Parameter Value Version 1.5.8.3 User name antinori Machine name CD-6B9WJG2 Date of writing 05/09/2019 08:38:27 Fixed modifications Carbamidomethyl (C) Include contaminants True PSM FDR 0.01 Protein FDR 0.01 Site FDR 0.01 Use Normalized Ratios For Occupancy True Min. peptide Length 7 Min. score for unmodified peptides 0 Min. score for modified peptides 40 Min. delta score for unmodified peptides 0 Min. delta score for modified peptides 6 Min. unique peptides 1 Min. razor peptides 1 Min. peptides 2 Use only unmodified peptides and False Peptides used for protein quantification Unique Discard unmodified counterpart peptides True Label min. ratio count 1 Use delta score False iBAQ True iBAQ log fit True Match between runs True Matching time window [min] 2 Alignment time window [min] 20 Find dependent peptides False Fasta file E:\Data_MQ\Fasta_files\UP000005640_reviewed_9606_06052019.fasta Decoy mode revert Include contaminants True Advanced ratios False Fixed andromeda index folder Temporary folder Combined folder location E:\Data_MQ\PAM1816to1824_all Second peptides False Stabilize large LFQ ratios True Separate LFQ in parameter groups False Require MS/MS for LFQ comparisons True Calculate peak properties False Main search max. combinations 200 Advanced site intensities False Write msScans table True Write msmsScans table True Write ms3Scans table True Write allPeptides table True Write mzRange table True Max. peptide mass [Da] 4600 Min. peptide length for unspecific search 8 Max. peptide length for unspecific search 25 Razor protein FDR True Disable MD5 False Max mods in site table 3 Match unidentified features True MS/MS tol. (FTMS) 20 ppm Top MS/MS peaks per Da interval. (FTMS) 12 Da interval. (FTMS) 100 MS/MS deisotoping (FTMS) True MS/MS deisotoping tolerance (FTMS) 7 MS/MS deisotoping tolerance unit (FTMS) ppm MS/MS higher charges (FTMS) True MS/MS water loss (FTMS) True MS/MS ammonia loss (FTMS) True MS/MS dependent losses (FTMS) True MS/MS recalibration (FTMS) False MS/MS tol. (ITMS) 0.5 Da Top MS/MS peaks per Da interval. (ITMS) 8 Da interval. (ITMS) 100 MS/MS deisotoping (ITMS) False MS/MS deisotoping tolerance (ITMS) 0.15 MS/MS deisotoping tolerance unit (ITMS) Da MS/MS higher charges (ITMS) True MS/MS water loss (ITMS) True MS/MS ammonia loss (ITMS) True MS/MS dependent losses (ITMS) True MS/MS recalibration (ITMS) False MS/MS tol. (TOF) 40 ppm Top MS/MS peaks per Da interval. (TOF) 10 Da interval. (TOF) 100 MS/MS deisotoping (TOF) True MS/MS deisotoping tolerance (TOF) 0.01 MS/MS deisotoping tolerance unit (TOF) Da MS/MS higher charges (TOF) True MS/MS water loss (TOF) True MS/MS ammonia loss (TOF) True MS/MS dependent losses (TOF) True MS/MS recalibration (TOF) False MS/MS tol. (Unknown) 0.5 Da Top MS/MS peaks per Da interval. (Unknown) 8 Da interval. (Unknown) 100 MS/MS deisotoping (Unknown) False MS/MS deisotoping tolerance (Unknown) 0.15 MS/MS deisotoping tolerance unit (Unknown) Da MS/MS higher charges (Unknown) True MS/MS water loss (Unknown) True MS/MS ammonia loss (Unknown) True MS/MS dependent losses (Unknown) True MS/MS recalibration (Unknown) False Site tables Oxidation (M)Sites.txt