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@@ -5,26 +5,26 @@ Pipelines for analyzing padlock-captured DNA sequencing data |
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-1) Initial configuration:
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+## 1. Initial configuration:
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-a) Install necessary scripts (bowtie, bismark, trimmomatic):
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+### - Install necessary scripts (bowtie, bismark, trimmomatic):
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`$ make install`
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-b) Reference preparation:
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+### - Reference preparation:
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-- Download fasta files for reference genome into subdirectory of `sequences` folder (or symlink them)
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+ * Download fasta files for reference genome into subdirectory of `sequences` folder (or symlink them)
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- e.g. `$ mkdir sequences/hg19; cd sequences/hg19; ln -s /path/to/hg19.fa`
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+ e.g. `$ mkdir sequences/hg19; cd sequences/hg19; ln -s /path/to/hg19.fa`
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-- Prepare bismark genomic indexes:
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+ * Prepare bismark genomic indexes:
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- `$ lib/bismark_v0.12.3/bismark_genome_preparation sequences/phiX`
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- `$ lib/bismark_v0.12.3/bismark_genome_preparation sequences/hg19`
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+ `$ lib/bismark_v0.12.3/bismark_genome_preparation sequences/phiX`
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+ `$ lib/bismark_v0.12.3/bismark_genome_preparation sequences/hg19`
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-2) Run pipeline on a sample directory (containing paired-end files of the form: `<SAMPLE>_L001_R1_001.fastq.gz`, `<SAMPLE>_L001_R2_001.fastq.gz`):
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+## 2. Run pipeline on a sample directory (containing paired-end files of the form: `<SAMPLE>_L001_R1_001.fastq.gz`, `<SAMPLE>_L001_R2_001.fastq.gz`):
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`$ src/bis_seq/run_sample.sh hg19 /path/to/sample/`
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