diff --git a/healthcare/healthcare/doctype/lab_test_template/lab_test_template.py b/healthcare/healthcare/doctype/lab_test_template/lab_test_template.py
index a75c533f94..22bcfd808b 100644
--- a/healthcare/healthcare/doctype/lab_test_template/lab_test_template.py
+++ b/healthcare/healthcare/doctype/lab_test_template/lab_test_template.py
@@ -10,6 +10,7 @@
 from frappe import _
 from frappe.model.document import Document
 from frappe.model.rename_doc import rename_doc
+from frappe.utils import flt
 
 
 class LabTestTemplate(Document):
@@ -21,6 +22,9 @@ def validate(self):
 		if self.is_billable and (not self.lab_test_rate or self.lab_test_rate <= 0.0):
 			frappe.throw(_('Standard Selling Rate should be greater than zero.'))
 
+		if self.sample and flt(self.sample_qty) <= 0:
+			frappe.throw(_('Sample Quantity cannot be negative or 0'), title=_('Invalid Quantity'))
+
 		self.validate_conversion_factor()
 		self.enable_disable_item()