is from EMBL
* fix ID line parsing: the molecule string can have spaces in
it. Like: "genomic DNA"
- bugs #1727, #1734
* Added parser for entrezgene ASN1 (text format) files.
Uses Bio::ASN1::EntrezGene as a low level parser (get it from CPAN)
o Bio::AlignIO
- coordinate problem fixed
o Bio::Taxonomy and Bio::DB::Taxonomy
- Parse NCBI XML now so that nearly all the taxonomy up-and-down
can be done via Web without downloading all the sequence.
o Bio::Tools::Run::RemoteBlast supports more options and complies
to changes to the NCBI interface. It is reccomended that you
retrieve the data in XML instead of plain-text BLAST report to
insure proper parsing and retrieval of all information as NCBI
fully expects to change things in the future.
o Bio::Tree and Bio::TreeIO
- Fixes so that re-rooting a tree works properly
- Writing out nhx format from a newick/nexus file will properly output
bootstrap information. The use must move the internal node labels over
to bootstraps.
for my $node ( grep { ! $_->is_Leaf } $tree->get_nodes ) {
- Nexus parsing is much more flexible now, does not care about
- Cladogram drawing module in Bio::Tree::Draw
- Node height and depth now properly calculated
- fix tree pruning algorithm so that node with 1 child gets merged
o Graphics tweaks. Glyph::xyplot improved. Many other small-medium sized
bugs and improvements were added, see Gbrowse mailing list for most of
o Bio::DB::GFF partially supports GFF3. See information about
gff3_munge flag in scripts/Bio-DB-GFF/
o Better location parsing in Bio::Factory::FTLocationFactory -
this is part of the engine for parsing EMBL/GenBank feature table
locations. Nested join/order-by/complement are allowed now
o Bio::PrimarySeqI->translate now takes named parameters
o Bio::Tools::Phylo::PAML - parsing RST (ancestral sequence
reconstruction) is now supported. Parsing different models and
branch specific parametes are now supported.
o Bio::Factory::FTLocationFactory - parse hierarchical locations
(joins of joins)
o Bio::Matrix::DistanceMatrix returns arrayrefs instead of arrays
for getter/setter functions
o Bio::SearchIO
- blast bug #1739; match scientific notation in score
and possible e+ values
- reads more WU-BLAST parameters and parameters, match
a full database pathname,
- Handle NCBI WEB and newer BLAST formats specifically
(Query|Sbjct:) match in alignment blocks can now be (Query|Sbjct).
- psl off-by-one error fixed
- exonerate parsing much improved, CIGAR and VULGAR can be parsed
and HSPs can be constructed from them.
- HSPs query/hit now have a seqdesc field filled out (this was
always available via $hit->description and
- can parse -A0 hmmpfam files
- Writer::GbrowseGFF more customizeable.
o Bio::Tools::Hmmpfam
make e-value default score displayed in gff, rather than raw score
allow parse of multiple records
1.5 Developer release
o Bio::Align::DNAStatistics and Bio::Align::ProteinStatistics
provide Jukes-Cantor and Kimura pairwise distance methods,
o Bio::AlignIO support for "po" format of POA, and "maf";
Bio::AlignIO::largemultifasta is a new alternative to
Bio::AlignIO::fasta for temporary file-based manipulation of
particularly large multiple sequence alignments.
o Bio::Assembly::Singlet allows orphan, unassembled sequences to
be treated similarly as an assembled contig.
o Bio::CodonUsage provides new rare_codon() and probable_codons()
methods for identifying particular codons that encode a given
amino acid.
o Bio::Coordinate::Utils provides new from_align() method to build
a Bio::Coordinate pair directly from a
Bio::Align::AlignI-conforming object.
o Bio::DB::Biblio::eutils is a class for querying NCBI's Eutils.
Send a Pubmed, Pubmed Central, Entrez, or other query to NCBI's
web service using standard Pubmed query syntax, and retrieve
results as XML.
o Bio::DB::GFF has various sundry bug fixes.
o Bio::FeatureIO is a new SeqIO-style subsystem for
writing/reading genomic features to/from files. I/O classes
exist for BED, GTF (aka GFF v2.5), and GFF v3. Bio::FeatureIO
classes only read/write Bio::SeqFeature::Annotated objects.
Notably, the GFF v3 class requires features to be typed into the
Sequence Ontology.
o Bio::Graph namespace contains new modules for manipulation and
analysis of protein interaction graphs.
o Bio::Graphics has many bug fixes and shiny new glyphs.
o Bio::Index::Hmmer and Bio::Index::Qual provide multiple-file
indexing for HMMER reports and FASTA qual files, respectively.
o Bio::Map::Clone, Bio::Map::Contig, and Bio::Map::FPCMarker are
new objects that can be placed within a Bio::Map::MapI-compliant
genetic/physical map; Bio::Map::Physical provides a new physical
map type; Bio::MapIO::fpc provides finger-printed clone mapping
o Bio::Matrix::PSM provide new support for postion-specific
(scoring) matrices (e.g. profiles, or "possums").
o Bio::Ontology::Ontology and Bio::Ontology::Term objects can now
be instantiated without explicitly using Bio::OntologyIO. This
is possible through changes to Bio::Ontology::OntologyStore to
download ontology files from the web as necessary. Locations of
ontology files are hard-coded into
o Bio::PopGen includes many new methods and data types for
population genetics analyses.
o New constructor to Bio::Range, unions(). Given a list of
ranges, returns another list of "flattened" ranges --
overlapping ranges are merged into a single range with the
mininum and maximum coordinates of the entire overlapping group.
o Bio::Root::IO now supports -url, in addition to -file and -fh.
The new -url argument allows one to specify the network address
of a file for input. -url currently only works for GET
requests, and thus is read-only.
o Bio::SearchIO::hmmer now returns individual Hit objects for each
domain alignment (thus containing only one HSP); previously
separate alignments would be merged into one hit if the domain
involved in the alignments was the same, but this only worked
when the repeated domain occured without interruption by any
other domain, leading to a confusing mixture of Hit and HSP
o Bio::Search::Result::ResultI-compliant report objects now
implement the "get_statistics" method to access
Bio::Search::StatisticsI objects that encapsulate any
statistical parameters associated with the search (e.g. Karlin's
lambda for BLAST/FASTA).
o Bio::Seq::LargeLocatableSeq combines the functionality already
found in Bio::Seq::LargeSeq and Bio::LocatableSeq.
o Bio::SeqFeature::Annotated is a replacement for
Bio::SeqFeature::Generic. It breaks compliance with the
Bio::SeqFeatureI interface because the author was sick of
dealing with untyped annotation tags. All
Bio::SeqFeature::Annotated annotations are Bio::AnnotationI
compliant, and accessible through Bio::Annotation::Collection.
o Bio::SeqFeature::Primer implements a Tm() method for primer
melting point predictions.
o Bio::SeqIO now supports AGAVE, BSML (via SAX), CHAOS-XML,
InterProScan-XML, TIGR-XML, and NCBI TinySeq formats.
o Bio::Taxonomy::Node now implements the methods necessary for
Bio::Species interoperability.
o Bio::Tools::CodonTable has new reverse_translate_all() and
make_iupac_string() methods.
o Bio::Tools::dpAlign now provides sequence profile alignments.
o Bio::Tools::GFF now parses GFF version 2.5 (a.k.a. GTF).
o Bio::Tools::Fgenesh, Bio::Tools::tRNAscanSE are new report
o Bio::Tools::SiRNA includes two new rulesets (Saigo and Tuschl)
for designing small inhibitory RNA.
o Bio::Tree::DistanceFactory provides NJ and UPGMA tree-building
methods based on a distance matrix.
o Bio::Tree::Statistics provides an assess_bootstrap() method to
calculate bootstrap support values on a guide tree topology,
based on provided bootstrap tree topologies.
o Bio::TreeIO now supports the Pagel (PAG) tree format.
1.4 branch
o Improvements to Bio::AlignIO::nexus for parsing TreeBase nexus files
o Bio::Graphics will work with gd1 or gd2
o Bio::SearchIO
- Better hmmpfam parsing, fix bug for small number of alignment outputs
(RF lines alone)
- Parse multi-line query fields properly
- small speed improvements to and others
o Bio::DB::Taxonomy has better support for hierarchy traversal so that
Bio::Taxonomy::Node can be as simple as Bio::Species object while still
supporting more complex queries
1.4. Stable major release
Since initial 1.2.0, 3000 separate changes have been made to make this release.
o installable scripts
o global module version from Bio::Root:Version
o Bio::Graphics
- major improvements; SVG support
o Bio::Popgen
- population genetics
- support several population genetics types of questions.
- Tests for statistical neutrality of mutations
(Fu and Li's D/F, Tajima's D) are in Bio::PopGen::Statistics.
Tests of population structure (Wright's F-statistic: Fst) is in
Bio::PopGen::PopStats. Calculating composite linkage
disequilibrium (LD) is available in Bio::PopGen::Statistics as
- Bio::PopGen::IO for reading in prettybase (SeattleSNPs)
and csv (comma delimited formatted) data.
- a directory for implementing population simulations has
been added Bio::PopGen::Simulation and 2 simulations - a
Coalescent and a simple single-locus multi-allele genetic drift
simulation have been provided. This replaces the code in
Bio::Tree::RandomTree which has been deprecated until proper
methods for generating random phylogenetic trees are
o Bio::Restriction
- new restrion analysis modules
o Bio::Tools::Analysis
- web based DNA and Protein analysis framework and several
o Bio::Seq::Meta
- per residue annotable sequences
o Bio::Matrix
- Bio::Matrix::PSM - Position Scoring Matrix
- Bio::Matrix::IO has been added for generalized parsing of
matrix data. Matrix::IO::scoring and Matrix::IO::phylip are
initial implementations for parsing BLOSUM/PAM and Phylip
Distance matricies respectively. A generic matrix
implementation for general use was added in
o Bio::Ontology
- major changes
o Bio:Tree
o Bio::Tools::SiRNA, Bio::SeqFeature::SiRNA
- small inhibitory RNA
o Bio::SeqFeature::Tools
- seqFeature mapping tools
-- deal with mapping GenBank feature collections into
Chado/GFF3 processable feature sets (with SO term mappings)
o Bio::Tools::dpAlign
- pure perl dynamic programming sequence alignment
- needs Bioperl-ext
o new Bio::SearchIO formats
- axt and psl: UCSC formats.
- blasttable: NCBI -m 8 or -m 9 format from blastall
o new Bio::SeqIO formats
- chado, tab, kegg, tigr, game
- important fixes for old modules
o Bio::AlignIO: maf
o improved Bio::Tools::Genewise
o Bio::SeqIO now can recongnize sequence formats automatically from
o new parsers in Bio::Tools:
Blat, Geneid, Lagan, Mdust, Promoterwise, PrositeScan,
o Bio::DB::Registry bugs fixed
- BerkeleyDB-indexed flat files can be used by the OBDA system
- Multiple seqdatabase.ini locations in OBDA_SEARCH_PATH are all
used by the OBDA system
o several new HOWTOs
- SimpleWebAnalysis, Trees, Feature Annotation, OBDA Access, Flat
o hundreds of new and improved files
o Bio::Tree::AlleleNode has been updated to be a container of
an Bio::PopGen::Individual object for use in the Coalescent simulations.
1.2 Branch
1.2.3 Stable release update
o Bug #1475 - Fix and add speedup to spliced_seq for remote location
o Bug #1477 - Sel --> Sec abbreviation fixed
o Fix bug #1487 where paring in-between locations when
end < start caused the FTLocationFactory logic to fail.
o Fix bug #1489 which was not dealing with keywords as an
arrayref properly (this is fixed on the main trunk because
keywords returns a string and the array is accessible via
o Bio::Tree::Tree memory leak (bug #1480) fixed
Added a new initialization option -nodelete which
won't try and cleanup the containing nodes if this
is true.
o Bug with parsing labeled nodes with Bio::TreeIO::newick fixed
this was only present on the branch for the 1.2.1 and 1.2.2 series
- Also merged main trunk changes to the branch which make
newick -> nhx round tripping more effective (storing branch length
and bootstrap values in same locate for NodeNHX and Node
implementations.) Fixes to TreeIO parsing for labeled internal
also required small changes to TreeIO::nhx. Improved
tests for this module as well.
o Bio::SearchIO
- Fixed bugs in BLAST parsing which couldn't parse NCBI
gapped blast properly (was losing hit significance values due to
the extra unexpeted column).
- Parsing of blastcl3 (netblast from NCBI) now can handle case of
integer overflow (# of letters in nt seq dbs is > MAX_INT)
although doesn't try to correct it - will get the negative
number for you. Added a test for this as well.
- Fixed HMMER parsing bug which prevented parsing when a hmmpfam report
has no top-level family classification scores but does have scores and
alignments for individual domains.
- Parsing FASTA reports where ungapped percent ID is < 10 and the
regular expression to match the line was missing the possibility of
an extra space. This is rare, which is why we probably did not
catch it before.
- BLAST parsing picks up more of the statistics/parameter fields
at the bottom of reports. Still not fully complete.
- SearchIO::Writer::HTMLResultWriter and TextResultWriter
were fixed to include many improvements and added flexiblity
in outputting the files. Bug #1495 was also fixed in the process.
o Bio::DB::GFF
- Update for GFF3 compatibility.
- Added scripts for importing from UCSC and GenBank.
- Added a 1.2003 version number.
o Bio::Graphics
- Updated tutorial.
- Added a 1.2003 version number.
o SeqIO::swiss Bug #1504 fixed with swiss writing which was not
properly writing keywords out.
o Bio::SeqIO::genbank
- Fixed bug/enhancement #1513 where dates of
the form D-MMM-YYYY were not parsed. Even though this is
invalid format we can handle it - and also cleanup the date
string so it is properly formatted.
- Bug/enhancement #1517 fixed so that SEGMENT line can be parsed
and written with Genbank format. Similarly bug #1515 is fixed to
parse in the ORIGIN text.
o Bio::SeqIO::fasta, a new method called preferred_id_type allows you
to specify the ID type, one of (accession accession.version
display primary). See Bio::SeqIO::preferred_id_type method
documentation for more information.
o Unigene parsing updated to handle file format changes by NCBI
1.2.2 Stable release update
o A series of bug fixes of the Bio::OntologyIO dagflat-related parsers:
- auto-discover ontology name
- bug in parsing relationships when certain characters are in the term
- fixed hard-coded prefix for term identifiers
- various smaller issues
o Fixed bug in Bio::Annotation::OntologyTerm of not implementing all
of Bio::Ontology::TermI
o brought the OBDA Registry code up to latest specs
o Bio::DB::GenBank
- eutils URL change
- accession number retrieval fixed
o Bio::SearchIO::blast - fix bug #1443 (missing last hits), parse megablast
o Bio::SearchIO::Writer::(HTML|Text)ResultWriter fix bugs #1458,
#1459 which now properly report alignment start/end info
for translated BLAST/FASTA searches.
o Bio::TreeIO::newick can parse labeled internal nodes
o Bio::Tools::BPbl2seq can properly report strand info for HSPs
for BLASTX if if you provide -report_type => 'BLASTX' when
initializing a BPbl2seq object. Bioperl 1.3 will have better
support for bl2seq in the SearchIO system.
o Bio::Root::IO support a -noclose boolean flag which will not
close a filehandle upon object cleanup - useful when sharing
a filehandle among objects. Additionally code added s.t.
STDOUT/STDIN/STDERR will never be closed by Root::IO cleanup.
o Bio::Tools::Genemark bug #1435 fixed which was missing last prediction
o Bio::SeqIO::genbank
- bug #1456 fixed which generated extra sequence lines
- write moltype correctly for genpept
1.2.1 Stable release update
o Inclusion of WrapperBase, a needed component for StandAloneBlast
o Addition from main trunk of Ontology objects, principly to allow
BioSQL releases against 1.2.1
o Fixes and cleanup of Bio::Coordinate modules
o A fix to Bio::Index::EMBL allowing retrieval of entries using
the primary accession number
o Other bug fixes, including bpindex GenBank fix
o Bio::SeqIO::genbank bug #1389 fixed
1.2 Stable major release
o More functionality added to Bio::Perl, the newbie module
o Bug fixes in Bio::TreeIO::newick fixes bug introduced in 1.0.2
Support for New Hampshire Extended (NHX) format parsing.
o Bio::Tools added support for parsing Genomewise, Pseudowise, Est2Genome,
Tmhmm, SignalP, Seg, RepeatMasker, FootPrinter, and a lightweight
Hmmpfam parser.
o New ontology parsing Bio::Ontology
o Bug fixes in Bio::SearchIO for HMMer parsing, support for
multi-report (mlib) fasta reports, support for waba and exonerate.
o Bio::ClusterIO for parsing Unigene clusters
o Bio::Assembly added for representing phrap and ace assembly clusters.
o Rudimentary support for writing Chado XML (see
GMOD project: for more information)
o Bio::Coordinate for mapping between different coordinate systems such
as protein -> cDNA -> Exon -> DNA and back. Useful for mapping
features into different coordinate systems.
o Bio::DB::GenBank/Bio::DB::GenPept now support Entrez queries
with the get_Stream_by_query method and supports the latest
NCBI eutils interface.
o Bugs fixed in Bio::SeqFeature::Collection an in-memory fast
object for extracting subsets of features : currently only
supports extraction by location.
1.1.1 Developer release
o Deprecated modules are now listed in the DEPRECATED file
o New HowTo documents located in doc/howto describing
a domain of Bioperl.
o Note that bugs are now stored at
and all old bugs are searchable through the bugzilla interface.
o Several reported bugs in Bio::Tools::Sigcleave and Bio::SimpleAlign
have been addressed.
o Support for Genewise parsing in Bio::Tools::Genewise
o Start of Ontology framework with Bio::Ontology
o Speedup to the Bio::Root::Root object method _rearrange.
A global _load_module method was implemented to simplify the
dynamic loading of modules ala Bio::SeqIO::genbank. This
method is now used by all the XXIO (AlignIO,TreeIO,SearchIO,SeqIO,
o Several performance improvements to sequence parsing in Bio::SeqIO.
Attempt to speedup by reducing object creation overhead.
o Bio::DB::GenBank and Bio::DB::GenPept use the NCBI's approved
method for sequence retrieval with their E-utils CGI scripts.
More work to support Entrez queries to their fullest is planned
before 1.2 release.
o Numerous fixes to Bio::SearchIO and sequence parsing (swissprot)
1.1 Developer release
o Bio::Tools::Run has been broken off into a new pkg bioperl-run,
this separation removes some of the complexity in our test suite
and separates the core modules in bioperl from those that need
external programs to run.
o With latest ExtUtils::MakeMaker module installed SGI/IRIX should
not run into trouble running the makefile
o Bio::Location and Bio::SeqIO::FTHelper are fixed to properly
read,create,and write locations for grouped/split locations
(like mRNA features on genomic sequence).
o Bio::Tools::Phlyo added for wrappers for parsing Molphy (protml)
and PAML (codeml,aaml, etc) parsing.
o Bio::Tree:: objects expanded to handle testing monophyly,
paraphyly, least common ancestor, etc.
o Bio::Coordinate for mapping locations from different coordinate spaces
o Bio::SearchIO::waba added for parsing WABA, Bio::SearchIO::hmmer
added for parsing hmmpfam and hmmsearch output.
o Bio::SearchIO::Writer::TextResultWriter for outputting
a pseudo-blast textfile format
1.0.2 Bug fix release
o Note: The modules Bio::DB::GenBank and Bio::DB::GenPept provided
in this release will not work after December 2002 when NCBI
shuts off the old Entrez cgi scripts. We have already fixed
on our main development branch and the functionality will be
available in the next stable bioperl release (1.2) slated for
Fall 2002.
o Numerous parsing bugs in Bio::SearchIO::fasta found through
testset by Robin Emig. These were fixed as was the get_aln
method in Bio::Search::HSP::GenericHSP to handle the extra
context sequence that is provided with a FastA alignment.
o Migrating differences between Bio::Search::XX::BlastXX to
Bio::Search::XX::GenericXX objects. This included mechanism
to retrieve whole list of HSPs from Hits and whole list of Hits from
Results in addition to the current next_XX iterator methods that
are available. Added seq_inds() method to GenericHSP which identifies
indexes in the query or hit sequences where conserved,identical,gaps,
or mismatch residues are located (adapted from Steve Chervitz's
implementation in BlastHSP).
o Bio::DB::GFF bugs fixed and are necessary for latest GBrowse release.
Bio::DB::GFF::RelSegment is now Bio::SeqI compliant.
o Bio::Graphics glyph set improved and extended for GBrowse release
o Bio::Tree::Tree get_nodes implementation improvement thanks
to Howard Ross notice performance problem when writing out
unbalanced trees.
o Bio::Location::Fuzzy::new named parameter -loc_type became
-location_type, Bio::Location::Simple::new named parameter
-seqid becamse -seq_id.
o Fixed major Bio::AlignIO::emboss parsing bug on needle output,
was mis-detecting that gaps should be placed at the beginning of
the alignment when the best alignment starts internally in the
1.0.1 Bug fix release
o Minor bug fixes to Bio::DB:GFF. Glyph sets improved.
o Parser fixes in SearchIO blast, fasta for more complete WU BLAST
and mixed (3.3 - 3.4) versions of FASTA.
o Small API change to add methods for completeness across
implementations of Bio::Search objects. These new methods
in the interface are implemented by the GenericXX object as well
as the BlastXX objects.
* Bio::Search::Result::ResultI
- hits() method returns list of all Hits (next_hit is an
iterator method)
* Bio::Search::Hit::HitI
- hsps() method returns list of all HSPs (next_hsp is an
iterator method)
o The Bio::SearchIO::Writer classes have been fixed to handle results
created from either psiblast (Search::BlastXX objects) or
blast|fasta|blastxml objects (Search::GenericXX objects). More work
has to be done here to make it work properly and will nee major
API changes.
o Bugs in Bio::Tools::HMMER fixed, including
* #1178 - Root::IO destructor wasn't being called
* #1034 - filter_on_cutoff now behaves properly
o Bio::SeqFeature::Computation initialization args fixed and
tests added.
o Tests are somewhat cleaner, flat.t now properly cleans up after itsself,
o Updated FAQ with more example based answers to typical questions
o Bug #1202 was fixed which would improperly join together qual values
parsed by Bio::SeqIO::qual when a trailing space was not present before
the newline.
1.0.0 Major Stable Release
This represents a major release of bioperl with significant
improvements over the 0.7.x series of releases.
o Bio::Tools::Blast is officially deprecated. Please see
Bio::SearchIO for BLAST and FastA parsing.
o The methods trunc() and subseq() in Bio::PrimarySeqI now accepts
Bio::LocationI objects as well as start/end.
o Bio::Biblio contains modules for Bibliographic data.
Bio::DB::Biblio contains the query modules. Additionally one can
parse medlinexml from the ebi bibliographic query service (BQS)
system and Pubmed xml from NCBI. See Martin Senger's
documentation in Bio::Biblio for more information.
o Bio::DB::Registry is a sequence database registry part of
Open Bioinformatics Database Access. See for more information.
o File-based and In-Memory Sequence caching is provided by
Bio::DB::InMemoryCache and Bio::DB::FileCache which acts like a
local database.
o Bio::Graphics for rendering sequences as PNG,JPG, or GIFs has
been added by Lincoln Stein.
o XEMBL SOAP service access in provided in Bio::DB::XEMBL.
o A FAQ has been started and is included in the release to provide
a starting point for frequent questions and issues.
0.9.3 Developer's release
o Event based parsing system improved (SearchIO). With parsers for
XML Blast (blastxml), Text Blast (blast), and FASTA results (fasta).
Additionally a lazy parsing system for text and html blast reports was
added and is called psiblast (name subject to change in future releases).
o Bio::Search objects improved and standardized with associated Interfaces
written. The concept of a search "Hit" was standardized to be called
"hit" consistently and the use of "subject" was deprecated in all active
o Bio::Structure added (since 0.9.1) for Protein structure objects
and PDB parser to retrieve and write these structures from data files.
o Several important Bio::DB::GFF bug fixes for handling features that
are mapped to multiple reference points. Updated mysql adaptor
so as to be able to store large (>100 megabase) chunks of DNA into
Bio::DB::GFF databases.
0.9.2 Developer's release
o Bio::Search and Bio::SearchIO system introduced for event based
parsing of Blast,Fasta reports Bio::SearchIO supports ncbi BLAST
in text and XML and FASTA reports in standard output format.
o Bio::Tree and Bio::TreeIO for phylogenetic trees. A Random tree
generator is included in Bio::TreeIO::RandomTrees and a
statistics module for evaluating.
o Bio::DB::GFF, Lincoln Stein's GFF database suitable as a DB
server for DAS servers.
o Bio::Tools::BPlite is provides more robust parsing of BLAST
files. The entire BPlite system migrated to using Bio::Root::IO
for the data stream.
o Bio::Tools::Alignment for Consed and sequence Trimming
o Bio::Structure for Protein structure information and parsing
o Bio::DB::GenBank/Bio::DB::GenPept updated to new NCBI Entrez
cgi-bin entry point which should be more reliable.
o Bio::Map and Bio::MapIO for biological map navigation and a
framework afor parsing them in. Only preliminary work here.
o Interface for executing EMBOSS programs locally in Bio::Factory::EMBOSS
Future work will integrate Pise and allow submission of analysis on
remote servers.
o Bio::AnnotationCollectionI and Bio::Annotation::Collection
introduced as new objects for handling Sequence Annotation
information (dblinks, references, etc) and is more robust that
previous system.
o Bio::Tools::FASTAParser introduced.
o Scripts from the bioperl script submission project and new
scripts from bioperl authors are included in "scripts" directory.
o Factory objects and interfaces are being introduced and are more
strictly enforced.
o Bio::Root::Root introduced as the base object while
Bio::Root::RootI is now simply an interface.
o Bio::DB::RefSeq provides database access to copy of the NCBI
RefSeq database using the EBI dbfetch script.
0.9.0 Developer's release
o perl version at least 5.005 is now required instead of perl 5.004
o Bio::Tools::Run::RemoteBlast is available for running remote
blast jobs at NCBI.
o Bio::Tools::BPbl2seq was fixed to handle multiple HSPs.
o Bio::SeqFeature::GeneStructure migrated to Bio::SeqFeature::Gene.
Also added are related modules UTR3, UTR5, Exon, Intron,
Promotor, PolyA and Transcript.
o Speedup of translate method in PrimarySeq
o Bio::SimpleAlign has new methods: location_from_column(), slice(),
select(), dot(), get_seq_by_pos(), column_from_residue_number()
o Various fixes to Variation toolkit
o Bio::DB::EMBL provides database access to EMBL sequence data.
Bio::DB::Universal provides a central way to point to indexes
and dbs in a single interface.
o Bio::DB::GFF - a database suitable for running DAS servers locally.
o Bio::Factory::EMBOSS is still in design phase as is
o Dia models for bioperl design are provided in the models/ directory
0.7.2 Bug fix release
o documentation fixes in many modules - SYNOPSIS code verified
to be runnable in many (but not all modules)
o corrected MANIFEST file from 0.7.1 release
o Bug fix in Bio::SeqIO::FTHelper to properly handle
split locations
o Bio::SeqIO::genbank
* Correct parsing and writing of genbank format with protein data
* moltype and molecule separation
o Bio::SeqIO::largefasta fix to avoid inifinite loops
o Bio::SimpleAlign fixed to correctly handle consensus
sequence calculation
o Bio::Tools::HMMER supports hmmer 2.2g
o Bio::Tools::BPlite to support report type specific parsing. Most
major changes are not on the 0.7 branch.
o Bio::Tools::Run::StandAloneBlast exists_blast() fixed and works
with File::Spec
o Bio::Variation::AAChange/RNAChange corrected labels and mutated alleles
in several types of mutations:
1.) AA level: deletion, complex
2.) AA level: complex, inframe
3.) RNA level: silent
o BPbl2seq parsing of empty reports will not die, but will return
a valid, empty, Bio::SeqFeature::SimilarityFeature for
$report->query() and $report->subject() methods. So an easy
way to test if report was empty is to see if
$report->query->seqname is undefined.
0.7.1 Bug fix release
o Better parsing of genbank/EMBL files especially fixing bugs
related to Feature table parsing and locations on remote
sequences. Additionally, species name parsing was better.
o Bio::SeqIO::genbank can parse now NCBI produced genbank database
which include a number of header lines.
o More strict genbank and EMBL format writing (corrected number of
spaces where appropriate).
o Bio::Tools::BPlite can better parse BLASTX reports - see BUGS
for related BPlite BUGS that are unresolved in this release.
o Bio::DB::GenBank, Bio::DB::GenPept have less problems
downloading sequences from NCBI via HTTP. Bio::DB::SwissProt can
use expasy mirrors or EBI dbfetch cgi-script.
o A moderate number of documentation improvements were made as
well to provide a better code synopsis in each module.
0.7 Large number of changes, including refactoring of the
Object system, new parsers, new functionality and
all round better system. Highlights are:
o Refactored root of inheritance: moved to a lightweight Bio::Root::RootI;
Bio::Root::IO for I/O and file/handle capabilities.
o Imported BPlite modules from Ian Korf for BLAST
parsing. This is considered the supported BLAST parser; will eventually phase out due to lack of support.
o Improved Sequence Feature model. Added complete location
modelling (with fuzzy and compound locations). See
Bio::LocationI and the modules under Bio/Location. Added
support in Genbank/EMBL format parsing to completely parse
feature tables for complex locations.
o Moved special support for databanks etc to specialized modules under
Bio/Seq/. One of these supports very large sequences through
a temporary file as a backend.
o Explicit Gene, Transcript and Exon SeqFeature objects, supporting
CDS retrieval and exon shuffling.
o More parsers: Sim4, Genscan, MZEF, ESTScan, BPbl2seq, GFF
o Refactored Bio/DB/GenBank+GenPept. There is now also DB/SwissProt and
DB/GDB (the latter has platform-specific limitations).
o New analysis parser framework for HT sequence annotation (see
Bio::SeqAnalysisParserI and Bio::Factory::SeqAnalysisParserFactory)
o New Alignment IO framework
o New Index modules (Swissprot)
o New modules for running Blast within perl
(Bio::Tools::Run::StandAloneBlast). Added modules for running
Multiple Sequence Alignment tools ClustalW and TCoffee
o New Cookbook-style tutorial (see Improved
documentation across the package.
o Much improved cross platform support. Many known incompatibilities
have been fixed; however, NT and Mac do not work across the entire
setup (see PLATFORMS).
o Many bug fixes, code restructuring, etc. Overall stability and
maintainability benefit a lot.
o A total of 957 automatic tests
There are very few functionality changes but a large
number of software improvements/bug fixes across the package.
o The EMBL/GenBank parsing are improved.
o The Swissprot reading is improved. Swissprot writing
is disabled as it doesn't work at all. This needs to
wait for 0.7 release
o BLAST reports with no hits are correctly parsed.
o Several other bugs of the BLAST parser (regular expressions, ...)
o Old syntax calls have been replaced with more modern syntax
o Modules that did not work at all, in particular the Sim4
set have been removed
o Bio::SeqFeature::Generic and Bio::SeqFeature::FeaturePair
have improved compliance with interface specs and documentation
o Mailing list documentation updated throughout the distribution
o Most minor bug fixes have happened.
o The scripts in /examples now work and have the modern syntax
rather than the deprecated syntax
0.6.1 Sun April 2 2000
o Sequences can have Sequence Features attached to them
- The sequence features can be read from or written to
EMBL and GenBank style flat files
o Objects for Annotation, including References (but not
full medline abstracts), Database links and Comments are
o A Species object to represent nodes on a taxonomy tree
is provided
o The ability to parse HMMER and Sim4 output has been added
o The Blast parsing has been improved, with better PSI-BLAST
support and better overall behaviour.
o Flat file indexed databases provide both random access
and sequential access to their component sequences.
o A CodonTable object has been written with all known
CodonTables accessible.
o A number of new lightweight analysis tools have been
added, such as molecular weight determination.
The 0.6 release also has improved software engineering
o The sequence objects have been rewritten, providing more
maintainable and easier to implement objects. These
objects are backwardly compatible with the 0.05.1 objects
o Many objects are defined in terms of interfaces and then
a Perl implementation has been provided. The interfaces
are found in the 'I' files (module names ending in 'I').
This means that it is possible to wrap C/CORBA/SQL access
as true "bioperl" objects, compatible with the rest of
o The SeqIO system has been overhauled to provide better
processing and perl-like automatic interpretation of <>
over arguments.
o Many more tests have been added (a total of 172 automatic
tests are now run before release).
0.05.1 Tue Jun 29 05:30:44 1999
- Central distribution now requires Perl 5.004. This was
done to get around 5.003-based problems in Bio/Index/*
and SimpleAlign.
- Various bug fixes in the Bio::Tools::Blast modules
including better exception handling and PSI-Blast
support. See Bio/Tools/Blast/CHANGES for more.
- Fixed the Parse mechanism in to use readseq.
Follow the instructions in README for how to install
it (basically, you have to edit
- Improved documentation of, indicating where
objects are returned and where strings are returned.
- Fixed uninitialized warnings in
- Bug fixes for PR#s: 30,31,33-35,41,42,44,45,47-50,52.
0.05 Sun Apr 25 01:14:11 1999
- Bio::Tools::Blast modules have less memory problems
and faster parsing. Webblast uses LWP and supports
more functionality. See Bio/Tools/Blast/CHANGES for more.
- The Bio::SeqIO system has been started, moving the
sequence reformatting code out of the sequence object
- The Bio::Index:: system has been started, providing
generic index capabilities and specifically works for
Fasta formatted databases and EMBL .dat formatted
- The Bio::DB:: system started, providing access to
databases, both via flat file + index (see above) and
via http to NCBI
- The scripts/ directory, where industrial strength scripts
are put has been started.
- Many changes - a better distribution all round.
0.04.4 Wed Feb 17 02:20:13 1999
- Bug fixes in the Bio::Tools::Blast modules and
(see Bio::Tools::Blast::CHANGES).
- Fixed a bug in Bio::Tools::Fasta::num_seqs().
- Beefed up the t/Fasta.t test script.
- Small fix in Bio::Seq::type() (now always returns a string).
- Changed Bio::Root::Utilities::get_newline_char() to
get_newline() since it could return more than one char.
- Added $NEWLINE and $TIMEOUT_SECS to Bio::Root::Global.
- Changed default timeout to 20 seconds (was 3).
- Moved lengthy modification notes to the bottom of some files.
- Fixed SimpleAlign write_fasta bug.
- Beefed up SimpleAlign.t test
0.04.3 Thu Feb 4 07:48:53 1999
- and now detect when
script is run as a CGI and suppress output that is only
appropriate when running interactively.
- Bio::Root::Err::_set_context() adds name of script ($0).
- Added comments in and
regarding the use of the static objects via the qw(:obj) tag.
- Fixed the ambiguous reverse calls in and to
CORE::reverse, avoiding Perl warnings.
- Bug fixes in Bio::Tools::Blast modules (version 0.074) and
example scripts (see Bio::Tools::Blast::CHANGES).
- examples/seq/ no longer always warns about using
-prot or -nucl command-line arguments; only when using the
-debug argument.
- Methods added to Bio::Root::Utilities: create_filehandle(),
get_newline_char(), and taste_file() to generalize filehandle
creation and autodetect newline characters in files/streams
(see bug report #19).
- Bio::Root::IOManager::read() now handles timeouts and uses
- uses Utilities::get_newline_char() instead
of hardwiring in "\n".
- Bug fixes in the Bio::SimpleAlign and Bio::Tools::pSW
0.04.2 Wed Dec 30 02:27:36 1998
- Bug fixes in Bio::Tools::Blast modules, version 0.073
(see Bio::Tools::Blast::CHANGES).
- Changed reverse calls in Bio/ and Bio/
to CORE::reverse (prevents ambiguous warnings with 5.005).
- Appending '.tmp.bioperl' to temporary files created by
Bio::Root::Utilities::compress() or uncompress() to
make it easy to identify & cleanup these files as needed.
- Developers: Created CVS branch release-0-04-bug from
release-0-04-1. Before making bug fixes to the 0.04.1 release,
be sure to cvs checkout this branch into a clean area.
0.04.1 Wed Dec 16 05:39:15 1998
- Bug fixes in Bio::Tools::Blast modules, version 0.072
(see Bio::Tools::Blast::CHANGES).
- Compile/SW/Makefile.PL now removes *.o and *.a files
with make clean.
0.04 Tue Dec 8 07:49:19 1998
- Lots of new modules added including:
* Ewan Birney's,,
and Bio/Compile directory containing XS-linked C code for
creating Smith-Waterman sequence alignments from within Perl.
* Steve Chervitz's Blast distribution has been incorporated.
* Georg Fuellen's for multiple alignment objects.
- Bio/examples directory for demo scripts for all included modules.
- Bio/t directory containing test suit for all included modules.
- For changes specific to the Blast-related modules prior to
incorporation in this central distribution, see the CHANGES
file in the Bio/Tools/Blast directory.
0.01 Tue Sep 8 14:23:22 1998
- original version from central CVS tree; created by h2xs 1.18
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