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Translates coordinates on circular microscopy dish in separate recordings sessions based on two reference coordinates.
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README.md

Recording location coordinate converter

Translates coordinates on circular microscopy dish in separate recording sessions based on two reference marks on the dish surface. Assumes that one of the reference marks is set to zero in both first and second sessions. Uses the transform of the second reference mark to calculate the transform of other locations on the dish and the dish rotation, to allow recordings in same locations.

Written for use with software on microscopes of FILM microscopy facility at Imperial College London, but should work for any microscopy software that allows setting a coordinate system based on a reference point.

Binder

Procedure

  1. Set zero location on first reference mark.
  2. Note coordinate of second reference mark.
  3. Make recordings as per experiment being conducted, noting coordinates of each recording location.
  4. In second recording session (after dish may have been removed to stain for different fluorescent marker), start by again setting zero location to first reference mark.
  5. Note coordinate of second reference mark in this second session.
  6. Open binder link above (and below) and input coordinate data into Jupyter notebook cell to calculate converter coordinates to return to the same location.

Binder

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