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Merge pull request #1 from stevenweaver/master

Minor typo update
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lmoncla committed Apr 9, 2018
2 parents e6f1569 + c8e1abb commit 1caba1cbaf5cc09254b53d7bbb923a3ed6b9d2ca
Showing with 9 additions and 14 deletions.
  1. +9 −14 protocols/Library preparation.md
@@ -16,27 +16,22 @@ Library preparation was performed with the Nextera XT DNA Library Prep kit (Illu

6. To each tube, add 7.5 µl of NPM.

7. Being carefµl not to touch the caps, add 2.5 µl of the i5 indices to each tube.
7. Being careful not to touch the caps, add 2.5 µl of the i5 indices to each tube.

8. Add 2.5 µl of the i7 indices to each tube. Pipette up and down 5 times to mix.

9. Place tubes in a thermocycler and run the following limited-cycle PCR program.

Ensure that the thermocycler lid is heated during the incubation. (total volume = 25 µl)

72°C for 3 minutes

95°C for 30 seconds


12 cycles of:
95°C for 10 seconds
55°C for 30 seconds
72°C for 30 seconds


72°C for 5 minutes
Hold at 10°C
1. 72°C for 3 minutes
2. 95°C for 30 seconds
3. 12 cycles of:
1. 95°C for 10 seconds
2. 55°C for 30 seconds
3. 72°C for 30 seconds
4. 72°C for 5 minutes
5. Hold at 10°C

* After the PCR step is complete, reaction products can be stored at 4°C for up to 2 days.

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