diff --git a/.changeset/yellow-paws-search.md b/.changeset/yellow-paws-search.md
new file mode 100644
index 000000000..9c4d58b35
--- /dev/null
+++ b/.changeset/yellow-paws-search.md
@@ -0,0 +1,6 @@
+---
+'@crxjs/vite-plugin': patch
+---
+
+Vite 5 moved vite manifest from 'manifest.json' to '.vite/manifest.json'. 
+This change updates the plugin to use the new location if Vite major version is >4, old location otherwise.
\ No newline at end of file
diff --git a/packages/vite-plugin/src/node/plugin-webAccessibleResources.ts b/packages/vite-plugin/src/node/plugin-webAccessibleResources.ts
index 3a1311145..3306d8ce2 100644
--- a/packages/vite-plugin/src/node/plugin-webAccessibleResources.ts
+++ b/packages/vite-plugin/src/node/plugin-webAccessibleResources.ts
@@ -1,5 +1,9 @@
 import { OutputChunk } from 'rollup'
-import { Manifest as ViteManifest, ResolvedConfig } from 'vite'
+import {
+  Manifest as ViteManifest,
+  ResolvedConfig,
+  version as ViteVersion,
+} from 'vite'
 import { compileFileResources } from './compileFileResources'
 import { contentScripts } from './contentScripts'
 import { DYNAMIC_RESOURCE } from './defineManifest'
@@ -102,10 +106,16 @@ export const pluginWebAccessibleResources: CrxPluginFn = () => {
 
         // derive content script resources from vite file manifest
         if (contentScripts.size > 0) {
+          // Vite 5 changed the manifest.json location to .vite/manifest.json.
+          // In order to support both Vite <=4 and Vite 5, we need to check the Vite version and determine the path accordingly.
+          const viteMajorVersion = parseInt(ViteVersion.split('.')[0])
+          const manifestPath = viteMajorVersion > 4 ? '.vite/manifest.json' : 'manifest.json'
+
           const viteManifest = parseJsonAsset<ViteManifest>(
             bundle,
-            'manifest.json',
+            manifestPath,
           )
+
           const viteFiles = new Map()
           for (const [, file] of Object.entries(viteManifest))
             viteFiles.set(file.file, file)