From 01a3aa73280ad1e057b89c1c18feab50e0fb34fb Mon Sep 17 00:00:00 2001 From: MehtapIsik Date: Mon, 8 Aug 2016 14:50:51 -0400 Subject: [PATCH] Add HSA competition assay data of 2015/10/12. --- .../MI_FLU_hsa_lig1_20151117_110602.xml | 1012 ++++++++++++++++ ..._lig1_pb_20151113 2015-11-13 1350.DATA.xml | 1065 +++++++++++++++++ data/hsa_competition_assay_example/README.md | 35 + .../ligand_DMSO_stocks.csv | 1 + 4 files changed, 2113 insertions(+) create mode 100644 data/hsa_competition_assay_example/MI_FLU_hsa_lig1_20151117_110602.xml create mode 100644 data/hsa_competition_assay_example/MI_FLU_hsa_lig1_pb_20151113 2015-11-13 1350.DATA.xml create mode 100644 data/hsa_competition_assay_example/README.md create mode 100644 data/hsa_competition_assay_example/ligand_DMSO_stocks.csv diff --git a/data/hsa_competition_assay_example/MI_FLU_hsa_lig1_20151117_110602.xml b/data/hsa_competition_assay_example/MI_FLU_hsa_lig1_20151117_110602.xml new file mode 100644 index 0000000..e4fce1e --- /dev/null +++ b/data/hsa_competition_assay_example/MI_FLU_hsa_lig1_20151117_110602.xml @@ -0,0 +1,1012 @@ + + + + + + + dansylamide + + +
+ + + + + + infinite M1000 PRO + 1311007572 + + + MEX, V 1.20 Safire2 MCR (V 1.20 Safire2 MCR ) + MEM, V 1.20 Safire2 MCR (V 1.20 Safire2 MCR ) + FPO, V_1.03_Safire2 FP (V_1.03_Safire2 FP ) + ABS, V 1.00 MCR Abs 4 Channel (V 1.00 MCR Abs 4 Channel) + LUM, V_1.04_11/2011_LUMINESCENCE (Nov 02 2011/17.53.34) + BCR, V_1.00_02/2008_S3FTCAN + INA, V_1.05_11/2011_S3LCE_ALPHA (Nov 3 2011/09.27.24) + INB, V_1.05_11/2011_S3LCE_ALPHA (Nov 3 2011/09.27.24) + TCAN, V_1.00_02/2008_S3FTCAN (Feb 21 2008/17.19.16) + + + + + + +
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+ \ No newline at end of file diff --git a/data/hsa_competition_assay_example/MI_FLU_hsa_lig1_pb_20151113 2015-11-13 1350.DATA.xml b/data/hsa_competition_assay_example/MI_FLU_hsa_lig1_pb_20151113 2015-11-13 1350.DATA.xml new file mode 100644 index 0000000..70e8e2a --- /dev/null +++ b/data/hsa_competition_assay_example/MI_FLU_hsa_lig1_pb_20151113 2015-11-13 1350.DATA.xml @@ -0,0 +1,1065 @@ + + + 3.0.2 + 2 + C:\Users\Thermo\Documents\HP D300\Protocols\MI_FLU_hsa_lig1_pb_20151113.hpdd + True + 2015-11-13T13:48:41.070-05:00 + 2015-11-13T13:50:12.355-05:00 + LastPriority + + + dansylamide + 20 + mM + 0 + 0 + + + phenylbutazone + 20 + mM + 0 + 0 + + + indomethacin + 20 + mM + 0 + 0 + + + DMSO normalization + True + 0 + 0 + + + + + Default96 + 96 Default + plate + 8 + 12 + 100000 + 0.01 + 0.05 + + + + + T8 + t8 + + + + + 7651.72050380243 + 6392.24 + + + 7651.72050380243 + 6391.56 + + + 8580 + 7200 + + + 2000 + 1200 + + + 21809.1397480988 + 18704 + + + 21809.1397480988 + 18704 + + + 21809.1397480988 + 18704.8 + + + 21809.1397480988 + 18704 + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + \ No newline at end of file diff --git a/data/hsa_competition_assay_example/README.md b/data/hsa_competition_assay_example/README.md new file mode 100644 index 0000000..24f220b --- /dev/null +++ b/data/hsa_competition_assay_example/README.md @@ -0,0 +1,35 @@ +# 2015/10/12 HSA Fluorescent Competition Assay + +Performed by Mehtap Isik. +For lab notebook: https://drive.google.com/drive/u/0/folders/0BykO-ZGQb9DHMzBIRWE5NUFQbFU + +- Protein: Human Serum Albumin (HSA) +- Fluorescent probe ligand: Dansylamide +- Non-fluorescent ligand: Phenylbutazone or Indomethacin + +## Protein and Buffer Solutions +HSA stock solution concentration in this assay is 0.5 uM. It is prepared by dilution of 10.0 uM HSA solution (prepared on 2015/09/10). +Buffer: 100 mM sodium phosphate buffer, pH 7.4 (i.e. CB7 Host-Guest Buffer) + +## Plate Layout +Row A : HSA + Dansylamide titration +Row B : buffer + Dansylamide titration +Row C : HSA + Dansylamide titration + constant concentration of phenylbutazone (10 uM) +Row D : HSA + Dansylamide titration + constant concentration of phenylbutazone (10 uM) +Row E : buffer + Dansylamide titration + constant concentration of phenylbutazone (50 uM) +Row F : buffer + Dansylamide titration + constant concentration of phenylbutazone (50 uM) +Row G : HSA + Dansylamide titration + constant concentration of indomethacin (10 uM) +Row H : buffer + Dansylamide titration + constant concentration of indomethacin (50 uM) + +- Plate type: 4ti_0223 (96 well, black clear bottom) + +## Files +Infinite data file: +`MI_FLU_hsa_lig1_20151117_110602.xml` + +D300 simulation report: +`MI_FLU_hsa_lig1_pb_20151113 2015-11-13 1350.DATA.xml` - D300 simulation report for dispensing Dansyl amide, Phenylbutazone or Indomethacin. + +Compound stocks spreadsheet: +`ligand_DMSO_stocks.csv` - DAM001, PHE001 and IND001 DMSO stocks are used in this experiment. + diff --git a/data/hsa_competition_assay_example/ligand_DMSO_stocks.csv b/data/hsa_competition_assay_example/ligand_DMSO_stocks.csv new file mode 100644 index 0000000..2aa3c5a --- /dev/null +++ b/data/hsa_competition_assay_example/ligand_DMSO_stocks.csv @@ -0,0 +1 @@ +id,compound name,supplier,product number,lot number,purity,molecular weight,compound mass (mg),solvent mass (g),notes BOS001,bosutinib,Sigma-Aldrich,ABCDE,12345,0.97,530.446,10.1,12.8,Example DAM001,dansylamide,Acros,unknown,A0297999,0.99,250.31,50.245,10.9998,UUID: CXN6P7 tempID: D18 PHE001,phenylbutazone,Acros,AC199310050,A0267015,0.99,308.38,61.965,10.9997,UUID: 6J20FF tempID: D17 IND001,indomethacin,Sigma,I7378-5G,BCBK0293V,0.99,357.79,71.7,10.9992,UUID: 8K9MJ4 tempID: D20 \ No newline at end of file