diff --git a/.github/workflows/test.yml b/.github/workflows/test.yml
index f3605a420..8b3c6301d 100644
--- a/.github/workflows/test.yml
+++ b/.github/workflows/test.yml
@@ -21,7 +21,7 @@ jobs:
       fail-fast: false
       max-parallel: 5
       matrix:
-        python-version: ['3.9', '3.10', '3.11', '3.12', '3.13', '3.14']
+        python-version: ['3.10', '3.11', '3.12', '3.13', '3.14']
 
     services:
       mariadb:
@@ -91,11 +91,9 @@ jobs:
       fail-fast: false
       max-parallel: 5
       matrix:
-        python-version: ['3.9', '3.10', '3.11', '3.12', '3.13', '3.14']
+        python-version: ['3.10', '3.11', '3.12', '3.13', '3.14']
         database: [postgresql, postgis, psycopg3]
         exclude:
-            - python-version: '3.9'
-              database: psycopg3
             - python-version: '3.13'
               database: postgis
             - python-version: '3.13'
@@ -192,7 +190,7 @@ jobs:
       fail-fast: false
       max-parallel: 5
       matrix:
-        python-version: ['3.9', '3.10', '3.11', '3.12', '3.13', '3.14']
+        python-version: ['3.10', '3.11', '3.12', '3.13', '3.14']
 
     steps:
     - uses: actions/checkout@v5
@@ -252,7 +250,7 @@ jobs:
     - name: Set up Python ${{ matrix.python-version }}
       uses: actions/setup-python@v6
       with:
-        python-version: 3.9
+        python-version: '3.10'
 
     - name: Get pip cache dir
       id: pip-cache
diff --git a/debug_toolbar/_stubs.py b/debug_toolbar/_stubs.py
index dee6280d0..07217f39d 100644
--- a/debug_toolbar/_stubs.py
+++ b/debug_toolbar/_stubs.py
@@ -1,6 +1,6 @@
 from __future__ import annotations
 
-from typing import Any, NamedTuple, Optional, Protocol
+from typing import Any, NamedTuple, Protocol
 
 from django import template as dj_template
 from django.http import HttpRequest, HttpResponse
@@ -15,7 +15,7 @@ class InspectStack(NamedTuple):
     index: int
 
 
-TidyStackTrace = list[tuple[str, int, str, str, Optional[Any]]]
+TidyStackTrace = list[tuple[str, int, str, str, Any | None]]
 
 
 class RenderContext(dj_template.context.RenderContext):
diff --git a/debug_toolbar/middleware.py b/debug_toolbar/middleware.py
index 22d345a3f..862a38f9e 100644
--- a/debug_toolbar/middleware.py
+++ b/debug_toolbar/middleware.py
@@ -4,8 +4,8 @@
 
 import re
 import socket
+from collections.abc import Callable
 from functools import cache
-from typing import Callable
 
 from asgiref.sync import (
     async_to_sync,
diff --git a/debug_toolbar/toolbar.py b/debug_toolbar/toolbar.py
index 929ce7b70..ec0472790 100644
--- a/debug_toolbar/toolbar.py
+++ b/debug_toolbar/toolbar.py
@@ -7,8 +7,8 @@
 import logging
 import re
 import uuid
+from collections.abc import Callable
 from functools import cache
-from typing import Callable
 
 from django.apps import apps
 from django.conf import settings
diff --git a/docs/changes.rst b/docs/changes.rst
index 705611c82..4cfa9e7ca 100644
--- a/docs/changes.rst
+++ b/docs/changes.rst
@@ -11,6 +11,7 @@ Pending
   resources.
 * Show the cache backend alias and cache backend class name instead of
   the cache instance in the cache panel.
+* Dropped support for the Python 3.9, it has reached its end of life date.
 
 6.1.0 (2025-10-30)
 ------------------
diff --git a/pyproject.toml b/pyproject.toml
index 28e921337..53bc9bcc5 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -12,7 +12,7 @@ license = { text = "BSD-3-Clause" }
 authors = [
   { name = "Rob Hudson" },
 ]
-requires-python = ">=3.9"
+requires-python = ">=3.10"
 classifiers = [
   "Development Status :: 5 - Production/Stable",
   "Environment :: Web Environment",
@@ -25,7 +25,6 @@ classifiers = [
   "Operating System :: OS Independent",
   "Programming Language :: Python",
   "Programming Language :: Python :: 3 :: Only",
-  "Programming Language :: Python :: 3.9",
   "Programming Language :: Python :: 3.10",
   "Programming Language :: Python :: 3.11",
   "Programming Language :: Python :: 3.12",
@@ -57,7 +56,7 @@ packages = [
 path = "debug_toolbar/__init__.py"
 
 [tool.ruff]
-target-version = "py39"
+target-version = "py310"
 
 fix = true
 show-fixes = true
diff --git a/tests/base.py b/tests/base.py
index c18d3d1ed..152bc32e1 100644
--- a/tests/base.py
+++ b/tests/base.py
@@ -1,5 +1,4 @@
 import contextvars
-from typing import Optional
 
 import html5lib
 from asgiref.local import Local
@@ -70,7 +69,7 @@ class BaseMixin:
     client_class = ToolbarTestClient
     async_client_class = AsyncToolbarTestClient
 
-    panel: Optional[Panel] = None
+    panel: Panel | None = None
     panel_id = None
 
     def setUp(self):
diff --git a/tox.ini b/tox.ini
index c885f87a1..e7058e0fd 100644
--- a/tox.ini
+++ b/tox.ini
@@ -3,7 +3,6 @@ isolated_build = true
 envlist =
     docs
     packaging
-    py{39,310,311,312}-dj{42}-{sqlite,postgresql,postgis,mysql}
     py{310,311}-dj{42,51,52}-{sqlite,postgresql,psycopg3,postgis,mysql}
     py{312}-dj{42,51,52,60}-{sqlite,postgresql,psycopg3,postgis,mysql}
     py{313}-dj{51,52,60,main}-{sqlite,psycopg3,postgis3,mysql}
@@ -55,28 +54,28 @@ pip_pre = True
 commands = python -b -W always -m coverage run -m django test -v2 {posargs:tests}
 
 
-[testenv:py{39,310,311,312,313,314}-dj{42,51,52,60,main}-{postgresql,psycopg3}]
+[testenv:py{310,311,312,313,314}-dj{42,51,52,60,main}-{postgresql,psycopg3}]
 setenv =
     {[testenv]setenv}
     DB_BACKEND = postgresql
     DB_PORT = {env:DB_PORT:5432}
 
 
-[testenv:py{39,310,311,312,313,314}-dj{42,51,52,60,main}-{postgis,postgis3}]
+[testenv:py{310,311,312,313,314}-dj{42,51,52,60,main}-{postgis,postgis3}]
 setenv =
     {[testenv]setenv}
     DB_BACKEND = postgis
     DB_PORT = {env:DB_PORT:5432}
 
 
-[testenv:py{39,310,311,312,313,314}-dj{42,51,52,60,main}-mysql]
+[testenv:py{310,311,312,313,314}-dj{42,51,52,60,main}-mysql]
 setenv =
     {[testenv]setenv}
     DB_BACKEND = mysql
     DB_PORT = {env:DB_PORT:3306}
 
 
-[testenv:py{39,310,311,312,313,314}-dj{42,51,52,60,main}-sqlite]
+[testenv:py{310,311,312,313,314}-dj{42,51,52,60,main}-sqlite]
 setenv =
     {[testenv]setenv}
     DB_BACKEND = sqlite3
@@ -101,7 +100,6 @@ skip_install = true
 
 [gh-actions]
 python =
-    3.9: py39
     3.10: py310
     3.11: py311
     3.12: py312