diff --git a/src/blockencodings.cpp b/src/blockencodings.cpp
index 4a311cbba2a6..65f0bf088f72 100644
--- a/src/blockencodings.cpp
+++ b/src/blockencodings.cpp
@@ -15,8 +15,6 @@
 
 #include <unordered_map>
 
-#define MIN_TRANSACTION_BASE_SIZE (::GetSerializeSize(CTransaction(), SER_NETWORK, PROTOCOL_VERSION | SERIALIZE_TRANSACTION_NO_WITNESS))
-
 CBlockHeaderAndShortTxIDs::CBlockHeaderAndShortTxIDs(const CBlock& block, bool fUseWTXID) :
         nonce(GetRand(std::numeric_limits<uint64_t>::max())),
         shorttxids(block.vtx.size() - 1), prefilledtxn(1), header(block) {
@@ -50,7 +48,7 @@ uint64_t CBlockHeaderAndShortTxIDs::GetShortID(const uint256& txhash) const {
 ReadStatus PartiallyDownloadedBlock::InitData(const CBlockHeaderAndShortTxIDs& cmpctblock, const std::vector<std::pair<uint256, CTransactionRef>>& extra_txn) {
     if (cmpctblock.header.IsNull() || (cmpctblock.shorttxids.empty() && cmpctblock.prefilledtxn.empty()))
         return READ_STATUS_INVALID;
-    if (cmpctblock.shorttxids.size() + cmpctblock.prefilledtxn.size() > MAX_BLOCK_BASE_SIZE / MIN_TRANSACTION_BASE_SIZE)
+    if (cmpctblock.shorttxids.size() + cmpctblock.prefilledtxn.size() > MAX_BLOCK_BASE_SIZE / MIN_TRANSACTION_SIZE)
         return READ_STATUS_INVALID;
 
     assert(header.IsNull() && txn_available.empty());
diff --git a/src/consensus/consensus.h b/src/consensus/consensus.h
index 351911a3a47b..c7c0a65f7e17 100644
--- a/src/consensus/consensus.h
+++ b/src/consensus/consensus.h
@@ -16,6 +16,8 @@ static const unsigned int MAX_BLOCK_WEIGHT = 4000000;
 static const unsigned int MAX_BLOCK_BASE_SIZE = 1000000;
 /** The maximum allowed number of signature check operations in a block (network rule) */
 static const int64_t MAX_BLOCK_SIGOPS_COST = 80000;
+/** Smallest possible transaction size */
+static const unsigned int MIN_TRANSACTION_SIZE = 60;
 /** Coinbase transaction outputs can only be spent after this number of new blocks (network rule) */
 static const int COINBASE_MATURITY = 100;
 
diff --git a/src/init.cpp b/src/init.cpp
index 5be011f944de..f0c1f2de6c84 100644
--- a/src/init.cpp
+++ b/src/init.cpp
@@ -693,6 +693,16 @@ bool InitSanityCheck(void)
     if (!glibc_sanity_test() || !glibcxx_sanity_test())
         return false;
 
+    CMutableTransaction tx;
+    tx.vin.resize(1);
+    tx.vout.resize(1);
+    size_t nMinTxSize = ::GetSerializeSize(tx, SER_NETWORK, PROTOCOL_VERSION);
+    size_t nMinStrippedTxSize = ::GetSerializeSize(tx, SER_NETWORK, PROTOCOL_VERSION | SERIALIZE_TRANSACTION_NO_WITNESS);
+    if (MIN_TRANSACTION_SIZE != nMinTxSize || MIN_TRANSACTION_SIZE != nMinStrippedTxSize) {
+        InitError(strprintf("MIN_TRANSACTION_SIZE verification failure: const %u vs min=%u vs minstripped=%u", MIN_TRANSACTION_SIZE, nMinTxSize, nMinStrippedTxSize));
+        return false;
+    }
+
     return true;
 }
 
diff --git a/src/merkleblock.cpp b/src/merkleblock.cpp
index 86c8172a31df..662bac8a0399 100644
--- a/src/merkleblock.cpp
+++ b/src/merkleblock.cpp
@@ -155,7 +155,7 @@ uint256 CPartialMerkleTree::ExtractMatches(std::vector<uint256> &vMatch, std::ve
     if (nTransactions == 0)
         return uint256();
     // check for excessively high numbers of transactions
-    if (nTransactions > MAX_BLOCK_BASE_SIZE / 60) // 60 is the lower bound for the size of a serialized CTransaction
+    if (nTransactions > MAX_BLOCK_BASE_SIZE / MIN_TRANSACTION_SIZE)
         return uint256();
     // there can never be more hashes provided than one for every txid
     if (vHash.size() > nTransactions)