diff --git a/science/afni/Makefile b/science/afni/Makefile
index 495e26eeeaac6..ec6171b697d41 100644
--- a/science/afni/Makefile
+++ b/science/afni/Makefile
@@ -1,6 +1,6 @@
 PORTNAME=		afni
 DISTVERSIONPREFIX=	AFNI_
-DISTVERSION=		24.0.19
+DISTVERSION=		24.1.00
 CATEGORIES=		science biology graphics perl5 python
 
 MAINTAINER=	fernape@FreeBSD.org
diff --git a/science/afni/distinfo b/science/afni/distinfo
index 93c82aa907076..cec9e33ff1d09 100644
--- a/science/afni/distinfo
+++ b/science/afni/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1711905308
-SHA256 (afni-afni-AFNI_24.0.19_GH0.tar.gz) = 224c9ac920687be5867575ba1ce0df2ee971d4c9cb548de9653fbf6144ffde90
-SIZE (afni-afni-AFNI_24.0.19_GH0.tar.gz) = 52955739
+TIMESTAMP = 1712128705
+SHA256 (afni-afni-AFNI_24.1.00_GH0.tar.gz) = e9f86254cb39c28e38a65a67fd136a05e128e4c1d0bafe74bf595b504bbd9fbd
+SIZE (afni-afni-AFNI_24.1.00_GH0.tar.gz) = 52956946
diff --git a/science/afni/pkg-plist b/science/afni/pkg-plist
index e5c7678278283..69c968d86153b 100644
--- a/science/afni/pkg-plist
+++ b/science/afni/pkg-plist
@@ -590,6 +590,7 @@ bin/get_afni_model_PRF
 bin/get_afni_model_PRF_6
 bin/get_afni_model_PRF_6_BAD
 bin/gifti_tool
+bin/gtkyd_check
 bin/help_format
 bin/im2niml
 bin/images_equal