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Tools for automated design of CRISPR guides for DASH, FLASH and CRISPR tagging experiments.
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README.org

guide_design_tools: Automated design of Cas9 guide RNAs

guide_design_tools is a collection of software for the automated design of Cas9 guide RNAs (gRNAs):

  1. DASHit designs gRNAs for DASH experiments based either on a set of existing sequencing reads, or based on an explicitly provided target sequence.

Installation

DASHit runs on Linux and macOS. Installing DASHit requires:

  1. g++
  2. go version 1.9 or later
  3. python3

To install:

git clone https://github.com/czbiohub/guide_design_tools
cd guide_design_tools
make install

License

For academic and non-profit uses, guide_design_tools is free to use and modify. You must read and agree to the LICENSE.md before using guide_design_tools.

If you are intersted in using guide_design_tools for commercial purposes, please contact Steve Collier at the CZ Biohub.

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