Standard method for running 96 PACE experiments on one plate
Parameters
- Lagoon volume: 500uL
- Cycle time: 30 minutes
Steps
- Adds an on-deck inducer reagent
- 1000uL channels stage tips to be picked up by 96-channel head
- Implements nearly-continuous culture replacement in 96 lagoons
- Samples lagoons into microplate at intervals
- Coordinates with 3rd party plate reader to take luminescence and absorbance measurements
- Automatically bleaches and rinses culture reservoir
dgretton@mit.edu (maintainer)