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Updated Hisat2 Software. Temporarily transferring precompiled softwar…
…e package to Compute nodes instead of using OASIS module
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task-files/hisat2/hisat2-2.0.4-Linux_x86_64.zip
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tools/hisat2
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@@ -3,8 +3,6 @@ |
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set -e
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module load samtools/1.3.1
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-module load bowtie/2.2.9
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-module load hisat2/2.0.3-beta
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module load java/8u25
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set -v
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@@ -38,7 +36,9 @@ if [ $LEFT_SIZE -lt 50 -o $RIGHT_SIZE -lt 50 ]; then |
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exit 0
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fi
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-hisat2 --no-temp-splicesite --known-splicesite-infile ./$REF_PREFIX.Splice_Sites.txt -x ./$REF_PREFIX -q -1 ./left_result.paired_trim.forward_$COMMON_NAME.fastq -2 ./right_result.paired_trim.reverse_$COMMON_NAME.fastq -S $BASE_NAME-$COMMON_NAME.hits.sam -t --dta-cufflinks 2>&1 | tee $BASE_NAME-$COMMON_NAME-out.txt
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+unzip hisat2-2.0.4-Linux_x86_64.zip
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+
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+hisat2-2.0.4/hisat2 --no-temp-splicesite --known-splicesite-infile ./$REF_PREFIX.Splice_Sites.txt -x ./$REF_PREFIX -q -1 ./left_result.paired_trim.forward_$COMMON_NAME.fastq -2 ./right_result.paired_trim.reverse_$COMMON_NAME.fastq -S $BASE_NAME-$COMMON_NAME.hits.sam -t --dta-cufflinks 2>&1 | tee $BASE_NAME-$COMMON_NAME-out.txt
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# we only need the accepted hits, but it needs a unique name
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mv $BASE_NAME-$COMMON_NAME.hits.sam $BASE_NAME-$COMMON_NAME-accepted_hits.sam
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