diff --git a/CHANGELOG.md b/CHANGELOG.md
index 691981868c..df138d36ea 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -2,6 +2,10 @@
 
 ## Unreleased
 
+### Fixes
+
+- Fixed null IServiceProvider in anonymous routes with OpenTelemetry ([#3401](https://github.com/getsentry/sentry-dotnet/pull/3401))
+
 ### Dependencies
 
 - Bump CLI from v2.31.2 to v2.32.1 ([#3398](https://github.com/getsentry/sentry-dotnet/pull/3398))
diff --git a/src/Sentry.AspNetCore/ScopeExtensions.cs b/src/Sentry.AspNetCore/ScopeExtensions.cs
index a8e33677a7..dcddf56ded 100644
--- a/src/Sentry.AspNetCore/ScopeExtensions.cs
+++ b/src/Sentry.AspNetCore/ScopeExtensions.cs
@@ -38,7 +38,10 @@ public static void Populate(this Scope scope, HttpContext context, SentryAspNetC
 
         if (options.SendDefaultPii && !scope.HasUser())
         {
-            var userFactory = context.RequestServices.GetService<ISentryUserFactory>();
+            // Although the compiler suggests context.RequestServices cannot be null, in practice it can be, so we
+            // have a null check here.
+            // See: https://github.com/getsentry/sentry-dotnet/issues/3395
+            var userFactory = context.RequestServices?.GetService<ISentryUserFactory>();
             var user = userFactory?.Create();
 
             if (user != null)