diff --git a/cocos/scripting/js-bindings/manual/jsb_cocos2dx_manual.cpp b/cocos/scripting/js-bindings/manual/jsb_cocos2dx_manual.cpp
index f245dea1970..40f7c74572e 100644
--- a/cocos/scripting/js-bindings/manual/jsb_cocos2dx_manual.cpp
+++ b/cocos/scripting/js-bindings/manual/jsb_cocos2dx_manual.cpp
@@ -444,6 +444,12 @@ static bool js_CanvasRenderingContext2D_setCanvasBufferUpdatedCallback(se::State
                         se::ScriptEngine::getInstance()->clearException();
                     }
                 };
+                // Add an unroot to avoid the root of the copy constructor caused by the internal reference of Lambda.
+                if (jsThis.isObject())
+                {
+                    jsThis.toObject()->unroot();
+                }
+                jsFunc.toObject()->unroot();
                 arg0 = lambda;
             }
             else