Dockerfile

FROM stackbrew/ubuntu:13.10
MAINTAINER Brad Chapman "https://github.com/chapmanb"

# Setup a base system 
RUN apt-get update && apt-get install -y build-essential zlib1g-dev wget curl python-setuptools git

# Fake a fuse install; openjdk pulls this in 
# https://github.com/dotcloud/docker/issues/514
# https://gist.github.com/henrik-muehe/6155333
RUN mkdir -p /tmp/fuse-hack && cd /tmp/fuse-hack && \
    apt-get install libfuse2 && \
    apt-get download fuse && \
    dpkg-deb -x fuse_* . && \
    dpkg-deb -e fuse_* && \
    rm fuse_*.deb && \
    echo -en '#!/bin/bash\nexit 0\n' > DEBIAN/postinst && \
    dpkg-deb -b . /fuse.deb && \
    dpkg -i /fuse.deb && \
    rm -rf /tmp/fuse-hack

# bcbio-nextgen installation
RUN mkdir -p /tmp/bcbio-nextgen-install && cd /tmp/bcbio-nextgen-install && \
    wget --no-check-certificate \
      https://raw.github.com/chapmanb/bcbio-nextgen/master/scripts/bcbio_nextgen_install.py && \
    python bcbio_nextgen_install.py /usr/local/share/bcbio-nextgen --tooldir=/usr/local \
      --toolplus data --nodata --nosudo -u development && \
    bcbio_nextgen.py upgrade --isolate -u development && \
    echo 'export PATH=/usr/local/bin:$PATH' >> /etc/profile && \
    echo 'export LD_LIBRARY_PATH=/usr/local/lib:$LD_LIBRARY_PATH' >> /etc/profile && \
    echo 'export PERL5LIB=/usr/local/lib/perl5:/usr/local/lib/perl5/site_perl:${PERL5LIB}' >> /etc/profile && \
    apt-get clean && \
    rm -rf /var/lib/apt/lists/* /var/tmp/* && \
    /usr/local/share/bcbio-nextgen/anaconda/bin/conda remove --yes qt && \
    /usr/local/share/bcbio-nextgen/anaconda/bin/conda clean --yes --tarballs && \
    rm -rf /usr/local/share/bcbio-nextgen/anaconda/pkgs/qt* && \
    rm -rf $(brew --cache) && \
    rm -rf /.cpanm && \
    rm -rf /tmp/bcbio-nextgen-install

# Create directories and symlinks for data 
RUN mkdir -p /mnt/biodata && \
    mkdir -p /tmp/bcbio-nextgen && \
    mv /usr/local/share/bcbio-nextgen/galaxy/bcbio_system.yaml /usr/local/share/bcbio-nextgen/config && \
    rmdir /usr/local/share/bcbio-nextgen/galaxy && \
    ln -s /mnt/biodata/galaxy /usr/local/share/bcbio-nextgen/galaxy && \
    ln -s /mnt/biodata/gemini_data /usr/local/share/bcbio-nextgen/gemini_data && \
    ln -s /mnt/biodata/genomes /usr/local/share/bcbio-nextgen/genomes && \
    ln -s /mnt/biodata/liftOver /usr/local/share/bcbio-nextgen/liftOver && \
    chmod a+rwx /usr/local/share/bcbio-nextgen && \
    chmod a+rwx /usr/local/share/bcbio-nextgen/config && \
    chmod a+rwx /usr/local/share/bcbio-nextgen/config/*.yaml && \
    chmod a+rwx /usr/local/share/bcbio-nextgen/gemini-config.yaml

# Ensure permissions are set for update in place by arbitrary users
RUN find /usr/local -perm /u+x -execdir chmod a+x {} \;
RUN find /usr/local -perm /u+w -execdir chmod a+w {} \;

EXPOSE 8085
ENTRYPOINT ["/usr/local/bin/bcbio_nextgen.py", "server", "--port", "8085", "--biodata_dir", "/mnt/biodata"]