diff --git a/newsfragments/993.misc.rst b/newsfragments/993.misc.rst
new file mode 100644
index 0000000000..77923d90c1
--- /dev/null
+++ b/newsfragments/993.misc.rst
@@ -0,0 +1 @@
+Relax some input types from ``Tuple[thing, ...]`` to ``Sequence[thing]``
diff --git a/p2p/handshake.py b/p2p/handshake.py
index 42f627ad06..41043beed2 100644
--- a/p2p/handshake.py
+++ b/p2p/handshake.py
@@ -9,6 +9,7 @@
     Dict,
     Iterable,
     NamedTuple,
+    Sequence,
     Type,
     Tuple,
 )
@@ -205,7 +206,7 @@ async def _do_p2p_handshake(transport: TransportAPI,
 
 async def negotiate_protocol_handshakes(transport: TransportAPI,
                                         p2p_handshake_params: DevP2PHandshakeParams,
-                                        protocol_handshakers: Tuple[Handshaker, ...],
+                                        protocol_handshakers: Sequence[Handshaker],
                                         token: CancelToken,
                                         ) -> Tuple[MultiplexerAPI, DevP2PReceipt, Tuple[HandshakeReceipt, ...]]:  # noqa: E501
     """