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Updated rST

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1 parent 5a2d510 commit e869d19688ee99dde44e0d79bcf464533ed217eb @buske committed Nov 8, 2014
Showing with 9 additions and 9 deletions.
  1. +9 −9 README.md
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@@ -5,26 +5,26 @@ Pipelines for analyzing padlock-captured DNA sequencing data
-1) Initial configuration:
+## 1. Initial configuration:
-a) Install necessary scripts (bowtie, bismark, trimmomatic):
+### - Install necessary scripts (bowtie, bismark, trimmomatic):
`$ make install`
-b) Reference preparation:
+### - Reference preparation:
-- Download fasta files for reference genome into subdirectory of `sequences` folder (or symlink them)
+ * Download fasta files for reference genome into subdirectory of `sequences` folder (or symlink them)
- e.g. `$ mkdir sequences/hg19; cd sequences/hg19; ln -s /path/to/hg19.fa`
+ e.g. `$ mkdir sequences/hg19; cd sequences/hg19; ln -s /path/to/hg19.fa`
-- Prepare bismark genomic indexes:
+ * Prepare bismark genomic indexes:
- `$ lib/bismark_v0.12.3/bismark_genome_preparation sequences/phiX`
- `$ lib/bismark_v0.12.3/bismark_genome_preparation sequences/hg19`
+ `$ lib/bismark_v0.12.3/bismark_genome_preparation sequences/phiX`
+ `$ lib/bismark_v0.12.3/bismark_genome_preparation sequences/hg19`
-2) Run pipeline on a sample directory (containing paired-end files of the form: `<SAMPLE>_L001_R1_001.fastq.gz`, `<SAMPLE>_L001_R2_001.fastq.gz`):
+## 2. Run pipeline on a sample directory (containing paired-end files of the form: `<SAMPLE>_L001_R1_001.fastq.gz`, `<SAMPLE>_L001_R2_001.fastq.gz`):
`$ src/bis_seq/run_sample.sh hg19 /path/to/sample/`

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