diff --git a/README.md b/README.md index d9246c1..19ad8b8 100644 --- a/README.md +++ b/README.md @@ -10,24 +10,26 @@ Pipelines for analyzing padlock-captured DNA sequencing data #### Install necessary scripts (bowtie, bismark, trimmomatic): - `$ make install` + make install #### Reference preparation: - * Download fasta files for reference genome into subdirectory of `sequences` folder (or symlink them) +* Download fasta files for reference genome into subdirectory of `sequences` folder (or symlink them), e.g.: - e.g. `$ mkdir sequences/hg19; cd sequences/hg19; ln -s /path/to/hg19.fa` + mkdir sequences/hg19 + cd sequences/hg19 + ln -s /path/to/hg19.fa - * Prepare bismark genomic indexes: +* Prepare bismark genomic indexes: - `$ lib/bismark_v0.12.3/bismark_genome_preparation sequences/phiX` - `$ lib/bismark_v0.12.3/bismark_genome_preparation sequences/hg19` + lib/bismark_v0.12.3/bismark_genome_preparation sequences/phiX + lib/bismark_v0.12.3/bismark_genome_preparation sequences/hg19 ### 2. Run pipeline on a sample directory - Sample directory should contain paired-end files of the form: `_L001_R1_001.fastq.gz`, `_L001_R2_001.fastq.gz`) +Sample directory should contain paired-end files of the form: `_L001_R1_001.fastq.gz`, `_L001_R2_001.fastq.gz`) - Pipeline can then be run with SGE-compatible command: +Pipeline can then be run with SGE-compatible command: - `$ src/bis_seq/run_sample.sh hg19 /path/to/sample/` + src/bis_seq/run_sample.sh hg19 /path/to/sample/