STAR --genomeDir /mnt/d/NGS/rna-seq/03_ref --outSAMunmapped Within --outFilterType BySJout --outSAMattributes NH HI AS NM MD --outFilterMultimapNmax 20 --outFilterMismatchNmax 999 --outFilterMismatchNoverLmax 0.04 --alignIntronMin 20 --alignIntronMax 1000000 --alignMatesGapMax 1000000 --alignSJoverhangMin 8 --alignSJDBoverhangMin 1 --sjdbScore 1 --runThreadN 6 --genomeLoad NoSharedMemory --outSAMtype BAM Unsorted --quantMode TranscriptomeSAM --outSAMheaderHD @HD VN:1.4 SO:unsorted --outFileNamePrefix sub1-Ca13mCh-LGC9389_L1_PE_quals.temp/sub1-Ca13mCh-LGC9389_L1_PE_quals --readFilesIn sub1-Ca13mCh-LGC9389_L1_1_cleaned.fq sub1-Ca13mCh-LGC9389_L1_2_cleaned.fq Apr 26 10:56:19 ..... started STAR run Apr 26 10:56:19 ..... loading genome EXITING because of FATAL ERROR: could not open genome file /mnt/d/NGS/rna-seq/03_ref/genomeParameters.txt SOLUTION: check that the path to genome files, specified in --genomeDir is correct and the files are present, and have user read permsissions Apr 26 10:56:19 ...... FATAL ERROR, exiting "STAR --genomeDir /mnt/d/NGS/rna-seq/03_ref --outSAMunmapped Within --outFilterType BySJout --outSAMattributes NH HI AS NM MD --outFilterMultimapNmax 20 --outFilterMismatchNmax 999 --outFilterMismatchNoverLmax 0.04 --alignIntronMin 20 --alignIntronMax 1000000 --alignMatesGapMax 1000000 --alignSJoverhangMin 8 --alignSJDBoverhangMin 1 --sjdbScore 1 --runThreadN 6 --genomeLoad NoSharedMemory --outSAMtype BAM Unsorted --quantMode TranscriptomeSAM --outSAMheaderHD @HD VN:1.4 SO:unsorted --outFileNamePrefix sub1-Ca13mCh-LGC9389_L1_PE_quals.temp/sub1-Ca13mCh-LGC9389_L1_PE_quals --readFilesIn sub1-Ca13mCh-LGC9389_L1_1_cleaned.fq sub1-Ca13mCh-LGC9389_L1_2_cleaned.fq" failed! Plase check if you provide correct parameters/options for the pipeline!