diff --git a/.ci/.jenkins_exclude.yml b/.ci/.jenkins_exclude.yml
index adea37702..0206beaa6 100644
--- a/.ci/.jenkins_exclude.yml
+++ b/.ci/.jenkins_exclude.yml
@@ -1,6 +1,14 @@
 exclude:
   - PYTHON_VERSION: pypy-3  # pypy3 currently fails on CI, e.g. https://apm-ci.elastic.co/blue/organizations/jenkins/apm-agent-python%2Fapm-agent-python-mbp/detail/PR-1099/2/pipeline#step-3497-log-1
     FRAMEWORK: none
+  # Django
+    # Django 4.0 requires Python 3.8+
+  - PYTHON_VERSION: pypy-3 # current pypy-3 is compatible with Python 3.7
+    FRAMEWORK: django-4.0
+  - PYTHON_VERSION: python-3.6
+    FRAMEWORK: django-4.0
+  - PYTHON_VERSION: python-3.7
+    FRAMEWORK: django-4.0
   # Flask
   - PYTHON_VERSION: pypy-3
     FRAMEWORK: flask-0.11 # see https://github.com/pallets/flask/commit/6e46d0cd, 0.11.2 was never released
diff --git a/.ci/.jenkins_framework.yml b/.ci/.jenkins_framework.yml
index fe9f7d916..413541f63 100644
--- a/.ci/.jenkins_framework.yml
+++ b/.ci/.jenkins_framework.yml
@@ -6,6 +6,7 @@ FRAMEWORK:
   - django-2.0
   - django-3.1
   - django-3.2
+  - django-4.0
   - flask-0.12
   - flask-1.1
   - flask-2.0
diff --git a/.ci/.jenkins_framework_full.yml b/.ci/.jenkins_framework_full.yml
index 1906f27cb..8a417d079 100644
--- a/.ci/.jenkins_framework_full.yml
+++ b/.ci/.jenkins_framework_full.yml
@@ -9,6 +9,7 @@ FRAMEWORK:
   - django-3.0
   - django-3.1
   - django-3.2
+  - django-4.0
     #  - django-master
   - flask-0.10
   - flask-0.11
diff --git a/tests/contrib/django/testapp/urls.py b/tests/contrib/django/testapp/urls.py
index 7c49cfa68..c8bda4699 100644
--- a/tests/contrib/django/testapp/urls.py
+++ b/tests/contrib/django/testapp/urls.py
@@ -37,7 +37,7 @@
 from tests.contrib.django.testapp import views
 
 try:
-    from django.conf.urls import re_path
+    from django.urls import re_path
 except ImportError:
     # Django < 2
     from django.conf.urls import url as re_path
diff --git a/tests/requirements/reqs-django-4.0.txt b/tests/requirements/reqs-django-4.0.txt
new file mode 100644
index 000000000..a941313eb
--- /dev/null
+++ b/tests/requirements/reqs-django-4.0.txt
@@ -0,0 +1,2 @@
+Django>=4.0a1,<4.1
+-r reqs-base.txt