diff --git a/.github/.OwlBot.lock.yaml b/.github/.OwlBot.lock.yaml
index 51b21a62b..4a311db02 100644
--- a/.github/.OwlBot.lock.yaml
+++ b/.github/.OwlBot.lock.yaml
@@ -13,5 +13,5 @@
 # limitations under the License.
 docker:
   image: gcr.io/cloud-devrel-public-resources/owlbot-python:latest
-  digest: sha256:a7aef70df5f13313ddc027409fc8f3151422ec2a57ac8730fce8fa75c060d5bb
-# created: 2025-04-10T17:00:10.042601326Z
+  digest: sha256:543e209e7c1c1ffe720eb4db1a3f045a75099304fb19aa11a47dc717b8aae2a9
+# created: 2025-10-09T14:48:42.914384887Z
diff --git a/.kokoro/samples/python3.14/common.cfg b/.kokoro/samples/python3.14/common.cfg
new file mode 100644
index 000000000..a083385d7
--- /dev/null
+++ b/.kokoro/samples/python3.14/common.cfg
@@ -0,0 +1,40 @@
+# Format: //devtools/kokoro/config/proto/build.proto
+
+# Build logs will be here
+action {
+  define_artifacts {
+    regex: "**/*sponge_log.xml"
+  }
+}
+
+# Specify which tests to run
+env_vars: {
+    key: "RUN_TESTS_SESSION"
+    value: "py-3.14"
+}
+
+# Declare build specific Cloud project.
+env_vars: {
+    key: "BUILD_SPECIFIC_GCLOUD_PROJECT"
+    value: "python-docs-samples-tests-314"
+}
+
+env_vars: {
+    key: "TRAMPOLINE_BUILD_FILE"
+    value: "github/python-api-core/.kokoro/test-samples.sh"
+}
+
+# Configure the docker image for kokoro-trampoline.
+env_vars: {
+    key: "TRAMPOLINE_IMAGE"
+    value: "gcr.io/cloud-devrel-kokoro-resources/python-samples-testing-docker"
+}
+
+# Download secrets for samples
+gfile_resources: "/bigstore/cloud-devrel-kokoro-resources/python-docs-samples"
+
+# Download trampoline resources.
+gfile_resources: "/bigstore/cloud-devrel-kokoro-resources/trampoline"
+
+# Use the trampoline script to run in docker.
+build_file: "python-api-core/.kokoro/trampoline_v2.sh"
diff --git a/.kokoro/samples/python3.14/continuous.cfg b/.kokoro/samples/python3.14/continuous.cfg
new file mode 100644
index 000000000..a1c8d9759
--- /dev/null
+++ b/.kokoro/samples/python3.14/continuous.cfg
@@ -0,0 +1,6 @@
+# Format: //devtools/kokoro/config/proto/build.proto
+
+env_vars: {
+    key: "INSTALL_LIBRARY_FROM_SOURCE"
+    value: "True"
+}
\ No newline at end of file
diff --git a/.kokoro/samples/python3.14/periodic-head.cfg b/.kokoro/samples/python3.14/periodic-head.cfg
new file mode 100644
index 000000000..a18c0cfc6
--- /dev/null
+++ b/.kokoro/samples/python3.14/periodic-head.cfg
@@ -0,0 +1,11 @@
+# Format: //devtools/kokoro/config/proto/build.proto
+
+env_vars: {
+    key: "INSTALL_LIBRARY_FROM_SOURCE"
+    value: "True"
+}
+
+env_vars: {
+    key: "TRAMPOLINE_BUILD_FILE"
+    value: "github/python-api-core/.kokoro/test-samples-against-head.sh"
+}
diff --git a/.kokoro/samples/python3.14/periodic.cfg b/.kokoro/samples/python3.14/periodic.cfg
new file mode 100644
index 000000000..71cd1e597
--- /dev/null
+++ b/.kokoro/samples/python3.14/periodic.cfg
@@ -0,0 +1,6 @@
+# Format: //devtools/kokoro/config/proto/build.proto
+
+env_vars: {
+    key: "INSTALL_LIBRARY_FROM_SOURCE"
+    value: "False"
+}
diff --git a/.kokoro/samples/python3.14/presubmit.cfg b/.kokoro/samples/python3.14/presubmit.cfg
new file mode 100644
index 000000000..a1c8d9759
--- /dev/null
+++ b/.kokoro/samples/python3.14/presubmit.cfg
@@ -0,0 +1,6 @@
+# Format: //devtools/kokoro/config/proto/build.proto
+
+env_vars: {
+    key: "INSTALL_LIBRARY_FROM_SOURCE"
+    value: "True"
+}
\ No newline at end of file