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Updated rST

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1 parent e725295 commit 3fe1343f45c009a7d49bb0782e9aa069fbba01b1 @buske committed Nov 9, 2014
Showing with 11 additions and 9 deletions.
  1. +11 −9 README.md
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@@ -10,24 +10,26 @@ Pipelines for analyzing padlock-captured DNA sequencing data
#### Install necessary scripts (bowtie, bismark, trimmomatic):
- `$ make install`
+ make install
#### Reference preparation:
- * Download fasta files for reference genome into subdirectory of `sequences` folder (or symlink them)
+* Download fasta files for reference genome into subdirectory of `sequences` folder (or symlink them), e.g.:
- e.g. `$ mkdir sequences/hg19; cd sequences/hg19; ln -s /path/to/hg19.fa`
+ mkdir sequences/hg19
+ cd sequences/hg19
+ ln -s /path/to/hg19.fa
- * Prepare bismark genomic indexes:
+* Prepare bismark genomic indexes:
- `$ lib/bismark_v0.12.3/bismark_genome_preparation sequences/phiX`
- `$ lib/bismark_v0.12.3/bismark_genome_preparation sequences/hg19`
+ lib/bismark_v0.12.3/bismark_genome_preparation sequences/phiX
+ lib/bismark_v0.12.3/bismark_genome_preparation sequences/hg19
### 2. Run pipeline on a sample directory
- Sample directory should contain paired-end files of the form: `<SAMPLE>_L001_R1_001.fastq.gz`, `<SAMPLE>_L001_R2_001.fastq.gz`)
+Sample directory should contain paired-end files of the form: `<SAMPLE>_L001_R1_001.fastq.gz`, `<SAMPLE>_L001_R2_001.fastq.gz`)
- Pipeline can then be run with SGE-compatible command:
+Pipeline can then be run with SGE-compatible command:
- `$ src/bis_seq/run_sample.sh hg19 /path/to/sample/`
+ src/bis_seq/run_sample.sh hg19 /path/to/sample/

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