diff --git a/tests/cancun/eip1153_tstore/test_transient_storage_create_contexts.py b/tests/cancun/eip1153_tstore/test_transient_storage_create_contexts.py
new file mode 100644
index 0000000000..ad1ee23f33
--- /dev/null
+++ b/tests/cancun/eip1153_tstore/test_transient_storage_create_contexts.py
@@ -0,0 +1,185 @@
+"""
+abstract: Tests for [EIP-1153: Transient Storage](https://eips.ethereum.org/EIPS/eip-1153)
+
+    Test cases for `TSTORE` and `TLOAD` opcode calls in contract initcode.
+"""  # noqa: E501
+
+from enum import Enum, unique
+from textwrap import dedent
+
+import pytest
+
+from ethereum_test_tools import Account, Environment
+from ethereum_test_tools import Opcodes as Op
+from ethereum_test_tools import (
+    StateTestFiller,
+    TestAddress,
+    Transaction,
+    compute_create2_address,
+    compute_create_address,
+)
+
+from .spec import ref_spec_1153
+
+REFERENCE_SPEC_GIT_PATH = ref_spec_1153.git_path
+REFERENCE_SPEC_VERSION = ref_spec_1153.version
+
+pytestmark = [pytest.mark.valid_from("Shanghai")]
+
+# the address that creates the contract with create/create2
+creator_address = 0x100
+
+test_case_description = dedent(
+    """
+
+    - TSTORE/TLOAD in initcode should not be able to access the creator's transient storage.
+    - TSTORE/TLOAD in initcode should be able to access the created contract's transient storage.
+    - TSTORE/TLOAD in creator contract should be able to use its own transient storage.
+    """
+)
+
+
+@unique
+class TStorageInitCodeTestCases(Enum):
+    """
+    Test cases for transient storage opcodes use in contract initcode.
+    """
+
+    CREATE = {
+        "pytest_param": pytest.param(Op.CREATE, id="create"),
+        "description": (
+            "TSTORE0100: Test TLOAD and TSTORE behavior in a contract's initcode created by "
+            "CREATE:" + test_case_description
+        ),
+    }
+    CREATE2 = {
+        "pytest_param": pytest.param(Op.CREATE2, id="create2"),
+        "description": (
+            "TSTORE0101: Test TLOAD and TSTORE behavior in a contract's initcode created by "
+            "CREATE2:" + test_case_description
+        ),
+    }
+
+    def __init__(self, test_case):
+        self._value_ = test_case["pytest_param"]
+        self.description = test_case["description"]
+
+
+@pytest.fixture()
+def created_contract_initcode() -> bytes:
+    """
+    The following code will be executed in the constructor of the created contract.
+    """
+    bytecode = b""
+    # creator's transient storage shouldn't be available in the created contract's constructor
+    bytecode += Op.SSTORE(0, Op.TLOAD(0))
+    # constructor should be able to use its own transient storage / should not be able to use
+    # creator's transient storage
+    bytecode += Op.TSTORE(0, 1)
+    bytecode += Op.SSTORE(1, Op.TLOAD(0))
+    # dummy check
+    bytecode += Op.SSTORE(2, 2)
+    bytecode += Op.RETURN(0, 1)
+    return bytecode
+
+
+@pytest.fixture()
+def create2_salt() -> int:  # noqa: D103
+    return 0xDEADBEEF
+
+
+@pytest.fixture()
+def creator_contract_code(opcode: Op, create2_salt: int) -> bytes:  # noqa: D103
+    if opcode == Op.CREATE:
+        create_call = Op.CREATE(0, 0, Op.CALLDATASIZE)
+    elif opcode == Op.CREATE2:
+        create_call = Op.CREATE2(0, 0, Op.CALLDATASIZE, create2_salt)
+    else:
+        raise Exception("Invalid opcode specified for test.")
+    return (
+        Op.TSTORE(0, 420)
+        + Op.TSTORE(1, 69)
+        + Op.CALLDATACOPY(0, 0, Op.CALLDATASIZE)
+        + create_call
+        + Op.SSTORE(0, Op.TLOAD(0))
+        + Op.SSTORE(1, Op.TLOAD(1))
+    )
+
+
+@pytest.fixture()
+def created_contract_address(  # noqa: D103
+    opcode: Op, create2_salt: int, created_contract_initcode: bytes
+) -> str:
+    if opcode == Op.CREATE:
+        return compute_create_address(
+            address=creator_address,
+            nonce=1,
+        )
+    if opcode == Op.CREATE2:
+        return compute_create2_address(
+            address=creator_address,
+            salt=create2_salt,
+            initcode=created_contract_initcode,
+        )
+    raise Exception("invalid opcode for generator")
+
+
+@pytest.mark.parametrize(
+    "opcode",
+    [test_case._value_ for test_case in TStorageInitCodeTestCases],
+    ids=[test_case._value_.id for test_case in TStorageInitCodeTestCases],
+)
+def test_tload_tstore_in_initcode(
+    state_test: StateTestFiller,
+    creator_contract_code: str,
+    created_contract_initcode: str,
+    created_contract_address: str,
+) -> None:
+    """
+    Test transient storage in contract creation contexts:
+
+    - TSTORE/TLOAD in initcode should not be able to access the creator's transient storage.
+    - TSTORE/TLOAD in initcode should be able to access the created contract's transient storage.
+    - TSTORE/TLOAD in creator contract should be able to use its own transient storage.
+    """
+    pre = {
+        TestAddress: Account(balance=100_000_000_000_000),
+        creator_address: Account(
+            code=creator_contract_code,
+            nonce=1,
+        ),
+    }
+
+    tx = Transaction(
+        nonce=0,
+        to=creator_address,
+        data=created_contract_initcode,
+        gas_limit=1_000_000_000_000,
+        gas_price=10,
+    )
+
+    post = {
+        creator_address: Account(
+            nonce=2,
+            storage={
+                0: 420,
+                1: 69,
+            },
+        ),
+        created_contract_address: Account(
+            nonce=1,
+            code=Op.STOP,
+            storage={
+                0: 0,
+                1: 1,
+                2: 2,
+            },
+        ),
+    }
+
+    state_test(
+        env=Environment(),
+        pre=pre,
+        post=post,
+        txs=[tx],
+    )