diff --git a/django/contrib/contenttypes/fields.py b/django/contrib/contenttypes/fields.py
index 6c371557c58a5..85b39221288ad 100644
--- a/django/contrib/contenttypes/fields.py
+++ b/django/contrib/contenttypes/fields.py
@@ -1,3 +1,4 @@
+import itertools
 from collections import defaultdict
 
 from django.contrib.contenttypes.models import ContentType
@@ -568,19 +569,28 @@ def get_prefetch_queryset(self, instances, queryset=None):
 
             queryset._add_hints(instance=instances[0])
             queryset = queryset.using(queryset._db or self._db)
-
-            query = {
-                '%s__pk' % self.content_type_field_name: self.content_type.id,
-                '%s__in' % self.object_id_field_name: {obj.pk for obj in instances}
-            }
-
+            # Group instances by content types.
+            content_type_queries = iter(
+                models.Q(**{
+                    '%s__pk' % self.content_type_field_name: content_type_id,
+                    '%s__in' % self.object_id_field_name: {obj.pk for obj in objs}
+                })
+                for content_type_id, objs in
+                itertools.groupby(instances, lambda obj: self.get_content_type(obj).pk)
+            )
+            query = next(content_type_queries)
+            for q in content_type_queries:
+                query |= q
             # We (possibly) need to convert object IDs to the type of the
             # instances' PK in order to match up instances:
             object_id_converter = instances[0]._meta.pk.to_python
             return (
-                queryset.filter(**query),
-                lambda relobj: object_id_converter(getattr(relobj, self.object_id_field_name)),
-                lambda obj: obj.pk,
+                queryset.filter(query),
+                lambda relobj: (
+                    object_id_converter(getattr(relobj, self.object_id_field_name)),
+                    relobj.content_type_id
+                ),
+                lambda obj: (obj.pk, self.get_content_type(obj).pk),
                 False,
                 self.prefetch_cache_name,
                 False,
diff --git a/tests/generic_relations/tests.py b/tests/generic_relations/tests.py
index 2e99c5b5cf41a..7c0db95908999 100644
--- a/tests/generic_relations/tests.py
+++ b/tests/generic_relations/tests.py
@@ -546,6 +546,24 @@ def test_add_then_remove_after_prefetch(self):
         platypus.tags.remove(weird_tag)
         self.assertSequenceEqual(platypus.tags.all(), [furry_tag])
 
+    def test_prefetch_related_different_content_types(self):
+        TaggedItem.objects.create(content_object=self.platypus, tag='prefetch_tag_1')
+        TaggedItem.objects.create(
+            content_object=Vegetable.objects.create(name='Broccoli'),
+            tag='prefetch_tag_2',
+        )
+        TaggedItem.objects.create(
+            content_object=Animal.objects.create(common_name='Bear'),
+            tag='prefetch_tag_3',
+        )
+        qs = TaggedItem.objects.filter(
+            tag__startswith='prefetch_tag_',
+        ).prefetch_related('content_object', 'content_object__tags')
+        with self.assertNumQueries(4):
+            tags = list(qs)
+        for tag in tags:
+            self.assertSequenceEqual(tag.content_object.tags.all(), [tag])
+
 
 class ProxyRelatedModelTest(TestCase):
     def test_default_behavior(self):