The file cannot be accessed by the system

[captainsoccer]

hello i am a new user to this program, every time no matter what file i choose the same error shows up:
The file cannot be accessed by the system

[captainsoccer]

this is what happens:
log.txt

[brentvollebregt]

For searchability and others that want to see the error:

Running auto-py-to-exe v2.7.8
Building directory: C:\Users\Eyal\AppData\Local\Temp\tmp8rjl49fz
Provided command: pyinstaller --noconfirm --onefile --windowed  "E:/downloads/iahte.py"
Recursion Limit is set to 5000
Executing: pyinstaller --noconfirm --onefile --windowed E:/downloads/iahte.py --distpath C:\Users\Eyal\AppData\Local\Temp\tmp8rjl49fz\application --workpath C:\Users\Eyal\AppData\Local\Temp\tmp8rjl49fz\build --specpath C:\Users\Eyal\AppData\Local\Temp\tmp8rjl49fz

168210 INFO: PyInstaller: 4.1
168224 INFO: Python: 3.8.6
168239 INFO: Platform: Windows-10-10.0.19041-SP0
168243 INFO: wrote C:\Users\Eyal\AppData\Local\Temp\tmp8rjl49fz\iahte.spec
168250 INFO: UPX is not available.
168256 INFO: Extending PYTHONPATH with paths
['E:\\downloads', 'C:\\Users\\Eyal\\AppData\\Local\\Temp\\tmp8rjl49fz']
168271 INFO: checking Analysis
168286 INFO: Building Analysis because Analysis-01.toc is non existent
168302 INFO: Reusing cached module dependency graph...
168356 INFO: Caching module graph hooks...
168412 INFO: running Analysis Analysis-01.toc
An error occurred while packaging
Traceback (most recent call last):
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\win32ctypes\pywin32\pywintypes.py", line 35, in pywin32error
    yield
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\win32ctypes\pywin32\win32api.py", line 43, in LoadLibraryEx
    return _dll._LoadLibraryEx(fileName, 0, flags)
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\win32ctypes\core\cffi\_dll.py", line 23, in _LoadLibraryEx
    result = check_null(
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\win32ctypes\core\cffi\_util.py", line 81, in __call__
    self._raise_error(function_name)
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\win32ctypes\core\cffi\_util.py", line 92, in _raise_error
    raise exception
OSError: [WinError 1920] The file cannot be accessed by the system

During handling of the above exception, another exception occurred:

Traceback (most recent call last):
  File "C:\Users\Eyal\Desktop\auto-py-to-exe-master\auto_py_to_exe\packaging.py", line 131, in package
    run_pyinstaller()
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\PyInstaller\__main__.py", line 114, in run
    run_build(pyi_config, spec_file, **vars(args))
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\PyInstaller\__main__.py", line 65, in run_build
    PyInstaller.building.build_main.main(pyi_config, spec_file, **kwargs)
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\PyInstaller\building\build_main.py", line 720, in main
    build(specfile, kw.get('distpath'), kw.get('workpath'), kw.get('clean_build'))
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\PyInstaller\building\build_main.py", line 667, in build
    exec(code, spec_namespace)
  File "C:\Users\Eyal\AppData\Local\Temp\tmp8rjl49fz\iahte.spec", line 6, in <module>
    a = Analysis(['E:/downloads/iahte.py'],
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\PyInstaller\building\build_main.py", line 242, in __init__
    self.__postinit__()
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\PyInstaller\building\datastruct.py", line 160, in __postinit__
    self.assemble()
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\PyInstaller\building\build_main.py", line 390, in assemble
    self.binaries.extend(bindepend.Dependencies([('', python, '')],
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\PyInstaller\depend\bindepend.py", line 224, in Dependencies
    for ftocnm, fn in getAssemblyFiles(pth, manifest, redirects):
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\PyInstaller\depend\bindepend.py", line 403, in getAssemblyFiles
    for assembly in getAssemblies(pth):
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\PyInstaller\depend\bindepend.py", line 354, in getAssemblies
    res = GetManifestResources(pth)
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\PyInstaller\utils\win32\winmanifest.py", line 1000, in GetManifestResources
    return winresource.GetResources(filename, [RT_MANIFEST], names, languages)
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\PyInstaller\utils\win32\winresource.py", line 170, in GetResources
    hsrc = win32api.LoadLibraryEx(filename, 0, LOAD_LIBRARY_AS_DATAFILE)
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\win32ctypes\pywin32\win32api.py", line 43, in LoadLibraryEx
    return _dll._LoadLibraryEx(fileName, 0, flags)
  File "C:\Program Files\WindowsApps\PythonSoftwareFoundation.Python.3.8_3.8.1776.0_x64__qbz5n2kfra8p0\lib\contextlib.py", line 131, in __exit__
    self.gen.throw(type, value, traceback)
  File "C:\Users\Eyal\AppData\Local\Packages\PythonSoftwareFoundation.Python.3.8_qbz5n2kfra8p0\LocalCache\local-packages\Python38\site-packages\win32ctypes\pywin32\pywintypes.py", line 37, in pywin32error
    raise error(exception.winerror, exception.function, exception.strerror)
win32ctypes.pywin32.pywintypes.error: (1920, 'LoadLibraryEx', 'The file cannot be accessed by the system')

Project output will not be moved to output folder
Complete.

[brentvollebregt]

This looks like a pyinstaller error. Search for "win32ctypes.pywin32.pywintypes.error: (1920, 'LoadLibraryEx', 'The file cannot be accessed by the system')" in Google lead me to this comment on pyinstaller/pyinstaller#4941:

We don't support using python from the windows store when not using a virtual environment. Please use a virtual environment; that should fix this.