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Jetstream: Fails RNA-STAR 2.7.5b #306

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jennaj opened this issue Sep 15, 2020 · 4 comments
Closed

Jetstream: Fails RNA-STAR 2.7.5b #306

jennaj opened this issue Sep 15, 2020 · 4 comments

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@jennaj
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@jennaj jennaj commented Sep 15, 2020

Galaxy Tool ID: toolshed.g2.bx.psu.edu/repos/iuc/rgrnastar/rna_star/2.7.5b

Test history. Includes both mouse and human genome targets. hg19 failed -- and it was thought to have CVMFS indexes correct for this tool already (mouse not yet). Maybe some other problem?

Example error: data 13-15 (individual paired-end RNA-seq reads, no GTF, target genome hg19)

slurmstepd: error: couldn't chdir to `/srv/pulsar/main/venv/lib/python3.6/site-packages': No such file or directory: going to /tmp instead

EXITING because of FATAL ERROR: could not open genome file /cvmfs/data.galaxyproject.org/managed/rnastar/2.7.4a/hg19/hg19/dataset_e434ec00-2efb-4ecd-bf63-878223a9c4d4_files//genomeParameters.txt
SOLUTION: check that the path to genome files, specified in --genomeDir is correct and the files are present, and have user read permsissions

Sep 14 20:42:03 ...... FATAL ERROR, exiting
[E::hts_open_format] Failed to open file "/jetstream/scratch0/main/jobs/30602736/outputs/dataset_45122796.dat" : No such file or directory
[E::hts_open_format] Failed to open file "/jetstream/scratch0/main/jobs/30602736/outputs/dataset_45122796.dat" : No such file or directory

Reported in bug reports and at Galaxy Help: https://help.galaxyproject.org/t/another-star-error/4442

cc @natefoo @davebx

@jennaj jennaj added this to Auto Preset: To do Newly Added in Bugs being fixed via automation Sep 15, 2020
@jennaj
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@jennaj jennaj commented Sep 15, 2020

Priority genomes as part of #292 (closed) and #266 (current)

hg38
hg19
mm10
mm9
rn6
dm6
dm3
ce10

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@jennaj
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@jennaj jennaj commented Sep 15, 2020

"9/14/20 Tests" status

9/15/20 noon

  1. hg38 -- success! individual inputs or collection -- this index is correct (expected)
  2. hg19 -- fails all -- this index is not corrected yet or at least not when accessed from jetstream (unexpected)
  3. mm9 -- fails individual inputs (collection queued) -- this index (and mm10) are pending the known correction (expected)

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@jennaj jennaj assigned davebx and natefoo and unassigned davebx Sep 15, 2020
@jennaj jennaj moved this from Auto Preset: To do Newly Added to WIP in Bugs being fixed Sep 15, 2020
@jennaj jennaj moved this from WIP to Priority in Bugs being fixed Sep 15, 2020
@jennaj
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@jennaj jennaj commented Sep 25, 2020

Tool restricted to hg38 -- the only currently active working index for this tool version.

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@jennaj
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@jennaj jennaj commented Sep 30, 2020

More genomes indexed and working.
More will be added.
As those become available, each will appear on the tool form.

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@jennaj jennaj closed this Sep 30, 2020
Bugs being fixed automation moved this from Priority to Auto Preset: Done Closed Sep 30, 2020
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