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#!/usr/bin/env python
"""Tests for Primer-based programs in the Emboss suite.
"""
# standard library
import sys
import os
import unittest
# local stuff
from Bio.Emboss import PrimerSearch, Primer3
class Primer3ParseTest(unittest.TestCase):
def setUp(self):
self.test_files = \
[os.path.join("Emboss", "bac_find.primer3"),
os.path.join("Emboss", "cds_forward.primer3"),
os.path.join("Emboss", "cds_reverse.primer3"),
os.path.join("Emboss", "short.primer3"),
os.path.join("Emboss", "internal_oligo.primer3")
]
def test_simple_parse(self):
"""Make sure that we can parse all primer3 files.
"""
for file in self.test_files:
h = open(file, "r")
Primer3.read(h)
h.close()
def test_indepth_regular_parse(self):
"""Make sure we get the data from normal primer3 files okay.
"""
regular_file = self.test_files[0]
h = open(regular_file, "r")
primer_info = Primer3.read(h)
h.close()
assert len(primer_info.primers) == 5, \
"Wrong number of primers: %s" % len(primer_info.primers)
assert primer_info.primers[1].forward_seq \
== "CCGGTTTCTCTGGTTGAAAA"
assert primer_info.primers[2].reverse_seq == \
"TCACATTCCCAAATGTAGATCG"
assert primer_info.primers[0].size == 218
assert primer_info.primers[3].forward_start == 112
assert primer_info.primers[3].forward_length == 20
assert primer_info.primers[3].forward_tm == 59.57
assert primer_info.primers[3].forward_gc == 45.00
assert primer_info.primers[4].reverse_start == 304
assert primer_info.primers[4].reverse_length == 22
assert primer_info.primers[4].reverse_tm == 59.61
assert primer_info.primers[4].reverse_gc == 40.91
def test_in_depth_single_parse(self):
"""Make sure we get info right from a single primer find.
"""
file = self.test_files[1]
h = open(file, "r")
primer_info = Primer3.read(h)
h.close()
assert len(primer_info.primers) == 5
assert primer_info.primers[1].reverse_seq == ""
assert primer_info.primers[3].forward_seq == "TGTGATTGCTTGAGCTGGAC"
assert primer_info.primers[3].forward_start == 253
def test_internal_oligo_single_parse(self):
''' Make sure we can parse an internal oligo file correctly '''
# these files are generated when designing hybridization probes.
file = self.test_files[4]
h = open(file, "r")
primer_info = Primer3.read(h)
h.close()
assert len(primer_info.primers) == 5
assert primer_info.primers[0].internal_length == 22
assert primer_info.primers[1].internal_seq == 'TTGCGCTTTAGTTTGAATTGAA'
assert primer_info.primers[2].internal_tm == 58.62
assert primer_info.primers[3].internal_start == 16
assert primer_info.primers[4].internal_gc == 35.00
class PrimersearchParseTest(unittest.TestCase):
def setUp(self):
self.test_files = \
[os.path.join("Emboss", "bac_find.psearch")]
def test_simple_parse(self):
"""Make sure that we can parse all primersearch files.
"""
for file in self.test_files:
h = open(file, "r")
PrimerSearch.read(h)
h.close()
def test_in_depth_normal_parse(self):
"""Make sure the output from a simple primersearch file is correct.
"""
file = self.test_files[0]
h = open(file, "r")
amp_info = PrimerSearch.read(h)
h.close()
assert len(amp_info.amplifiers.keys()) == 1
assert "Test" in amp_info.amplifiers.keys()
assert len(amp_info.amplifiers["Test"]) == 1
assert amp_info.amplifiers["Test"][0].length == 218
assert amp_info.amplifiers["Test"][0].hit_info == \
"AC074298 AC074298 \n" + \
"\tTelomere associated sequence for Arabidopsis thaliana " + \
"TEL1N from chromosome I, complete sequence.\n" + \
"\tCCGGTTTCTCTGGTTGAAAA hits forward strand at 114 with " + \
"0 mismatches\n" + \
"\tTCACATTCCCAAATGTAGATCG hits reverse strand at [114] with " + \
"0 mismatches"
class PrimerSearchInputTest(unittest.TestCase):
"""Test creating input files for primersearch.
"""
def setUp(self):
pass
def test_primer_representation(self):
"""Make sure we can output primer information correctly.
"""
p_info = PrimerSearch.InputRecord()
p_info.add_primer_set("Test", "GATC", "CATG")
p_info.add_primer_set("Test2", "AATA", "TTAT")
output = str(p_info)
assert output == "Test GATC CATG\n" + \
"Test2 AATA TTAT\n"
if __name__ == "__main__":
runner = unittest.TextTestRunner(verbosity = 2)
unittest.main(testRunner=runner)
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