diff --git a/chromium_src/third_party/blink/common/origin_trials/origin_trials.cc b/chromium_src/third_party/blink/common/origin_trials/origin_trials.cc
index 3358ae892bee6..8cbf2acceb0dd 100644
--- a/chromium_src/third_party/blink/common/origin_trials/origin_trials.cc
+++ b/chromium_src/third_party/blink/common/origin_trials/origin_trials.cc
@@ -36,7 +36,6 @@ bool IsTrialDisabledInBrave(std::string_view trial_name) {
       "SignedExchangeSubresourcePrefetch",
       "SubresourceWebBundles",
       "TrustTokens",
-      "WebEnvironmentIntegrity",
   };
 
   if (base::Contains(kBraveDisabledTrialNames, trial_name)) {
diff --git a/renderer/brave_content_renderer_client.cc b/renderer/brave_content_renderer_client.cc
index 27556bcb227cb..f6742a910a694 100644
--- a/renderer/brave_content_renderer_client.cc
+++ b/renderer/brave_content_renderer_client.cc
@@ -105,8 +105,6 @@ void BraveContentRendererClient::
   blink::WebRuntimeFeatures::EnableFeatureFromString(
       "SpeculationRulesPrefetchProxy", false);
   blink::WebRuntimeFeatures::EnableFeatureFromString("AdTagging", false);
-  blink::WebRuntimeFeatures::EnableFeatureFromString("WebEnvironmentIntegrity",
-                                                     false);
 }
 
 BraveContentRendererClient::~BraveContentRendererClient() = default;