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# $Id$
# BioPerl module for Bio::Tools::Run::PiseApplication::restrict
#
# Cared for by Catherine Letondal <letondal@pasteur.fr>
#
# For copyright and disclaimer see below.
#
# POD documentation - main docs before the code
=head1 NAME
Bio::Tools::Run::PiseApplication::restrict
=head1 SYNOPSIS
#
=head1 DESCRIPTION
Bio::Tools::Run::PiseApplication::restrict
Bioperl class for:
RESTRICT Finds restriction enzyme cleavage sites (EMBOSS)
Parameters:
(see also:
http://bioweb.pasteur.fr/seqanal/interfaces/restrict.html
for available values):
restrict (String)
init (String)
sequence (Sequence)
sequence -- DNA [sequences] (-sequence)
pipe: seqsfile
sitelen (Integer)
Minimum recognition site length (-sitelen)
enzymes (String)
Comma separated enzyme list (-enzymes)
min (Integer)
Minimum cuts per RE (-min)
max (Integer)
Maximum cuts per RE (-max)
single (Switch)
Force single site only cuts (-single)
blunt (Switch)
Allow blunt end cutters (-blunt)
sticky (Switch)
Allow sticky end cutters (-sticky)
ambiguity (Switch)
Allow ambiguous matches (-ambiguity)
plasmid (Switch)
Allow circular DNA (-plasmid)
commercial (Switch)
Only enzymes with suppliers (-commercial)
datafile (String)
Alternative RE data file (-datafile)
limit (Switch)
Limits reports to one isoschizomer (-limit)
preferred (Switch)
Report preferred isoschizomers (-preferred)
alphabetic (Switch)
Sort output alphabetically (-alphabetic)
fragments (Switch)
Show fragment lengths (-fragments)
name (Switch)
Show sequence name (-name)
outfile (OutFile)
outfile (-outfile)
auto (String)
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to
the Bioperl mailing list. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via the
web:
http://bugzilla.open-bio.org/
=head1 AUTHOR
Catherine Letondal (letondal@pasteur.fr)
=head1 COPYRIGHT
Copyright (C) 2003 Institut Pasteur & Catherine Letondal.
All Rights Reserved.
This module is free software; you can redistribute it and/or modify
it under the same terms as Perl itself.
=head1 DISCLAIMER
This software is provided "as is" without warranty of any kind.
=head1 SEE ALSO
=over
=item *
http://bioweb.pasteur.fr/seqanal/interfaces/restrict.html
=item *
Bio::Tools::Run::PiseApplication
=item *
Bio::Tools::Run::AnalysisFactory::Pise
=item *
Bio::Tools::Run::PiseJob
=back
=cut
#'
package Bio::Tools::Run::PiseApplication::restrict;
use vars qw(@ISA);
use strict;
use Bio::Tools::Run::PiseApplication;
@ISA = qw(Bio::Tools::Run::PiseApplication);
=head2 new
Title : new()
Usage : my $restrict = Bio::Tools::Run::PiseApplication::restrict->new($location, $email, @params);
Function: Creates a Bio::Tools::Run::PiseApplication::restrict object.
This method should not be used directly, but rather by
a Bio::Tools::Run::AnalysisFactory::Pise instance.
my $factory = Bio::Tools::Run::AnalysisFactory::Pise->new();
my $restrict = $factory->program('restrict');
Example : -
Returns : An instance of Bio::Tools::Run::PiseApplication::restrict.
=cut
sub new {
my ($class, $location, $email, @params) = @_;
my $self = $class->SUPER::new($location, $email);
# -- begin of definitions extracted from /local/gensoft/lib/Pise/5.a/PerlDef/restrict.pm
$self->{COMMAND} = "restrict";
$self->{VERSION} = "5.a";
$self->{TITLE} = "RESTRICT";
$self->{DESCRIPTION} = "Finds restriction enzyme cleavage sites (EMBOSS)";
$self->{OPT_EMAIL} = 0;
$self->{CATEGORIES} = [
"nucleic:restriction",
];
$self->{DOCLINK} = "http://www.uk.embnet.org/Software/EMBOSS/Apps/restrict.html";
$self->{_INTERFACE_STANDOUT} = undef;
$self->{_STANDOUT_FILE} = undef;
$self->{TOP_PARAMETERS} = [
"restrict",
"init",
"input",
"required",
"advanced",
"output",
"auto",
];
$self->{PARAMETERS_ORDER} = [
"restrict",
"init",
"input", # input Section
"sequence", # sequence -- DNA [sequences] (-sequence)
"required", # required Section
"sitelen", # Minimum recognition site length (-sitelen)
"enzymes", # Comma separated enzyme list (-enzymes)
"advanced", # advanced Section
"min", # Minimum cuts per RE (-min)
"max", # Maximum cuts per RE (-max)
"single", # Force single site only cuts (-single)
"blunt", # Allow blunt end cutters (-blunt)
"sticky", # Allow sticky end cutters (-sticky)
"ambiguity", # Allow ambiguous matches (-ambiguity)
"plasmid", # Allow circular DNA (-plasmid)
"commercial", # Only enzymes with suppliers (-commercial)
"datafile", # Alternative RE data file (-datafile)
"output", # output Section
"limit", # Limits reports to one isoschizomer (-limit)
"preferred", # Report preferred isoschizomers (-preferred)
"alphabetic", # Sort output alphabetically (-alphabetic)
"fragments", # Show fragment lengths (-fragments)
"name", # Show sequence name (-name)
"outfile", # outfile (-outfile)
"auto",
];
$self->{TYPE} = {
"restrict" => 'String',
"init" => 'String',
"input" => 'Paragraph',
"sequence" => 'Sequence',
"required" => 'Paragraph',
"sitelen" => 'Integer',
"enzymes" => 'String',
"advanced" => 'Paragraph',
"min" => 'Integer',
"max" => 'Integer',
"single" => 'Switch',
"blunt" => 'Switch',
"sticky" => 'Switch',
"ambiguity" => 'Switch',
"plasmid" => 'Switch',
"commercial" => 'Switch',
"datafile" => 'String',
"output" => 'Paragraph',
"limit" => 'Switch',
"preferred" => 'Switch',
"alphabetic" => 'Switch',
"fragments" => 'Switch',
"name" => 'Switch',
"outfile" => 'OutFile',
"auto" => 'String',
};
$self->{FORMAT} = {
"init" => {
"perl" => ' "" ',
},
"input" => {
},
"sequence" => {
"perl" => '" -sequence=$value -sformat=fasta"',
},
"required" => {
},
"sitelen" => {
"perl" => '" -sitelen=$value"',
},
"enzymes" => {
"perl" => '" -enzymes=$value"',
},
"advanced" => {
},
"min" => {
"perl" => '(defined $value && $value != $vdef)? " -min=$value" : ""',
},
"max" => {
"perl" => '(defined $value && $value != $vdef)? " -max=$value" : ""',
},
"single" => {
"perl" => '($value)? " -single" : ""',
},
"blunt" => {
"perl" => '($value)? "" : " -noblunt"',
},
"sticky" => {
"perl" => '($value)? "" : " -nosticky"',
},
"ambiguity" => {
"perl" => '($value)? "" : " -noambiguity"',
},
"plasmid" => {
"perl" => '($value)? " -plasmid" : ""',
},
"commercial" => {
"perl" => '($value)? "" : " -nocommercial"',
},
"datafile" => {
"perl" => '($value)? " -datafile=$value" : ""',
},
"output" => {
},
"limit" => {
"perl" => '($value)? "" : " -nolimit"',
},
"preferred" => {
"perl" => '($value)? " -preferred" : ""',
},
"alphabetic" => {
"perl" => '($value)? " -alphabetic" : ""',
},
"fragments" => {
"perl" => '($value)? " -fragments" : ""',
},
"name" => {
"perl" => '($value)? " -name" : ""',
},
"outfile" => {
"perl" => '" -outfile=$value"',
},
"auto" => {
"perl" => '" -auto -stdout"',
},
"restrict" => {
"perl" => '"restrict"',
}
};
$self->{FILENAMES} = {
};
$self->{SEQFMT} = {
"sequence" => [8],
};
$self->{GROUP} = {
"init" => -10,
"sequence" => 1,
"sitelen" => 2,
"enzymes" => 3,
"min" => 4,
"max" => 5,
"single" => 6,
"blunt" => 7,
"sticky" => 8,
"ambiguity" => 9,
"plasmid" => 10,
"commercial" => 11,
"datafile" => 12,
"limit" => 13,
"preferred" => 14,
"alphabetic" => 15,
"fragments" => 16,
"name" => 17,
"outfile" => 18,
"auto" => 19,
"restrict" => 0
};
$self->{BY_GROUP_PARAMETERS} = [
"init",
"input",
"required",
"advanced",
"output",
"restrict",
"sequence",
"sitelen",
"enzymes",
"min",
"max",
"single",
"blunt",
"sticky",
"ambiguity",
"plasmid",
"commercial",
"datafile",
"limit",
"preferred",
"alphabetic",
"fragments",
"name",
"outfile",
"auto",
];
$self->{SIZE} = {
};
$self->{ISHIDDEN} = {
"init" => 1,
"input" => 0,
"sequence" => 0,
"required" => 0,
"sitelen" => 0,
"enzymes" => 0,
"advanced" => 0,
"min" => 0,
"max" => 0,
"single" => 0,
"blunt" => 0,
"sticky" => 0,
"ambiguity" => 0,
"plasmid" => 0,
"commercial" => 0,
"datafile" => 0,
"output" => 0,
"limit" => 0,
"preferred" => 0,
"alphabetic" => 0,
"fragments" => 0,
"name" => 0,
"outfile" => 0,
"auto" => 1,
"restrict" => 1
};
$self->{ISCOMMAND} = {
"init" => 0,
"input" => 0,
"sequence" => 0,
"required" => 0,
"sitelen" => 0,
"enzymes" => 0,
"advanced" => 0,
"min" => 0,
"max" => 0,
"single" => 0,
"blunt" => 0,
"sticky" => 0,
"ambiguity" => 0,
"plasmid" => 0,
"commercial" => 0,
"datafile" => 0,
"output" => 0,
"limit" => 0,
"preferred" => 0,
"alphabetic" => 0,
"fragments" => 0,
"name" => 0,
"outfile" => 0,
"auto" => 0,
};
$self->{ISMANDATORY} = {
"init" => 0,
"input" => 0,
"sequence" => 1,
"required" => 0,
"sitelen" => 1,
"enzymes" => 1,
"advanced" => 0,
"min" => 0,
"max" => 0,
"single" => 0,
"blunt" => 0,
"sticky" => 0,
"ambiguity" => 0,
"plasmid" => 0,
"commercial" => 0,
"datafile" => 0,
"output" => 0,
"limit" => 0,
"preferred" => 0,
"alphabetic" => 0,
"fragments" => 0,
"name" => 0,
"outfile" => 1,
"auto" => 0,
};
$self->{PROMPT} = {
"init" => "",
"input" => "input Section",
"sequence" => "sequence -- DNA [sequences] (-sequence)",
"required" => "required Section",
"sitelen" => "Minimum recognition site length (-sitelen)",
"enzymes" => "Comma separated enzyme list (-enzymes)",
"advanced" => "advanced Section",
"min" => "Minimum cuts per RE (-min)",
"max" => "Maximum cuts per RE (-max)",
"single" => "Force single site only cuts (-single)",
"blunt" => "Allow blunt end cutters (-blunt)",
"sticky" => "Allow sticky end cutters (-sticky)",
"ambiguity" => "Allow ambiguous matches (-ambiguity)",
"plasmid" => "Allow circular DNA (-plasmid)",
"commercial" => "Only enzymes with suppliers (-commercial)",
"datafile" => "Alternative RE data file (-datafile)",
"output" => "output Section",
"limit" => "Limits reports to one isoschizomer (-limit)",
"preferred" => "Report preferred isoschizomers (-preferred)",
"alphabetic" => "Sort output alphabetically (-alphabetic)",
"fragments" => "Show fragment lengths (-fragments)",
"name" => "Show sequence name (-name)",
"outfile" => "outfile (-outfile)",
"auto" => "",
};
$self->{ISSTANDOUT} = {
"init" => 0,
"input" => 0,
"sequence" => 0,
"required" => 0,
"sitelen" => 0,
"enzymes" => 0,
"advanced" => 0,
"min" => 0,
"max" => 0,
"single" => 0,
"blunt" => 0,
"sticky" => 0,
"ambiguity" => 0,
"plasmid" => 0,
"commercial" => 0,
"datafile" => 0,
"output" => 0,
"limit" => 0,
"preferred" => 0,
"alphabetic" => 0,
"fragments" => 0,
"name" => 0,
"outfile" => 0,
"auto" => 0,
};
$self->{VLIST} = {
"input" => ['sequence',],
"required" => ['sitelen','enzymes',],
"advanced" => ['min','max','single','blunt','sticky','ambiguity','plasmid','commercial','datafile',],
"output" => ['limit','preferred','alphabetic','fragments','name','outfile',],
};
$self->{FLIST} = {
};
$self->{SEPARATOR} = {
};
$self->{VDEF} = {
"sitelen" => '4',
"enzymes" => 'all',
"min" => '1',
"max" => '2000000000',
"single" => '0',
"blunt" => '1',
"sticky" => '1',
"ambiguity" => '1',
"plasmid" => '0',
"commercial" => '1',
"limit" => '1',
"preferred" => '0',
"alphabetic" => '0',
"fragments" => '0',
"name" => '0',
"outfile" => 'outfile.out',
};
$self->{PRECOND} = {
"init" => { "perl" => '1' },
"input" => { "perl" => '1' },
"sequence" => { "perl" => '1' },
"required" => { "perl" => '1' },
"sitelen" => { "perl" => '1' },
"enzymes" => { "perl" => '1' },
"advanced" => { "perl" => '1' },
"min" => { "perl" => '1' },
"max" => { "perl" => '1' },
"single" => { "perl" => '1' },
"blunt" => { "perl" => '1' },
"sticky" => { "perl" => '1' },
"ambiguity" => { "perl" => '1' },
"plasmid" => { "perl" => '1' },
"commercial" => { "perl" => '1' },
"datafile" => { "perl" => '1' },
"output" => { "perl" => '1' },
"limit" => { "perl" => '1' },
"preferred" => { "perl" => '1' },
"alphabetic" => { "perl" => '1' },
"fragments" => { "perl" => '1' },
"name" => { "perl" => '1' },
"outfile" => { "perl" => '1' },
"auto" => { "perl" => '1' },
};
$self->{CTRL} = {
};
$self->{PIPEOUT} = {
};
$self->{WITHPIPEOUT} = {
};
$self->{PIPEIN} = {
"sequence" => {
"seqsfile" => '1',
},
};
$self->{WITHPIPEIN} = {
};
$self->{ISCLEAN} = {
"init" => 0,
"input" => 0,
"sequence" => 0,
"required" => 0,
"sitelen" => 0,
"enzymes" => 0,
"advanced" => 0,
"min" => 0,
"max" => 0,
"single" => 0,
"blunt" => 0,
"sticky" => 0,
"ambiguity" => 0,
"plasmid" => 0,
"commercial" => 0,
"datafile" => 0,
"output" => 0,
"limit" => 0,
"preferred" => 0,
"alphabetic" => 0,
"fragments" => 0,
"name" => 0,
"outfile" => 0,
"auto" => 0,
};
$self->{ISSIMPLE} = {
"init" => 0,
"input" => 0,
"sequence" => 1,
"required" => 0,
"sitelen" => 1,
"enzymes" => 1,
"advanced" => 0,
"min" => 0,
"max" => 0,
"single" => 0,
"blunt" => 0,
"sticky" => 0,
"ambiguity" => 0,
"plasmid" => 0,
"commercial" => 0,
"datafile" => 0,
"output" => 0,
"limit" => 0,
"preferred" => 0,
"alphabetic" => 0,
"fragments" => 0,
"name" => 0,
"outfile" => 1,
"auto" => 0,
};
$self->{PARAMFILE} = {
};
$self->{COMMENT} = {
"enzymes" => [
"The name \'all\' reads in all enzyme names from the REBASE database. You can specify enzymes by giving their names with commas between then, such as: \'HincII,hinfI,ppiI,hindiii\'. <BR> The case of the names is not important. You can specify a file of enzyme names to read in by giving the name of the file holding the enzyme names with a \'\@\' character in front of it, for example, \'\@enz.list\'. <BR> Blank lines and lines starting with a hash character or \'!\' are ignored and all other lines are concatenated together with a comma character \',\' and then treated as the list of enzymes to search for. <BR> An example of a file of enzyme names is: <BR> ! my enzymes <BR> HincII, ppiII <BR> ! other enzymes <BR> hindiii <BR> HinfI <BR> PpiI",
],
};
$self->{SCALEMIN} = {
};
$self->{SCALEMAX} = {
};
$self->{SCALEINC} = {
};
$self->{INFO} = {
};
# -- end of definitions extracted from /local/gensoft/lib/Pise/5.a/PerlDef/restrict.pm
$self->_init_params(@params);
return $self;
}
1; # Needed to keep compiler happy