##FastQC 0.11.8 >>Basic Statistics pass #Measure Value Filename sample1_S1_L001_R2_001.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 82330 Sequences flagged as poor quality 0 Sequence length 301 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.27616907567108 34.0 34.0 34.0 34.0 34.0 2 33.14830559941698 34.0 34.0 34.0 34.0 34.0 3 11.308684562128022 2.0 2.0 34.0 2.0 34.0 4 26.101384671444187 23.0 23.0 34.0 23.0 34.0 5 31.597643629296734 32.0 32.0 34.0 31.0 34.0 6 2.0 2.0 2.0 2.0 2.0 2.0 7 26.07786954937447 28.0 25.0 28.0 24.0 28.0 8 31.003097291388315 34.0 28.0 34.0 24.0 34.0 9 35.147297461435684 37.0 34.0 37.0 34.0 37.0 10-14 35.650001214624076 37.8 36.2 38.0 31.8 38.0 15-19 35.65030001214624 38.0 36.6 38.0 31.4 38.0 20-24 35.53218510870885 38.0 36.2 38.0 30.6 38.0 25-29 35.33384671444188 38.0 36.0 38.0 29.4 38.0 30-34 35.01040689906473 37.4 34.8 38.0 28.6 38.0 35-39 34.890368031094376 37.2 34.6 38.0 27.8 38.0 40-44 35.28235637070326 38.0 36.0 38.0 28.4 38.0 45-49 35.1640544151585 37.8 35.8 38.0 27.8 38.0 50-54 32.759387829466775 37.0 32.4 38.0 17.0 38.0 55-59 32.52641321510992 36.4 31.4 37.8 18.6 38.0 60-64 34.85294060488279 37.2 35.6 38.0 27.8 38.0 65-69 33.80132151099235 37.0 34.0 38.0 23.6 38.0 70-74 33.649561520709334 37.0 33.0 38.0 22.6 38.0 75-79 32.17637799101178 35.8 30.0 37.8 18.6 38.0 80-84 30.61113081501275 34.8 26.0 37.2 11.0 38.0 85-89 31.60603425239888 36.0 28.6 37.6 13.0 38.0 90-94 31.608660269646542 36.6 27.8 38.0 11.0 38.0 95-99 26.95361593586785 30.4 18.2 35.4 10.8 37.4 100-104 18.96061217053322 17.8 11.0 24.4 10.2 32.8 105-109 20.01545487671566 17.6 11.0 26.8 10.4 33.6 110-114 19.95271954330135 19.0 11.0 26.4 10.6 33.2 115-119 19.510010931616662 18.6 11.0 25.0 10.2 32.6 120-124 18.144501396817684 15.6 11.0 24.0 10.0 31.6 125-129 17.960170047370337 14.0 11.0 24.6 10.0 32.0 130-134 17.761681039718205 15.2 11.0 24.4 9.8 30.8 135-139 17.941122312644232 17.0 11.0 24.2 8.4 30.6 140-144 17.196793392445038 13.8 11.0 22.4 10.0 30.0 145-149 17.396122919956273 13.8 11.0 23.8 10.0 30.2 150-154 15.865397789384184 11.0 10.8 21.0 9.8 26.2 155-159 16.850074092068503 12.4 11.0 22.6 9.6 28.0 160-164 16.779710919470425 13.6 11.0 22.4 9.2 28.4 165-169 16.226908781732053 11.8 11.0 22.2 9.0 26.8 170-174 16.758088181707762 13.4 11.0 22.6 9.0 27.8 175-179 16.828312887161424 13.4 11.0 22.4 9.0 27.6 180-184 16.53652860439694 12.0 11.0 22.8 9.0 27.4 185-189 15.209884610712985 11.0 10.4 20.8 9.0 25.2 190-194 15.67011296003887 13.4 10.0 22.2 7.6 26.6 195-199 15.202985545973522 11.0 10.0 20.2 9.0 25.8 200-204 15.210132394024049 11.4 10.0 20.0 9.0 25.6 205-209 14.856941576582049 11.0 9.6 19.8 9.0 24.8 210-214 14.89655775537471 11.8 9.6 19.0 9.0 24.6 215-219 15.439336815255677 11.2 10.4 19.8 9.0 26.4 220-224 12.63300376533463 9.8 8.8 16.2 8.0 20.8 225-229 14.13869063524839 11.6 10.0 17.2 8.8 23.4 230-234 13.629053807846471 11.2 9.2 17.0 8.8 22.6 235-239 12.257789384185594 10.2 8.8 15.6 7.8 18.6 240-244 12.865332199684198 10.6 9.2 16.4 8.4 19.0 245-249 13.37639013725252 11.0 9.2 17.0 9.0 19.0 250-254 13.399407263451963 10.8 9.4 17.0 9.0 19.6 255-259 12.844222033280698 10.8 9.4 16.6 8.0 18.0 260-264 13.045677152921172 10.8 9.6 16.8 8.4 18.0 265-269 12.269036803109438 10.6 8.6 15.8 7.8 17.8 270-274 11.610943762905382 9.8 8.4 14.8 6.0 17.4 275-279 11.012083080286653 9.4 7.8 13.4 7.0 16.8 280-284 10.96915097777238 9.8 8.2 14.0 6.0 17.2 285-289 11.321559577310822 9.8 8.4 14.8 7.2 17.4 290-294 11.89309850601239 10.6 9.0 15.2 7.6 17.8 295-299 11.473321996841978 10.4 8.8 15.0 3.2 17.6 300-301 10.231841370095957 9.5 7.5 14.0 4.5 17.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5962914245882303 1101 2 0.23153468164522195 1101 3 -9.792674112344926 1101 4 -3.2437637841871094 1101 5 -0.814573297477093 1101 6 0.0 1101 7 0.20215731330333853 1101 8 -0.43102593273251344 1101 9 -0.10273295387844428 1101 10-14 0.01623705968011535 1101 15-19 -0.01330553810645796 1101 20-24 0.18923631635838234 1101 25-29 0.14076736102921927 1101 30-34 0.24084493336675905 1101 35-39 0.32348429362815523 1101 40-44 0.1255637753829646 1101 45-49 0.13309971590945224 1101 50-54 -0.23329631139360174 1101 55-59 -0.09232307270402629 1101 60-64 -0.06557445912336846 1101 65-69 -0.05167547316963805 1101 70-74 0.5317344364909218 1101 75-79 0.2654295501157442 1101 80-84 -0.7090596500242796 1101 85-89 0.020353487099104228 1101 90-94 -0.023599414015542663 1101 95-99 0.8721924336233648 1101 100-104 1.0170417520191997 1101 105-109 0.03377078717690196 1101 110-114 0.09814260473661207 1101 115-119 -0.48154298132917717 1101 120-124 -0.12736844160048122 1101 125-129 0.24215706020212124 1101 130-134 0.39203280041439115 1101 135-139 0.4506569887149219 1101 140-144 -0.5177284441640353 1101 145-149 -0.526967519083609 1101 150-154 -0.0014589642895774801 1101 155-159 0.7380617245227903 1101 160-164 0.6486200142500813 1101 165-169 -0.2615994808671349 1101 170-174 0.403951079546097 1101 175-179 0.6660402340372613 1101 180-184 1.094013883875487 1101 185-189 0.3132081055579299 1101 190-194 0.7887278978298564 1101 195-199 -0.3251371152822191 1101 200-204 -0.22957718678734196 1101 205-209 -3.149701594068647 1101 210-214 0.08305133781695595 1101 215-219 0.5271366927327463 1101 220-224 0.7262860801501656 1101 225-229 0.30131304101262835 1101 230-234 -1.2384477920780217 1101 235-239 1.2683792083381 1101 240-244 0.2656487228766924 1101 245-249 0.146231562225136 1101 250-254 0.7865861428112719 1101 255-259 -0.138618708259715 1101 260-264 1.0523604219624847 1101 265-269 -0.6146974557623999 1101 270-274 -9.599365676132617 1101 275-279 -2.72605734866732 1101 280-284 -0.7012397443635869 1101 285-289 1.2880139646027509 1101 290-294 -0.08294386698454126 1101 295-299 1.6712746017080722 1101 300-301 3.521796881489937 1102 1 0.5359417241699447 1102 2 0.1919296019451764 1102 3 -9.792674112344926 1102 4 -3.3603557124382313 1102 5 -0.4918163920243295 1102 6 0.0 1102 7 -0.29855866815650955 1102 8 -0.16837378667229785 1102 9 0.15093174069893678 1102 10-14 0.7036207126928886 1102 15-19 0.37653883013410905 1102 20-24 0.5455463740137318 1102 25-29 0.8426703643830322 1102 30-34 0.8476015373536399 1102 35-39 0.34468111316314065 1102 40-44 0.9509622755902214 1102 45-49 0.47454373746425915 1102 50-54 -0.2731689420602095 1102 55-59 0.621800603672348 1102 60-64 0.9048894214961436 1102 65-69 0.8325390198024465 1102 70-74 1.3509868011139048 1102 75-79 1.0720768727219117 1102 80-84 0.8212150607568915 1102 85-89 1.0840683003786715 1102 90-94 1.0236252157466907 1102 95-99 -0.06693783731909164 1102 100-104 0.09699427114344772 1102 105-109 1.3159579221205675 1102 110-114 0.8766820025585211 1102 115-119 0.23186848191083342 1102 120-124 -0.45636192240087325 1102 125-129 -0.005003508061054873 1102 130-134 0.8620067989824278 1102 135-139 1.1985410750848935 1102 140-144 0.28410296104378574 1102 145-149 -0.04090654760981849 1102 150-154 0.09706008458558024 1102 155-159 0.3478141456706254 1102 160-164 0.5315419722709578 1102 165-169 0.8563528121576951 1102 170-174 0.17886535018708827 1102 175-179 -0.2880465127690819 1102 180-184 0.05268291202486708 1102 185-189 -0.7554620530884879 1102 190-194 1.555954788586158 1102 195-199 -0.64289345548646 1102 200-204 -0.017156792620706796 1102 205-209 0.41062926473227 1102 210-214 0.2115134850076732 1102 215-219 -0.2802944411557906 1102 220-224 -0.3582261103762683 1102 225-229 0.10208554732457209 1102 230-234 1.2958740981130301 1102 235-239 1.1647352395398194 1102 240-244 0.6320729607332698 1102 245-249 0.024876473104288976 1102 250-254 -0.44149917203215594 1102 255-259 0.8185542954709266 1102 260-264 1.1977687198107727 1102 265-269 1.3881212501953168 1102 270-274 1.6876298395624474 1102 275-279 0.6289337957725181 1102 280-284 1.4492369485557326 1102 285-289 0.04090992119166437 1102 290-294 0.995682202277246 1102 295-299 1.6694695747645607 1102 300-301 3.4215406354937805 1103 1 0.34024355089329816 1103 2 0.3242263037486097 1103 3 -9.792674112344926 1103 4 -3.3476598880832142 1103 5 -0.703801716117276 1103 6 0.0 1103 7 0.5682128264176747 1103 8 0.062480560773977345 1103 9 0.6040406646598839 1103 10-14 0.09739315848326413 1103 15-19 0.257155373530928 1103 20-24 0.05936618648824776 1103 25-29 0.7250301570567288 1103 30-34 0.33987727357555997 1103 35-39 0.43000326485300633 1103 40-44 0.025894816890478012 1103 45-49 0.4155799807426561 1103 50-54 -0.3477093493360428 1103 55-59 -0.12835566756015027 1103 60-64 0.5065161953202306 1103 65-69 0.7607513157277452 1103 70-74 0.7036977672777951 1103 75-79 0.8801990942970441 1103 80-84 0.767003502509457 1103 85-89 0.6509620941783005 1103 90-94 1.1094792720043216 1103 95-99 0.648128262500375 1103 100-104 0.42806798069249297 1103 105-109 1.0422250779841349 1103 110-114 0.6267140333080441 1103 115-119 0.1240083301044912 1103 120-124 -0.18698137768400613 1103 125-129 0.287000452104337 1103 130-134 0.9190254156422988 1103 135-139 0.6150572942916988 1103 140-144 0.3544386044736001 1103 145-149 0.022510453923775486 1103 150-154 -0.1542473523720833 1103 155-159 0.5241579812072814 1103 160-164 0.06346595262543175 1103 165-169 0.6115097628303445 1103 170-174 0.009273208397637234 1103 175-179 1.2286580084197425 1103 180-184 0.4570390939595228 1103 185-189 0.8515070615039448 1103 190-194 1.4165608491922175 1103 195-199 -0.5963110393973192 1103 200-204 -0.27673786924214383 1103 205-209 0.5884445734892871 1103 210-214 0.11350715650807075 1103 215-219 0.1981323637284138 1103 220-224 -0.3814551981793457 1103 225-229 0.6430752004218476 1103 230-234 1.5542438300253636 1103 235-239 -0.00567223041922027 1103 240-244 -0.8473128253789728 1103 245-249 -0.12685221674099445 1103 250-254 0.39511682781254187 1103 255-259 -0.4603655888301237 1103 260-264 0.6253423420032274 1103 265-269 1.1610601199951738 1103 270-274 -0.23746091422785476 1103 275-279 -0.7540685531491125 1103 280-284 -1.590620951391852 1103 285-289 0.01340230175579471 1103 290-294 0.5308325526742319 1103 295-299 1.1438236213159154 1103 300-301 -0.1946533349602788 1104 1 0.4870292175027515 1104 2 -0.22754057841475372 1104 3 -9.792674112344926 1104 4 -3.3494906564338045 1104 5 -0.7009983520804326 1104 6 0.0 1104 7 0.28783828172594994 1104 8 0.7640653196274592 1104 9 0.3131964134632099 1104 10-14 0.5242406135245616 1104 15-19 0.42901112424129906 1104 20-24 0.6069833270569305 1104 25-29 0.6806111399504786 1104 30-34 0.6033629802330793 1104 35-39 0.7466842343974136 1104 40-44 0.5635380840491706 1104 45-49 0.5004894912028277 1104 50-54 0.9510186480703595 1104 55-59 1.1863686683095551 1104 60-64 0.8823691235960709 1104 65-69 0.6955721483459385 1104 70-74 1.2273661693602804 1104 75-79 1.2788527167392196 1104 80-84 1.5183012502829776 1104 85-89 1.509954790175403 1104 90-94 1.61048047344606 1104 95-99 0.7867631958489625 1104 100-104 0.805095651993291 1104 105-109 1.2471643384300002 1104 110-114 1.7208612957372402 1104 115-119 0.7835960258155339 1104 120-124 0.4450189274440497 1104 125-129 0.2343200928160485 1104 130-134 0.7870537162697175 1104 135-139 0.8148742174566372 1104 140-144 0.3771592025245951 1104 145-149 0.06801267563745128 1104 150-154 0.454654758732671 1104 155-159 -0.08757037853869676 1104 160-164 -0.04582710380419286 1104 165-169 0.6546815689977414 1104 170-174 0.43310370976117696 1104 175-179 -0.17183782467520814 1104 180-184 -0.26580546236520775 1104 185-189 0.3101984435433458 1104 190-194 0.5753380484980521 1104 195-199 0.883010813492815 1104 200-204 -0.7725824064963724 1104 205-209 0.46013441081788997 1104 210-214 0.45459837072703735 1104 215-219 -0.7232094369085402 1104 220-224 -0.6083713070337815 1104 225-229 0.6813115602441115 1104 230-234 0.504427860385606 1104 235-239 1.2978539057060345 1104 240-244 0.2256013382840898 1104 245-249 -0.12104715542932709 1104 250-254 0.7141358411046852 1104 255-259 0.5409540900225949 1104 260-264 -0.547672895329189 1104 265-269 -0.6397446552589408 1104 270-274 1.4834537071272944 1104 275-279 -0.18973039497835487 1104 280-284 1.9716881051903332 1104 285-289 -1.1832184049970706 1104 290-294 -1.9188117824326572 1104 295-299 2.098931655719106 1104 300-301 3.8459000910557037 1105 1 0.10067421218602846 1105 2 0.17578996996744678 1105 3 -9.792674112344926 1105 4 -3.3860051634974546 1105 5 -0.6775477976219797 1105 6 0.0 1105 7 -0.23173865887863698 1105 8 0.3768558406665008 1105 9 0.2603139890366535 1105 10-14 0.5729380159246773 1105 15-19 0.48192772761361624 1105 20-24 0.3657141488707012 1105 25-29 0.21649903300836115 1105 30-34 0.2930979035203407 1105 35-39 0.6790478983804036 1105 40-44 0.35774285391092775 1105 45-49 0.3633445724727622 1105 50-54 0.3710285219880305 1105 55-59 0.5894865476958557 1105 60-64 0.5656556191148283 1105 65-69 0.7829449267144568 1105 70-74 1.4682745697856276 1105 75-79 1.5410887793246637 1105 80-84 0.7179252818795163 1105 85-89 1.736747285997975 1105 90-94 1.49748867639304 1105 95-99 0.9116302777265268 1105 100-104 0.4282993585100776 1105 105-109 0.9188185994052418 1105 110-114 1.7769603387267594 1105 115-119 0.49844108126137954 1105 120-124 0.08665647871246662 1105 125-129 -0.21218226173354893 1105 130-134 0.6411779757258955 1105 135-139 0.7452744908882032 1105 140-144 -0.45219671423068064 1105 145-149 -0.21405097315489385 1105 150-154 -0.16978698515309532 1105 155-159 0.28456998299859393 1105 160-164 0.04170897397190032 1105 165-169 -0.2514212716524149 1105 170-174 0.5749957042925473 1105 175-179 0.4361068813858573 1105 180-184 0.11122628607775198 1105 185-189 0.22163319973352813 1105 190-194 0.9421616851378811 1105 195-199 -0.10817101849867683 1105 200-204 -0.517732793857757 1105 205-209 -0.08253990180553394 1105 210-214 -0.28738476765971477 1105 215-219 -0.13641010529681097 1105 220-224 -0.766897055172926 1105 225-229 -0.20528649535364174 1105 230-234 0.31836907259888214 1105 235-239 0.47439195826565594 1105 240-244 0.20704976605258274 1105 245-249 -0.2218066875575353 1105 250-254 0.29509816831112623 1105 255-259 0.7871564293815485 1105 260-264 0.19826740544553445 1105 265-269 0.13776559842478875 1105 270-274 0.8506343238673821 1105 275-279 1.1171547637638604 1105 280-284 1.4259262944657394 1105 285-289 0.7888687892912465 1105 290-294 0.12573477688680157 1105 295-299 1.0267874965211696 1105 300-301 -0.36674991653961797 1106 1 0.44319268509243415 1106 2 0.0477111522334539 1106 3 -9.792674112344926 1106 4 -3.319656641329967 1106 5 -0.8628926252081861 1106 6 0.0 1106 7 0.3633968091016584 1106 8 -0.17656164701822874 1106 9 -0.11376236564314723 1106 10-14 -0.15477134368124013 1106 15-19 -0.16619419356864285 1106 20-24 0.0874506020726713 1106 25-29 -0.05686919359263243 1106 30-34 0.1495634207617158 1106 35-39 0.26817967177941426 1106 40-44 -0.05321773722207723 1106 45-49 0.3594652621279337 1106 50-54 0.2191196549929515 1106 55-59 0.12443112897663866 1106 60-64 0.24471965852369237 1106 65-69 0.7837762075026333 1106 70-74 0.7256945205245415 1106 75-79 0.5289274492754039 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-0.053286065501765734 2118 130-134 -3.4415806449637607 2118 135-139 -0.38176959016405476 2118 140-144 1.2037044588940944 2118 145-149 0.7392284438376429 2118 150-154 0.5807640280001198 2118 155-159 0.9005671390597385 2118 160-164 0.643681956552772 2118 165-169 0.646561309222097 2118 170-174 0.8342118436455515 2118 175-179 0.7516418973830135 2118 180-184 0.4305295210581974 2118 185-189 0.6516989658195591 2118 190-194 1.3487382937407961 2118 195-199 -2.010712788850613 2118 200-204 -0.9174572872811471 2118 205-209 0.5623143463281313 2118 210-214 0.8962714712311506 2118 215-219 0.8172692405972537 2118 220-224 0.825930886526324 2118 225-229 1.3664607495805932 2118 230-234 1.420990828731128 2118 235-239 -5.267893857589302 2118 240-244 -2.1991091390309574 2118 245-249 0.482885811553734 2118 250-254 0.7629527719371474 2118 255-259 -1.1909274668146814 2118 260-264 -5.363259434227413 2118 265-269 -2.601000843096717 2118 270-274 0.332593086753981 2118 275-279 2.4687100428253608 2118 280-284 3.085453489631041 2118 285-289 -3.277341662184046 2118 290-294 -3.4580369970200593 2118 295-299 1.687318957594755 2118 300-301 -2.9200293335321534 2119 1 -0.046294642096505356 2119 2 -0.2527880797173907 2119 3 21.11055169410669 2119 4 7.041182452372027 2119 5 1.3010858694154699 2119 6 0.0 2119 7 -0.1011769236172384 2119 8 -0.4379383751288266 2119 9 -0.3824835637998305 2119 10-14 -0.6503934634969042 2119 15-19 -0.35049718896034676 2119 20-24 -0.9625301966984594 2119 25-29 -0.7187067511963079 2119 30-34 -1.1430441061200085 2119 35-39 -1.4991686385124368 2119 40-44 -0.8294466158703031 2119 45-49 -0.7503465658290551 2119 50-54 -0.7091253526875327 2119 55-59 -1.2319724808543882 2119 60-64 -0.9365783445485079 2119 65-69 -1.1161373869674094 2119 70-74 -0.7187851261728468 2119 75-79 -1.4718886029519425 2119 80-84 -1.196563038476441 2119 85-89 -1.5275185649475205 2119 90-94 -0.36266149045902196 2119 95-99 -1.3677913018062924 2119 100-104 -0.4773921505740937 2119 105-109 -0.6665725759748113 2119 110-114 -0.7903366579361979 2119 115-119 -0.6463030789165991 2119 120-124 0.2292593708148054 2119 125-129 -0.019088083016452373 2119 130-134 -2.2555591395874153 2119 135-139 -0.5767036329531017 2119 140-144 0.48816294464955234 2119 145-149 -0.2769449294025783 2119 150-154 0.29647182092773683 2119 155-159 -3.172085560197548 2119 160-164 0.19434152866229581 2119 165-169 0.4201119133679825 2119 170-174 -0.03988193753170677 2119 175-179 0.03099008494536548 2119 180-184 0.7542741170009961 2119 185-189 1.2756098404454335 2119 190-194 1.1236967240700313 2119 195-199 0.44787273381871096 2119 200-204 0.7662030607955579 2119 205-209 -1.2455450927584426 2119 210-214 0.7743226850655347 2119 215-219 0.9294408401981809 2119 220-224 0.4842060223205831 2119 225-229 -0.737053273255011 2119 230-234 0.3033431178343289 2119 235-239 0.784273307913411 2119 240-244 -0.09325612938679484 2119 245-249 0.6652159301658624 2119 250-254 0.8391749202577348 2119 255-259 0.05506521692326238 2119 260-264 -0.6098063802422686 2119 265-269 -0.5540215725058744 2119 270-274 0.8221397002114692 2119 275-279 0.8751173147464346 2119 280-284 1.6924261090745638 2119 285-289 -2.775146783567484 2119 290-294 -1.6636793869989965 2119 295-299 -0.7741487289144988 2119 300-301 1.2794773730413986 >>END_MODULE >>Per sequence quality scores warn #Quality Count 12 5.0 13 20.0 14 94.0 15 283.0 16 834.0 17 2147.0 18 5038.0 19 10649.0 20 19862.0 21 27719.0 22 11579.0 23 2076.0 24 962.0 25 604.0 26 308.0 27 126.0 28 20.0 29 3.0 30 0.0 31 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 99.42649798482837 0.12906839407259488 0.13272127315012117 0.3117123479489084 2 0.38017733511478197 97.35333414308272 0.1554718814526904 2.1110166403498116 3 0.2928972418843056 1.0048002603531039 1.0902286225693596 97.61207387519323 4 1.8450139681768494 0.5550832017490587 96.93428883760477 0.6656139924693307 5 0.2769342888376048 96.17514879144905 0.0971699259079315 3.4507469938054176 6 NaN NaN NaN NaN 7 0.16640349811733268 8.048099113324426 19.921049435199805 71.86444795335844 8 32.64180736062189 8.40034009474068 0.3643872221547431 58.59346532248269 9 76.99502004129721 0.1445402647880481 17.78574031337301 5.074699380541722 10-14 43.10190695979594 24.960281792785132 21.524110287865906 10.413700959553019 15-19 28.192153528482933 41.87149277298676 14.928701566865055 15.00765213166525 20-24 39.072755982023565 25.195918863111867 16.232479047734728 19.498846107129843 25-29 42.058301955544756 43.07615692943034 4.1523138588606825 10.713227256164219 30-34 38.85169440058302 2.5922506984088423 26.645694157658205 31.910360743349937 35-39 46.77687355763391 20.43993683954816 29.632454755253253 3.150734847564679 40-44 37.77098658063764 57.17707533694163 2.6870305409528625 2.36490754146787 45-49 45.91584128349209 18.936804437026726 30.87601153471468 4.2713427447665 50-54 5.007094679961903 18.56303574552452 48.02345131883297 28.406418255680606 55-59 24.38364883775169 4.680819015781699 42.338976133094924 28.59655601337169 60-64 34.94910725130572 26.8067533098506 33.94534191667679 4.29879752216689 65-69 15.825579982995263 13.588728288594679 34.91339730353455 35.6722944248755 70-74 64.15474310700839 1.4434592493623224 26.555326126563827 7.846471517065469 75-79 27.61909389044091 2.6933074213530914 18.19433985181586 51.49325883639013 80-84 13.126199441272925 25.71164824486821 37.4492894449168 23.71286286894206 85-89 4.188054328101504 60.027402118805675 33.19227396288568 2.5922695902071435 90-94 0.9498429479626962 90.00648613982368 5.441555507510076 3.6021154047035444 95-99 3.5032187537957005 83.73812704967813 3.3217539171626385 9.436900279363538 100-104 5.530669257864691 56.78173205392931 15.938540021863234 21.749058666342766 105-109 9.874893720393537 58.0233207822179 7.183772622373376 24.918012875015183 110-114 8.599802258720416 54.62226314165709 16.928256840172086 19.849677759450405 115-119 12.264059273654803 50.976800680189484 10.486821328798737 26.27231871735698 120-124 13.95554475889712 44.63136159358678 11.017612049070813 30.39548159844528 125-129 22.404469816591764 45.01761204907081 11.917648487793029 20.660269646544393 130-134 13.079358126941148 42.81302261686709 21.6517679382018 22.455851317989968 135-139 12.495888936505247 48.572090612822535 16.384955002541385 22.547065448130837 140-144 13.666750158675347 39.89912868388223 18.26675453592629 28.16736662151613 145-149 18.483747837870055 39.57543187861419 17.222110044203546 24.718710239312205 150-154 19.757236411103232 34.50854727068852 19.57321307203655 26.1610032461717 155-159 25.30930528308211 36.56333487780593 19.881059179589304 18.24630065952266 160-164 23.299550308741388 37.01101381533445 20.01887654589173 19.670559330032436 165-169 22.6222691897444 35.68077667601627 19.613192825820725 22.083761308418598 170-174 22.476432502588338 36.534203213006585 20.50434196773123 20.485022316673852 175-179 25.06110877730856 35.70398539776593 22.119534910001203 17.1153709149243 180-184 28.263129055798945 31.12494747886255 23.403664562544996 17.20825890279351 185-189 27.60664938805606 29.886982104061293 22.529351854820217 19.97701665306243 190-194 25.320256051271684 36.22265506023757 19.06563297440767 19.391455914083075 195-199 27.628574015573754 29.191734240125555 25.23667478218878 17.943016962111912 200-204 22.690397125838523 32.65204694991235 20.732337531000343 23.925218393248787 205-209 25.881670826085863 33.618442612619425 18.936701202450276 21.56318535884444 210-214 27.426821361628484 31.394348537940882 24.024389936250135 17.1544401641805 215-219 25.636667739138762 32.74003521965621 17.39492434159628 24.22837269960875 220-224 22.482150570419492 30.486317554691666 22.39830271616231 24.63322915872653 225-229 25.47503001170643 33.02207320619471 20.77481154139627 20.728085240702583 230-234 25.178350250644094 31.325552472487644 24.301413831077834 19.194683445790425 235-239 25.10247794415014 27.952887606284982 22.63501874809648 24.309615701468392 240-244 24.38486835588486 29.261594389523943 23.498325970243876 22.855211284347327 245-249 25.419771596512035 30.425204446731964 21.03345989263594 23.121564064120065 250-254 26.407608437009223 32.50618047745597 20.834032576814508 20.2521785087203 255-259 25.073294601977608 33.990483885447524 19.894322393649084 21.041899118925784 260-264 25.222184549312125 33.937212958111154 20.046440423717492 20.79416206885923 265-269 25.016669915162954 32.68585673105528 18.77673597931905 23.520737374462716 270-274 28.58673862760216 28.835517861732203 22.61809303520946 19.95965047545618 275-279 25.602172066496788 29.46446628645391 22.600159770254418 22.333201876794888 280-284 29.11762983781252 28.987752103859606 22.736587728683286 19.158030329644593 285-289 28.04148616467068 29.28453810431275 23.664505488406025 19.00947024261054 290-294 25.93577061077213 30.920719223206504 22.07942267539681 21.064087490624555 295-299 27.763429049920884 29.60861503662097 22.46331105806613 20.164644855392012 300-301 25.623237573306078 32.642498314594526 18.759106626361916 22.975157485737483 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.25 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 0.25 24 0.25 25 0.5 26 1.5 27 2.25 28 4.5 29 10.25 30 20.25 31 51.75 32 97.5 33 199.5 34 371.5 35 577.25 36 851.75 37 1133.5 38 1400.5 39 1643.75 40 1796.0 41 1965.25 42 2035.25 43 2000.5 44 1985.25 45 1913.25 46 1706.25 47 1559.0 48 1376.0 49 1165.0 50 944.5 51 748.75 52 577.5 53 416.75 54 291.5 55 200.25 56 137.0 57 84.5 58 51.5 59 33.0 60 22.25 61 10.75 62 7.5 63 4.75 64 4.75 65 3.75 66 2.75 67 2.75 68 2.25 69 2.75 70 1.5 71 2.0 72 1.25 73 1.0 74 2.0 75 1.25 76 1.0 77 1.25 78 1.25 79 0.75 80 1.0 81 0.25 82 0.25 83 0.25 84 0.0 85 0.75 86 0.0 87 0.25 88 0.25 89 0.25 90 0.0 91 0.0 92 0.25 93 0.25 94 0.0 95 0.0 96 0.25 97 0.25 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.24656868699137616 2 0.0 3 70.14211101664036 4 0.0 5 0.0 6 100.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0014575488886189724 45-49 0.005587270739706061 50-54 0.017976436292967326 55-59 0.008259443702174176 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 7.287744443094862E-4 90-94 7.287744443094862E-4 95-99 0.0 100-104 0.0 105-109 0.0 110-114 2.4292481476982875E-4 115-119 0.0 120-124 0.0 125-129 0.0 130-134 2.3869792299283374 135-139 2.500668043240617 140-144 0.10518644479533584 145-149 0.14502611441758775 150-154 0.9942912668529089 155-159 1.4337422567715292 160-164 2.7093404591279 165-169 1.6135066197012025 170-174 1.9229928337179643 175-179 1.114781974978744 180-184 1.13470180978987 185-189 1.4913154378719786 190-194 5.088546094983602 195-199 3.4150370460342527 200-204 1.7536742378233938 205-209 3.634155228956638 210-214 3.7063038989432773 215-219 1.090975343131301 220-224 4.392080651038503 225-229 2.260901251062796 230-234 4.485120855095348 235-239 2.690635248390623 240-244 1.9030729989068382 245-249 2.2997692214259686 250-254 3.0144540264788047 255-259 5.292117089760719 260-264 5.4254828130693555 265-269 5.277784525689299 270-274 5.477954573059638 275-279 3.9067168711283857 280-284 8.72391594801409 285-289 8.091096805538687 290-294 4.130207700716628 295-299 10.646908781732053 300-301 16.221911818292238 >>END_MODULE >>Sequence Length Distribution pass #Length Count 301 82330.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.744928944491676 #Duplication Level Percentage of deduplicated Percentage of total 1 78.92114827065213 23.4750394752824 2 8.007676916166442 4.763755617636342 3 3.0911837968067295 2.758411271711405 4 1.604802156070072 1.9093890440908539 5 1.090285434276614 1.621523138588607 6 0.8166932091959656 1.4575488886189725 7 0.5267671199313978 1.0968055386857767 8 0.5022663236555188 1.1951900886675575 9 0.3920127404140635 1.0494351998056601 >10 3.818040752991139 25.363779910117817 >50 0.8330270733798849 17.423782339365967 >100 0.3960962064600433 17.88533948742864 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNN 331 0.4020405684440666 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 318 0.3862504554840277 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 311 0.3777480869670837 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 308 0.37410421474553623 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 295 0.3583141017854974 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 246 0.2987975221668894 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 234 0.2842220332806996 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 224 0.27207579254220815 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 210 0.2550710555083202 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 207 0.2514271832867727 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 206 0.25021255921292357 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 204 0.2477833110652253 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 202 0.245354062917527 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 201 0.2441394388436779 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 196 0.23806631847443216 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 193 0.23442244625288475 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 192 0.2332078221790356 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 187 0.22713470180978987 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 182 0.22106158144054416 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 181 0.21984695736669502 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 179 0.21741770921899672 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 179 0.21741770921899672 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 179 0.21741770921899672 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 178 0.21620308514514758 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 171 0.20770071662820355 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 170 0.2064860925543544 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 168 0.20405684440665614 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 166 0.20162759625895785 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 163 0.19798372403741044 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 157 0.19069597959431556 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 156 0.1894813555204664 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 156 0.1894813555204664 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 154 0.18705210737276812 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 154 0.18705210737276812 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 154 0.18705210737276812 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 154 0.18705210737276812 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 153 0.185837483298919 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 151 0.1834082351512207 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 150 0.18219361107737156 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 149 0.1809789870035224 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 148 0.17976436292967327 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 146 0.17733511478197497 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 145 0.17612049070812583 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 145 0.17612049070812583 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 145 0.17612049070812583 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 142 0.17247661848657841 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 142 0.17247661848657841 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 141 0.17126199441272927 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 141 0.17126199441272927 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 140 0.1700473703388801 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 140 0.1700473703388801 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 134 0.16275962589578527 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 133 0.16154500182193612 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 133 0.16154500182193612 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 132 0.16033037774808698 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 131 0.1591157536742378 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 128 0.1554718814526904 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 128 0.1554718814526904 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 127 0.15425725737884124 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 124 0.15061338515729383 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 123 0.1493987610834447 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 123 0.1493987610834447 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 121 0.14696951293574637 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 120 0.14575488886189725 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 119 0.1445402647880481 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 119 0.1445402647880481 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 119 0.1445402647880481 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 117 0.1421110166403498 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 116 0.14089639256650066 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 116 0.14089639256650066 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 116 0.14089639256650066 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 116 0.14089639256650066 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 114 0.1384671444188024 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 113 0.13725252034495322 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 112 0.13603789627110408 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 112 0.13603789627110408 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 112 0.13603789627110408 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 112 0.13603789627110408 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 111 0.13482327219725496 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 111 0.13482327219725496 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 110 0.1336086481234058 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 109 0.13239402404955666 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 109 0.13239402404955666 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 109 0.13239402404955666 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 108 0.13117939997570752 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 108 0.13117939997570752 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 107 0.12996477590185837 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 105 0.1275355277541601 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 105 0.1275355277541601 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 104 0.12632090368031093 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 103 0.12510627960646178 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 102 0.12389165553261265 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 102 0.12389165553261265 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 102 0.12389165553261265 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 102 0.12389165553261265 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 102 0.12389165553261265 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 102 0.12389165553261265 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 100 0.12146240738491437 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 100 0.12146240738491437 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 99 0.12024778331106523 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 99 0.12024778331106523 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 98 0.11903315923721608 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 98 0.11903315923721608 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 97 0.11781853516336695 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 96 0.1166039110895178 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 96 0.1166039110895178 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 96 0.1166039110895178 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 95 0.11538928701566864 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 95 0.11538928701566864 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 94 0.1141746629418195 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 94 0.1141746629418195 No Hit NNNNNNNNN 94 0.1141746629418195 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 93 0.11296003886797036 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 93 0.11296003886797036 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 93 0.11296003886797036 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 92 0.11174541479412121 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 90 0.10931616664642294 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 90 0.10931616664642294 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 90 0.10931616664642294 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 89 0.10810154257257379 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 89 0.10810154257257379 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 89 0.10810154257257379 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 88 0.10688691849872466 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 88 0.10688691849872466 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 88 0.10688691849872466 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 87 0.10567229442487551 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 86 0.10445767035102635 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 86 0.10445767035102635 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 86 0.10445767035102635 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 86 0.10445767035102635 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 86 0.10445767035102635 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 86 0.10445767035102635 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 85 0.1032430462771772 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 85 0.1032430462771772 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 85 0.1032430462771772 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 85 0.1032430462771772 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 84 0.10202842220332807 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 84 0.10202842220332807 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 84 0.10202842220332807 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 84 0.10202842220332807 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 84 0.10202842220332807 Illumina Single End PCR Primer 1 (96% over 33bp) NNNNNNNNN 83 0.10081379812947892 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 83 0.10081379812947892 Illumina Single End PCR Primer 1 (100% over 33bp) NNNNNNNNN 83 0.10081379812947892 Illumina Single End PCR Primer 1 (100% over 33bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-14 0.25312765699016154 0.0 0.0 0.0 0.0 15-19 46.353455605490105 0.0 0.0 0.0 0.0 20-24 88.0991133244261 0.0 0.0 0.0 0.0 25-29 88.66269889469208 0.0 0.0 0.0 0.0 30-34 88.75525324911939 0.0 0.0 0.0 0.0 35-39 88.89882181464837 0.0 0.0 0.0 0.0 40-44 89.02781489129113 0.0 0.0 0.0 0.0 45-49 89.13130086238309 0.0 0.0 0.0 0.0 50-54 89.30159115753675 0.0 0.0 0.0 0.0 55-59 89.70678974857282 0.0 0.0 0.0 0.0 60-64 90.04372646665857 0.0 0.0 0.0 0.0 65-69 90.1367666707154 0.0 0.0 0.0 0.0 70-74 90.2414672658812 0.0 0.0 0.0 0.0 75-79 90.32260415401433 0.0 0.0 0.0 0.0 80-84 90.37264666585692 0.0 0.0 0.0 0.0 85-89 90.42949107251307 0.0 0.0 0.0 0.0 90-94 90.46398639621039 0.0 0.0 0.0 0.0 95-99 90.72998906838333 0.0 0.0 0.0 0.0 100-104 90.76788533948744 0.0 0.0 0.0 0.0 105-109 90.79630754281551 0.0 0.0 0.0 0.0 110-114 90.81841370095955 0.0 0.0 0.0 0.0 115-119 90.84537835539899 0.0 0.0 0.0 0.0 120-124 90.86991376169075 0.0 0.0 0.0 0.0 125-129 90.88303170168832 0.0 0.0 0.0 0.0 130-134 90.89129114539051 0.0 0.0 0.0 0.0 135-139 90.89882181464837 0.0 0.0 0.0 0.0 140-144 90.90368031094377 0.0 0.0 0.0 0.0 145-149 90.91145390501642 0.0 0.0 0.0 0.0 150-154 90.91364022834932 0.0 0.0 0.0 0.0 155-159 90.9146119276084 0.0 0.0 0.0 0.0 160-164 90.91582655168226 0.0 0.0 0.0 0.0 165-169 90.91582655168226 0.0 0.0 0.0 0.0 170-174 90.91655532612656 0.0 0.0 0.0 0.0 175-179 90.9170411757561 0.0 0.0 0.0 0.0 180-184 90.9170411757561 0.0 0.0 4.858496295396575E-4 0.0 185-189 90.9170411757561 0.0 0.0 0.0012146240738491437 0.0 190-194 90.9170411757561 0.0 0.0 0.0012146240738491437 0.0 195-199 90.9170411757561 0.0 0.0 0.0012146240738491437 0.0 200-204 90.9170411757561 0.0 0.0 0.0012146240738491437 0.0 205-209 90.91801287501517 0.0 0.0 0.0012146240738491437 0.0 210-214 90.91825579982995 0.0 0.0 0.0012146240738491437 0.0 215-219 90.91825579982995 0.0 0.0 0.0014575488886189724 0.0 220-224 90.91825579982995 0.0 0.0 0.0024292481476982874 0.0 225-229 90.91825579982995 0.0 0.0 0.0024292481476982874 0.0 230-234 90.91825579982995 0.0 0.0 0.0024292481476982874 0.0 235-239 90.91825579982995 0.0 0.0 0.0024292481476982874 0.0 240-244 90.91825579982995 0.0 0.0 0.0024292481476982874 0.0 245-249 90.91825579982995 0.0 0.0 0.0024292481476982874 0.0 250-254 90.91825579982995 0.0 0.0 0.0024292481476982874 0.0 255-259 90.91825579982995 0.0 0.0 0.0024292481476982874 0.0 260-264 90.91825579982995 0.0 0.0 0.0024292481476982874 0.0 265-269 90.91825579982995 0.0 0.0 0.0024292481476982874 0.0 270-274 90.91825579982995 0.0 0.0 0.0024292481476982874 0.0 275-279 90.91825579982995 0.0 0.0 0.0024292481476982874 0.0 280-284 90.91825579982995 0.0 0.0 0.0024292481476982874 0.0 285-289 90.91825579982995 0.0 0.0 0.0024292481476982874 0.0 290 90.9194704239038 0.0 0.0 0.0036438722215474313 0.0 >>END_MODULE