2018/12/17 18:27:33 reflow version 0.6.3 (go1.10) 2018/12/17 18:27:33 run ID: e3de9706 2018/12/17 18:27:33 evaluating program /home/ubuntu/fusion_detection_pipeline/reflow/fusionDetectionReflow.rf params: input_fq1=s3://darmanis-group/singlecell_lungadeno/non_immune/nonImmune_fastqs_9.27/170125/A10_1001000293/A10_1001000293_R1_001.fastq.gz input_fq2=s3://darmanis-group/singlecell_lungadeno/non_immune/nonImmune_fastqs_9.27/170125/A10_1001000293/A10_1001000293_R2_001.fastq.gz output_dir=s3://s3://darmanis-group/singlecell_lungadeno/non_immune/nonImmune_fastqs_9.27/170125/A10_1001000293/testOut/ sample_id=A10_1001000293 threads=8 (no arguments) 2018/12/17 18:27:34 ec2cluster: pending{} 2018/12/17 18:27:34 ec2cluster: pending{} 2018/12/17 18:27:35 ec2cluster: allocate {mem:64.0GiB cpu:16 disk:1000.0GiB} 2018/12/17 18:27:35 ec2cluster: attempting to allocate from existing pool 2018/12/17 18:27:35 ec2cluster: failed to allocate from existing pool: unavailable: no allocs available in pool; provisioning from EC2 2018/12/17 18:27:35 ec2cluster: pending{} waiter0{mem:64.0GiB cpu:16 disk:1000.0GiB} 2018/12/17 18:27:35 ec2cluster: launch c5.9xlarge{mem:67.0GiB cpu:36 disk:2.4TiB intel_avx:36 intel_avx2:36 intel_avx512:36} pending{mem:67.0GiB cpu:36 disk:2.4TiB intel_avx:36 intel_avx2:36 intel_avx512:36} 2018/12/17 18:27:35 ec2cluster: EC2RunInstances { BlockDeviceMappings: [{ DeviceName: "/dev/xvda", Ebs: { DeleteOnTermination: true, VolumeSize: 200, VolumeType: "gp2" } },{ DeviceName: "/dev/xvdb", Ebs: { DeleteOnTermination: true, VolumeSize: 2500, VolumeType: "gp2" } }], ClientToken: "96aa55abbd650205", DisableApiTermination: false, DryRun: false, EbsOptimized: true, IamInstanceProfile: { Arn: "" }, ImageId: "ami-7c488704", InstanceInitiatedShutdownBehavior: "terminate", InstanceType: "c5.9xlarge", MaxCount: 1, MinCount: 1, Monitoring: { Enabled: true }, 2018/12/17 18:27:36 ec2cluster: launched instance i-0bae0238ad1b98c87: c5.9xlarge{mem:67.0GiB cpu:36 disk:2.4TiB intel_avx:36 intel_avx2:36 intel_avx512:36} 2018/12/17 18:28:33 ec2cluster: added instance c5.9xlarge resources{mem:67.0GiB cpu:36 disk:2.4TiB intel_avx:36 intel_avx2:36 intel_avx512:36} pending{} available{mem:3.0GiB cpu:20 disk:1.5TiB intel_avx:36 intel_avx2:36 intel_avx512:36} npending:0 waiters:0 notified:1 2018/12/17 18:28:33 ec2cluster: pending{} 2018/12/17 18:28:33 accepted alloc ec2-52-24-239-24.us-west-2.compute.amazonaws.com:9000/9fff6e6847880d39 2018/12/17 18:28:33 run state: eval alloc ec2-52-24-239-24.us-west-2.compute.amazonaws.com:9000/9fff6e6847880d39 2018/12/17 18:28:33 evaluating with configuration: executor *client.clientAlloc transferer *repository.Manager flags cacheextern,cacheread,cachewrite,nogc,norecomputeempty,topdown flowconfig hashv2 cachelookuptimeout 1m0s 2018/12/17 18:28:33 assoc.Get flow 09d955ef state FlowLookup {mem:64.0GiB cpu:8 disk:1000.0GiB} exec image trinityctat/ctatfusion cmd "\n\t\t/usr/local/src/STAR-Fusion/STAR-Fusion --left_fq %s/A10_1001000293_R1_001.fastq.gz --right_fq %s/A10_1001000293_R2_001.fastq.gz --genome_lib_dir %s/GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ -O %s --FusionInspector validate --examine_coding_effect --denovo_reconstruct --CPU 8\n\t" deps 2826e73f,2826e73f,0bd4d6cb: ResourceNotFoundException: Requested resource not found status code: 400, request id: 397JC79LEH1A0PA86RQLBP3UEBVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:28:33 assoc.Get flow dbd7f9ea state FlowLookup {} intern url "s3://darmanis-group/singlecell_lungadeno/non_immune/nonImmune_fastqs_9.27/170125/A10_1001000293/A10_1001000293_R2_001.fastq.gz": ResourceNotFoundException: Requested resource not found status code: 400, request id: 46OGR9HH1T8SFU634GD6K44A2BVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:28:33 assoc.Get flow 624d8a66 state FlowLookup {} intern url "s3://darmanis-group/singlecell_lungadeno/non_immune/nonImmune_fastqs_9.27/170125/A10_1001000293/A10_1001000293_R1_001.fastq.gz": ResourceNotFoundException: Requested resource not found status code: 400, request id: ST2MNUBQ9C6SM4JSBQQUM9S1HRVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:28:33 assoc.Get flow 0936f2b1 state FlowLookup {mem:8.0GiB cpu:8 disk:64.0GiB} exec image trinityctat/ctatfusion cmd " \n\t\ttar xvf %s -C %s\n\t" deps bf2ba2ec: ResourceNotFoundException: Requested resource not found status code: 400, request id: SP7S1U99IIQPD6M2JR19K7UM0JVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:28:33 assoc.Get flow 24f7fd3f state FlowLookup {} intern url "s3://darmanis-group/singlecell_lungadeno/GRCh37_v19_CTAT_lib_Feb092018.plug-n-play.tar.gz": ResourceNotFoundException: Requested resource not found status code: 400, request id: 2JMK8KRDE5L8TKMGPL5RK5855VVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:28:33 -> fusionDetectionReflow.fq2 dbd7f9ea run intern .._9.27/170125/A10_1001000293/A10_1001000293_R2_001.fastq.gz 2018/12/17 18:28:33 fusionDetectionReflow.fq2 dbd7f9ea /home/ubuntu/fusion_detection_pipeline/reflow/fusionDetectionReflow.rf:59:15: sha256:dbd7f9ea44a2ed984bac7249db60da7c673fe8b8f4bd55fe9a2d4d47f9ee5023 2018/12/17 18:28:33 -> fusionDetectionReflow.fq1 624d8a66 run intern .._9.27/170125/A10_1001000293/A10_1001000293_R1_001.fastq.gz 2018/12/17 18:28:33 fusionDetectionReflow.fq1 624d8a66 /home/ubuntu/fusion_detection_pipeline/reflow/fusionDetectionReflow.rf:58:15: sha256:624d8a661399f1688d670300b09a242db16bdc1c9f4be81b4e68e5206b8c94ab 2018/12/17 18:28:33 -> fusionDetectionReflow.gBuildComp 24f7fd3f run intern ..lungadeno/GRCh37_v19_CTAT_lib_Feb092018.plug-n-play.tar.gz 2018/12/17 18:28:33 fusionDetectionReflow.gBuildComp 24f7fd3f /home/ubuntu/fusion_detection_pipeline/reflow/fusionDetectionReflow.rf:61:22: sha256:24f7fd3f027a7d8cfaf6a066a903f6c3dfc3b0ca3d74b1a6843cdf54615aaf08 2018/12/17 18:28:33 <- fusionDetectionReflow.fq2 dbd7f9ea ok intern 0s 16.7KiB 2018/12/17 18:28:33 fusionDetectionReflow.fq2 dbd7f9ea /home/ubuntu/fusion_detection_pipeline/reflow/fusionDetectionReflow.rf:59:15: sha256:dbd7f9ea44a2ed984bac7249db60da7c673fe8b8f4bd55fe9a2d4d47f9ee5023 result: . sha256:07b7204847038de3e8c337ffffe3db06f270d29e18e59e93f2f1e048155e5104 16.7KiB 2018/12/17 18:28:33 <- fusionDetectionReflow.fq1 624d8a66 ok intern 0s 16.5KiB 2018/12/17 18:28:33 fusionDetectionReflow.fq1 624d8a66 /home/ubuntu/fusion_detection_pipeline/reflow/fusionDetectionReflow.rf:58:15: sha256:624d8a661399f1688d670300b09a242db16bdc1c9f4be81b4e68e5206b8c94ab result: . sha256:7fe8583438a0925e2f308f318df37f384f3fe6925a3b515d4c986a1881a69b58 16.5KiB 2018/12/17 18:28:33 cache write flow dbd7f9ea state FlowDone {} intern url "s3://darmanis-group/singlecell_lungadeno/non_immune/nonImmune_fastqs_9.27/170125/A10_1001000293/A10_1001000293_R2_001.fastq.gz": ResourceNotFoundException: Requested resource not found status code: 400, request id: 7H0QG512PC5SGUDC2PMOMBNS6FVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:28:33 cache write flow 624d8a66 state FlowDone {} intern url "s3://darmanis-group/singlecell_lungadeno/non_immune/nonImmune_fastqs_9.27/170125/A10_1001000293/A10_1001000293_R1_001.fastq.gz": ResourceNotFoundException: Requested resource not found status code: 400, request id: H97OPLSPMDKHDV7V3EEANBN2NJVV4KQNSO5AEMVJF66Q9ASUAAJG ec2cluster: 2 instances: c5.9xlarge:2 (<=$3.1/hr), total{mem:133.9GiB cpu:72 disk:4.9TiB intel_avx:72 intel_avx2:72 intel_avx512:72}, waiting{}, pending{} allocate {mem:64.0GiB cpu:16 disk:1000.0GiB}: alloc ec2-52-24-239-24.us-west-2.compute.amazonaws.com:9000/9fff6e6847880d39 58s i-0bae0238ad1b98c87: instance ready 58s transfers: e3de9706: fusionDetectionReflow.fq2: intern done 16.7KiB 0s fusionDetectionReflow.fq1: intern done 16.5KiB 0s fusionDetectionReflow.gBuildComp: intern s3://darmanis-group/singlecell_lungadeno/GRCh37_v19_CTAT_lib_Feb092018.plug-n-play.tar.gz 0s 2018/12/17 18:28:34 ec2cluster: pending{} ec2cluster: 2 instances: c5.9xlarge:2 (<=$3.1/hr), total{mem:133.9GiB cpu:72 disk:4.9TiB intel_avx:72 intel_avx2:72 intel_avx512:72}, waiting{}, pending{} transfers: e3de9706: elapsed: 1m0s, running:1, completed: 2/5 fusionDetectionReflow.gBuildComp: intern s3://darmanis-group/singlecell_lungadeno/GRCh37_v19_CTAT_lib_Feb092018.plug-n-play.tar.gz 1m0s 2018/12/17 18:29:34 ec2cluster: pending{} ec2cluster: 2 instances: c5.9xlarge:2 (<=$3.1/hr), total{mem:133.9GiB cpu:72 disk:4.9TiB intel_avx:72 intel_avx2:72 intel_avx512:72}, waiting{}, pending{} transfers: e3de9706: elapsed: 2m0s, running:1, completed: 2/5 fusionDetectionReflow.gBuildComp: intern s3://darmanis-group/singlecell_lungadeno/GRCh37_v19_CTAT_lib_Feb092018.plug-n-play.tar.gz 2m0s 2018/12/17 18:30:34 ec2cluster: pending{} 2018/12/17 18:31:10 <- fusionDetectionReflow.gBuildComp 24f7fd3f ok intern 0s 27.5GiB 2018/12/17 18:31:10 fusionDetectionReflow.gBuildComp 24f7fd3f /home/ubuntu/fusion_detection_pipeline/reflow/fusionDetectionReflow.rf:61:22: sha256:24f7fd3f027a7d8cfaf6a066a903f6c3dfc3b0ca3d74b1a6843cdf54615aaf08 result: . sha256:53bdcbdd1d203acb599aac5a7abfecdc2fe6719d62f1faf75327f4046dc4402e 27.5GiB 2018/12/17 18:31:10 -> fusionDetectionReflow.Decompress 0936f2b1 run exec trinityctat/ctatfusion tar xvf {{tgz}} -C {{output}} 2018/12/17 18:31:10 fusionDetectionReflow.Decompress 0936f2b1 /home/ubuntu/fusion_detection_pipeline/reflow/fusionDetectionReflow.rf:43:6: resources: {mem:8.0GiB cpu:8 disk:64.0GiB} sha256:308b33c34c2a39436f9dfc569da73edd53f1a48e47d9db06f71fe7dc474372b6 sha256:0936f2b16e141cd20f0768d683b6447460a0357f1d1cfc6ea91e65b992295f61 ec2-52-24-239-24.us-west-2.compute.amazonaws.com:9000/9fff6e6847880d39/0936f2b16e141cd20f0768d683b6447460a0357f1d1cfc6ea91e65b992295f61 trinityctat/ctatfusion command: tar xvf {{tgz}} -C {{output}} where: {{tgz}} = . sha256:53bdcbdd1d203acb599aac5a7abfecdc2fe6719d62f1faf75327f4046dc4402e 27.5GiB 2018/12/17 18:31:11 cache write flow 24f7fd3f state FlowDone {} intern url "s3://darmanis-group/singlecell_lungadeno/GRCh37_v19_CTAT_lib_Feb092018.plug-n-play.tar.gz": ResourceNotFoundException: Requested resource not found status code: 400, request id: BO6C51DG2SV6NMSF82G21S9IRFVV4KQNSO5AEMVJF66Q9ASUAAJG ec2cluster: 2 instances: c5.9xlarge:2 (<=$3.1/hr), total{mem:133.9GiB cpu:72 disk:4.9TiB intel_avx:72 intel_avx2:72 intel_avx512:72}, waiting{}, pending{} transfers: e3de9706: elapsed: 3m0s, running:1, completed: 3/5 fusionDetectionReflow.Decompress: exec trinityctat/ctatfusion tar xvf {{tgz}} -C {{output}} 22s 2018/12/17 18:31:34 ec2cluster: pending{} ec2cluster: 2 instances: c5.9xlarge:2 (<=$3.1/hr), total{mem:133.9GiB cpu:72 disk:4.9TiB intel_avx:72 intel_avx2:72 intel_avx512:72}, waiting{}, pending{} transfers: e3de9706: elapsed: 4m0s, running:1, completed: 3/5 fusionDetectionReflow.Decompress: exec trinityctat/ctatfusion tar xvf {{tgz}} -C {{output}} 1m22s 2018/12/17 18:32:34 ec2cluster: pending{} ec2cluster: 2 instances: c5.9xlarge:2 (<=$3.1/hr), total{mem:133.9GiB cpu:72 disk:4.9TiB intel_avx:72 intel_avx2:72 intel_avx512:72}, waiting{}, pending{} transfers: e3de9706: elapsed: 5m0s, running:1, completed: 3/5 fusionDetectionReflow.Decompress: exec trinityctat/ctatfusion tar xvf {{tgz}} -C {{output}} 2m22s 2018/12/17 18:33:34 ec2cluster: pending{} ec2cluster: 2 instances: c5.9xlarge:2 (<=$3.1/hr), total{mem:133.9GiB cpu:72 disk:4.9TiB intel_avx:72 intel_avx2:72 intel_avx512:72}, waiting{}, pending{} transfers: e3de9706: elapsed: 6m0s, running:1, completed: 3/5 fusionDetectionReflow.Decompress: exec trinityctat/ctatfusion tar xvf {{tgz}} -C {{output}} 3m22s 2018/12/17 18:34:34 ec2cluster: pending{} ec2cluster: 2 instances: c5.9xlarge:2 (<=$3.1/hr), total{mem:133.9GiB cpu:72 disk:4.9TiB intel_avx:72 intel_avx2:72 intel_avx512:72}, waiting{}, pending{} transfers: e3de9706: elapsed: 7m0s, running:1, completed: 3/5 fusionDetectionReflow.Decompress: exec trinityctat/ctatfusion tar xvf {{tgz}} -C {{output}} 4m22s 2018/12/17 18:35:34 ec2cluster: pending{} ec2cluster: 2 instances: c5.9xlarge:2 (<=$3.1/hr), total{mem:133.9GiB cpu:72 disk:4.9TiB intel_avx:72 intel_avx2:72 intel_avx512:72}, waiting{}, pending{} transfers: e3de9706: elapsed: 8m0s, running:1, completed: 3/5 fusionDetectionReflow.Decompress: exec trinityctat/ctatfusion tar xvf {{tgz}} -C {{output}} 5m22s 2018/12/17 18:36:34 ec2cluster: pending{} ec2cluster: 2 instances: c5.9xlarge:2 (<=$3.1/hr), total{mem:133.9GiB cpu:72 disk:4.9TiB intel_avx:72 intel_avx2:72 intel_avx512:72}, waiting{}, pending{} transfers: e3de9706: elapsed: 9m0s, running:1, completed: 3/5 fusionDetectionReflow.Decompress: exec trinityctat/ctatfusion tar xvf {{tgz}} -C {{output}} 6m22s 2018/12/17 18:37:34 ec2cluster: pending{} ec2cluster: 2 instances: c5.9xlarge:2 (<=$3.1/hr), total{mem:133.9GiB cpu:72 disk:4.9TiB intel_avx:72 intel_avx2:72 intel_avx512:72}, waiting{}, pending{} transfers: e3de9706: elapsed: 10m, running:1, completed: 3/5 fusionDetectionReflow.Decompress: exec trinityctat/ctatfusion tar xvf {{tgz}} -C {{output}} 7m22s 2018/12/17 18:38:34 ec2cluster: pending{} 2018/12/17 18:38:41 <- fusionDetectionReflow.Decompress 0936f2b1 ok exec 4m3s 41.6GiB 2018/12/17 18:38:41 fusionDetectionReflow.Decompress 0936f2b1 /home/ubuntu/fusion_detection_pipeline/reflow/fusionDetectionReflow.rf:43:6: resources: {mem:8.0GiB cpu:8 disk:64.0GiB} sha256:308b33c34c2a39436f9dfc569da73edd53f1a48e47d9db06f71fe7dc474372b6 sha256:0936f2b16e141cd20f0768d683b6447460a0357f1d1cfc6ea91e65b992295f61 ec2-52-24-239-24.us-west-2.compute.amazonaws.com:9000/9fff6e6847880d39/0936f2b16e141cd20f0768d683b6447460a0357f1d1cfc6ea91e65b992295f61 trinityctat/ctatfusion command: tar xvf {{tgz}} -C {{output}} where: {{tgz}} = . sha256:53bdcbdd1d203acb599aac5a7abfecdc2fe6719d62f1faf75327f4046dc4402e 27.5GiB result: {{output}} = GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/AnnotFilterRule.pm sha256:a57af06c37f03f1daf9f58fa4906990192eb303a3792130185fca3d6ffcef9cb 2.8KiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/blast_pairs.idx sha256:537cd37329c03079040c2df23229f438f29c8e4cf7c033410ccbad45001d5c0e 1.1GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/blast_pairs.idx.prev.1518210799 sha256:393503c7547092e3f5527a23dfec738ed298559c68c304132826152f8222e87d 1.1GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/fusion_annot_lib.idx sha256:76f709ad7373feac1127707113026f36d1c29d77b6026382769dd6e506f7ece0 2.2GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/pfam_domains.dbm sha256:ba8afc36d70491dedb53e8dbdf7b991b0433cd894824ea1061e024fcd9e429ab 191.7MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.cds sha256:65d7b76c1f0c9dc79aa582e32153263d2904a85f3290b58a71382ebb08cbaeab 105.4MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.gtf sha256:b74409636f142a951c1cbc578b45950fb299575ac00ce25c90bce85a26483f66 1.1GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans sha256:45476e5c66d7a269fae32a77004e945345057ec85dd33c3a23bc8f31a3c77532 3.6MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu sha256:255a753ae422ea9e332df9ccafbe734420ab93add8b9b10e3860c90266bbf64b 47.8MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.pep sha256:0409e25d842f922cd408ed49f8983369e2877648b42059ad2ff58b91e22f6995 36.3MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm sha256:62139d0941a7c2a586834520c5b8704810426a9a48a6c0a3c84a5706acf075ff 695.1MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa sha256:7d517a1e1c1a05e0b5ee4af5da0aa11faecac7f2fbd89e98da627be399909f94 2.9GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.fai sha256:a3b9a0dc4fcdac8e1a8a66c23ac57c16e6195129f76742d313878c7c11686388 788B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.gmap.ok sha256:e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855 0B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome sha256:99790df229ca81fdecfdef3ad28dec074e87b7f160b2d01105a9f87b3b2764be 3.0GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA sha256:f7bfab0be699f4bfee75680d2c8903831a23a479c8bf7a45b522e567f5b6aa94 22.8GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex sha256:80915c6510346ad234b2f6e1e1af8d0d2ce73822325462aaa85db18ceadb58e3 1.5GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok sha256:e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855 0B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt sha256:95a6714767439d1cc12783914de176b40c572a9e58c0ba123c1a6cc464873d96 238B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt sha256:551419732b876288523ed180e3d2483f101b1ad25ff0c02bcb4b0933364924de 138B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt sha256:da0b1d6f35f95e157c3859967941a34e102943b691375048f1c7268a293d4786 376B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt sha256:21306aa9471cd586fed584b2daadb4613b487ecbfae954b3edd445282cacd217 273B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab sha256:b9b766738477a4f5c52c3755d2bb1e936b632ceb1d42a4b37e6554ed53a1c9fb 40.5MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab sha256:d211c58b7d6f5c9325871a8dd56ee77df253c4f6d17a840e691dc1af9fa16f81 16.4MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab sha256:f837e5346a93778d27986b8373d0e26cf85a5efef4d45b60d0d4823c58e8d80d 1022.0KiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt sha256:0456aa53abecc7301a93f57866067fba50d6a97e2c59623e6bf3e59d8d67cba2 1.0KiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt sha256:baf9fcf0cce8d51548f6db7b02e71025a5df955bdf43e6429bf35faffb73755a 9.6MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab sha256:4d3bdce14b5848014fd69393b9d419d2d5f5f783238ae0f61bdd305d8427073d 8.5MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab sha256:f28dce23bec790e520b256c4c1b92c6ef6145bfffc73f929082c19c06e555d40 8.5MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab sha256:1cad9fc7ebe9fe3a2b33640e1a0d80716aeb059e39fef136aa95cf05733320b3 11.5MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat sha256:72a954068b2c468985b806b729ebfe48295113884c774142adfd2151602eaebd 1.1GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm sha256:0437f3a2bcaad994b8d0625f44163c2d7f203724a006766981313680eab9a14b 3.6GiB profile: cpu mean=1.1 max=1.3 mem mean=52.4MiB max=99.5MiB disk mean=16.4GiB max=33.2GiB tmp mean=0B max=0B 2018/12/17 18:38:42 -> fusionDetectionReflow.StarFusion 09d955ef run exec trinityctat/ctatfusion ../STAR-Fusion --left_fq {{d}.. --denovo_reconstruct --CPU 8 2018/12/17 18:38:42 fusionDetectionReflow.StarFusion 09d955ef /home/ubuntu/fusion_detection_pipeline/reflow/fusionDetectionReflow.rf:53:6: resources: {mem:64.0GiB cpu:8 disk:1000.0GiB} sha256:cf034633c130f3154feab2589aec06fa2835ce3ae28badb947e5d4326a33bbab sha256:09d955efb8ffc57b4253512697372bd5cad710973008ff2dad76e064d248188a ec2-52-24-239-24.us-west-2.compute.amazonaws.com:9000/9fff6e6847880d39/09d955efb8ffc57b4253512697372bd5cad710973008ff2dad76e064d248188a trinityctat/ctatfusion command: /usr/local/src/STAR-Fusion/STAR-Fusion --left_fq {{d}}/A10_1001000293_R1_001.fastq.gz --right_fq {{d}}/A10_1001000293_R2_001.fastq.gz --genome_lib_dir {{gBuildDecomp}}/GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ -O {{outDir}} --FusionInspector validate --examine_coding_effect --denovo_reconstruct --CPU 8 where: {{d}} = A10_1001000293_R1_001.fastq.gz sha256:7fe8583438a0925e2f308f318df37f384f3fe6925a3b515d4c986a1881a69b58 16.5KiB A10_1001000293_R2_001.fastq.gz sha256:07b7204847038de3e8c337ffffe3db06f270d29e18e59e93f2f1e048155e5104 16.7KiB {{d}} = A10_1001000293_R1_001.fastq.gz sha256:7fe8583438a0925e2f308f318df37f384f3fe6925a3b515d4c986a1881a69b58 16.5KiB A10_1001000293_R2_001.fastq.gz sha256:07b7204847038de3e8c337ffffe3db06f270d29e18e59e93f2f1e048155e5104 16.7KiB {{gBuildDecomp}} = GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/AnnotFilterRule.pm sha256:a57af06c37f03f1daf9f58fa4906990192eb303a3792130185fca3d6ffcef9cb 2.8KiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/blast_pairs.idx sha256:537cd37329c03079040c2df23229f438f29c8e4cf7c033410ccbad45001d5c0e 1.1GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/blast_pairs.idx.prev.1518210799 sha256:393503c7547092e3f5527a23dfec738ed298559c68c304132826152f8222e87d 1.1GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/fusion_annot_lib.idx sha256:76f709ad7373feac1127707113026f36d1c29d77b6026382769dd6e506f7ece0 2.2GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/pfam_domains.dbm sha256:ba8afc36d70491dedb53e8dbdf7b991b0433cd894824ea1061e024fcd9e429ab 191.7MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.cds sha256:65d7b76c1f0c9dc79aa582e32153263d2904a85f3290b58a71382ebb08cbaeab 105.4MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.gtf sha256:b74409636f142a951c1cbc578b45950fb299575ac00ce25c90bce85a26483f66 1.1GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans sha256:45476e5c66d7a269fae32a77004e945345057ec85dd33c3a23bc8f31a3c77532 3.6MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu sha256:255a753ae422ea9e332df9ccafbe734420ab93add8b9b10e3860c90266bbf64b 47.8MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.pep sha256:0409e25d842f922cd408ed49f8983369e2877648b42059ad2ff58b91e22f6995 36.3MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm sha256:62139d0941a7c2a586834520c5b8704810426a9a48a6c0a3c84a5706acf075ff 695.1MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa sha256:7d517a1e1c1a05e0b5ee4af5da0aa11faecac7f2fbd89e98da627be399909f94 2.9GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.fai sha256:a3b9a0dc4fcdac8e1a8a66c23ac57c16e6195129f76742d313878c7c11686388 788B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.gmap.ok sha256:e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855 0B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome sha256:99790df229ca81fdecfdef3ad28dec074e87b7f160b2d01105a9f87b3b2764be 3.0GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA sha256:f7bfab0be699f4bfee75680d2c8903831a23a479c8bf7a45b522e567f5b6aa94 22.8GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex sha256:80915c6510346ad234b2f6e1e1af8d0d2ce73822325462aaa85db18ceadb58e3 1.5GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok sha256:e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855 0B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrLength.txt sha256:95a6714767439d1cc12783914de176b40c572a9e58c0ba123c1a6cc464873d96 238B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrName.txt sha256:551419732b876288523ed180e3d2483f101b1ad25ff0c02bcb4b0933364924de 138B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrNameLength.txt sha256:da0b1d6f35f95e157c3859967941a34e102943b691375048f1c7268a293d4786 376B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/chrStart.txt sha256:21306aa9471cd586fed584b2daadb4613b487ecbfae954b3edd445282cacd217 273B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonGeTrInfo.tab sha256:b9b766738477a4f5c52c3755d2bb1e936b632ceb1d42a4b37e6554ed53a1c9fb 40.5MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/exonInfo.tab sha256:d211c58b7d6f5c9325871a8dd56ee77df253c4f6d17a840e691dc1af9fa16f81 16.4MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/geneInfo.tab sha256:f837e5346a93778d27986b8373d0e26cf85a5efef4d45b60d0d4823c58e8d80d 1022.0KiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/genomeParameters.txt sha256:0456aa53abecc7301a93f57866067fba50d6a97e2c59623e6bf3e59d8d67cba2 1.0KiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbInfo.txt sha256:baf9fcf0cce8d51548f6db7b02e71025a5df955bdf43e6429bf35faffb73755a 9.6MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.fromGTF.out.tab sha256:4d3bdce14b5848014fd69393b9d419d2d5f5f783238ae0f61bdd305d8427073d 8.5MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/sjdbList.out.tab sha256:f28dce23bec790e520b256c4c1b92c6ef6145bfffc73f929082c19c06e555d40 8.5MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/transcriptInfo.tab sha256:1cad9fc7ebe9fe3a2b33640e1a0d80716aeb059e39fef136aa95cf05733320b3 11.5MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dat sha256:72a954068b2c468985b806b729ebfe48295113884c774142adfd2151602eaebd 1.1GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/trans.blast.align_coords.align_coords.dbm sha256:0437f3a2bcaad994b8d0625f44163c2d7f203724a006766981313680eab9a14b 3.6GiB 2018/12/17 18:38:42 assoc store execinspect: ResourceNotFoundException: Requested resource not found status code: 400, request id: JRA4IPJP0UN1ML12N4EP3DRPT7VV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:38:42 assoc store execinspect: ResourceNotFoundException: Requested resource not found status code: 400, request id: M5H7LFR5IM261TP1PAAEHNDSRNVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:38:42 assoc store stdout: ResourceNotFoundException: Requested resource not found status code: 400, request id: HT567DR5O0AU0N02IB3I6NFS7JVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:38:42 assoc store stdout: ResourceNotFoundException: Requested resource not found status code: 400, request id: ANCADFA6UC7RQSC76N396CH2GFVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:38:42 assoc store stderr: ResourceNotFoundException: Requested resource not found status code: 400, request id: 6AS3UUKMBH3RQ3EQPJ1FB6IO4JVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:38:42 assoc store stderr: RequestCanceled: request context canceled caused by: context canceled 2018/12/17 18:38:42 cache write flow 0936f2b1 state FlowDone {mem:8.0GiB cpu:8 disk:64.0GiB} exec image trinityctat/ctatfusion cmd " \n\t\ttar xvf %s -C %s\n\t" deps bf2ba2ec: ResourceNotFoundException: Requested resource not found status code: 400, request id: LJS0L7IG44S826QKI0G6UMHVT7VV4KQNSO5AEMVJF66Q9ASUAAJG ec2cluster: 2 instances: c5.9xlarge:2 (<=$3.1/hr), total{mem:133.9GiB cpu:72 disk:4.9TiB intel_avx:72 intel_avx2:72 intel_avx512:72}, waiting{}, pending{} transfers: e3de9706: elapsed: 11m, running:1, completed: 4/5 fusionDetectionReflow.StarFusion: exec trinityctat/ctatfusion ../STAR-Fusion --left_fq {{d}.. --denovo_reconstruct --CPU 8 51s 2018/12/17 18:39:34 ec2cluster: marking instance i-0e1873ad3b8883413 down: shutting-down 2018/12/17 18:39:34 ec2cluster: pending{} ec2cluster: 1 instances: c5.9xlarge:1 (<=$1.5/hr), total{mem:67.0GiB cpu:36 disk:2.4TiB intel_avx:36 intel_avx2:36 intel_avx512:36}, waiting{}, pending{} transfers: e3de9706: elapsed: 12m, running:1, completed: 4/5 fusionDetectionReflow.StarFusion: exec trinityctat/ctatfusion ../STAR-Fusion --left_fq {{d}.. --denovo_reconstruct --CPU 8 1m51s 2018/12/17 18:40:34 ec2cluster: pending{} ec2cluster: 1 instances: c5.9xlarge:1 (<=$1.5/hr), total{mem:67.0GiB cpu:36 disk:2.4TiB intel_avx:36 intel_avx2:36 intel_avx512:36}, waiting{}, pending{} transfers: e3de9706: elapsed: 13m, running:1, completed: 4/5 fusionDetectionReflow.StarFusion: exec trinityctat/ctatfusion ../STAR-Fusion --left_fq {{d}.. --denovo_reconstruct --CPU 8 2m51s 2018/12/17 18:41:33 <- fusionDetectionReflow.StarFusion 09d955ef ok exec 2m51s 18.2MiB 2018/12/17 18:41:33 fusionDetectionReflow.StarFusion 09d955ef /home/ubuntu/fusion_detection_pipeline/reflow/fusionDetectionReflow.rf:53:6: resources: {mem:64.0GiB cpu:8 disk:1000.0GiB} sha256:cf034633c130f3154feab2589aec06fa2835ce3ae28badb947e5d4326a33bbab sha256:09d955efb8ffc57b4253512697372bd5cad710973008ff2dad76e064d248188a ec2-52-24-239-24.us-west-2.compute.amazonaws.com:9000/9fff6e6847880d39/09d955efb8ffc57b4253512697372bd5cad710973008ff2dad76e064d248188a trinityctat/ctatfusion command: /usr/local/src/STAR-Fusion/STAR-Fusion --left_fq {{d}}/A10_1001000293_R1_001.fastq.gz --right_fq {{d}}/A10_1001000293_R2_001.fastq.gz --genome_lib_dir {{gBuildDecomp}}/GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ -O {{outDir}} --FusionInspector validate --examine_coding_effect --denovo_reconstruct --CPU 8 where: {{d}} = A10_1001000293_R1_001.fastq.gz sha256:7fe8583438a0925e2f308f318df37f384f3fe6925a3b515d4c986a1881a69b58 16.5KiB A10_1001000293_R2_001.fastq.gz sha256:07b7204847038de3e8c337ffffe3db06f270d29e18e59e93f2f1e048155e5104 16.7KiB {{d}} = A10_1001000293_R1_001.fastq.gz sha256:7fe8583438a0925e2f308f318df37f384f3fe6925a3b515d4c986a1881a69b58 16.5KiB A10_1001000293_R2_001.fastq.gz sha256:07b7204847038de3e8c337ffffe3db06f270d29e18e59e93f2f1e048155e5104 16.7KiB {{gBuildDecomp}} = GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/AnnotFilterRule.pm sha256:a57af06c37f03f1daf9f58fa4906990192eb303a3792130185fca3d6ffcef9cb 2.8KiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/blast_pairs.idx sha256:537cd37329c03079040c2df23229f438f29c8e4cf7c033410ccbad45001d5c0e 1.1GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/blast_pairs.idx.prev.1518210799 sha256:393503c7547092e3f5527a23dfec738ed298559c68c304132826152f8222e87d 1.1GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/fusion_annot_lib.idx sha256:76f709ad7373feac1127707113026f36d1c29d77b6026382769dd6e506f7ece0 2.2GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/pfam_domains.dbm sha256:ba8afc36d70491dedb53e8dbdf7b991b0433cd894824ea1061e024fcd9e429ab 191.7MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.cds sha256:65d7b76c1f0c9dc79aa582e32153263d2904a85f3290b58a71382ebb08cbaeab 105.4MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.gtf sha256:b74409636f142a951c1cbc578b45950fb299575ac00ce25c90bce85a26483f66 1.1GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.gtf.gene_spans sha256:45476e5c66d7a269fae32a77004e945345057ec85dd33c3a23bc8f31a3c77532 3.6MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.gtf.mini.sortu sha256:255a753ae422ea9e332df9ccafbe734420ab93add8b9b10e3860c90266bbf64b 47.8MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.pep sha256:0409e25d842f922cd408ed49f8983369e2877648b42059ad2ff58b91e22f6995 36.3MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_annot.prot_info.dbm sha256:62139d0941a7c2a586834520c5b8704810426a9a48a6c0a3c84a5706acf075ff 695.1MiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa sha256:7d517a1e1c1a05e0b5ee4af5da0aa11faecac7f2fbd89e98da627be399909f94 2.9GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.fai sha256:a3b9a0dc4fcdac8e1a8a66c23ac57c16e6195129f76742d313878c7c11686388 788B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.gmap.ok sha256:e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855 0B GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/Genome sha256:99790df229ca81fdecfdef3ad28dec074e87b7f160b2d01105a9f87b3b2764be 3.0GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SA sha256:f7bfab0be699f4bfee75680d2c8903831a23a479c8bf7a45b522e567f5b6aa94 22.8GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/SAindex sha256:80915c6510346ad234b2f6e1e1af8d0d2ce73822325462aaa85db18ceadb58e3 1.5GiB GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ref_genome.fa.star.idx/build.ok 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sha256:046eae7f012a47301a0993954569dac359627d9b18e545bacc3f5d1fcee7b825 227B star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.wSpliceInfo.wAnnot sha256:1a814f6fa77203cf7cf9ebe90c8e6b227836bde5b2e74d0d235a55e90de5f6c7 234B star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.wSpliceInfo.wAnnot.annot_filt sha256:a6ed881ff46894f65ecba6deb772c859f1e57e5e1feeaba1ab6dd1466d97d2e4 252B star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.wSpliceInfo.wAnnot.pass sha256:1a814f6fa77203cf7cf9ebe90c8e6b227836bde5b2e74d0d235a55e90de5f6c7 234B star-fusion.preliminary/star-fusion.fusion_candidates.preliminary.wSpliceInfo.wAnnot.pass.minFFPM.0.1.pass sha256:1a814f6fa77203cf7cf9ebe90c8e6b227836bde5b2e74d0d235a55e90de5f6c7 234B star-fusion.preliminary/star-fusion.junction_breakpts_to_genes.txt sha256:d0ab9cd5df6e360830ff132a2131a07ccb0940bf59aab3ecb04b080f9f15265e 5.3KiB star-fusion.preliminary/star-fusion.junction_breakpts_to_genes.txt.fail sha256:d00bbf261b45fe3985821eb6c18b97c0f25499755d35af93f15b6911435f66b3 5.6KiB star-fusion.preliminary/star-fusion.junction_breakpts_to_genes.txt.pass sha256:e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855 0B star-fusion.preliminary/star-fusion.junction_read_names sha256:e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855 0B star-fusion.preliminary/star-fusion.spanning_frag_names sha256:e3b0c44298fc1c149afbf4c8996fb92427ae41e4649b934ca495991b7852b855 0B profile: cpu mean=0.6 max=3.0 mem mean=25.0GiB max=34.8GiB disk mean=6.1MiB max=18.2MiB tmp mean=0B max=0B 2018/12/17 18:41:34 -> fusionDetectionReflow.Main 2bfbac31 run extern ..mmune/nonImmune_fastqs_9.27/170125/A10_1001000293/testOut/ 18.2MiB 2018/12/17 18:41:34 ec2cluster: pending{} 2018/12/17 18:41:35 assoc store execinspect: ResourceNotFoundException: Requested resource not found status code: 400, request id: P2INTI5D5ED9PQLNRG9RNCM9GNVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:41:35 assoc store execinspect: ResourceNotFoundException: Requested resource not found status code: 400, request id: SP1TTIA6VBROAIF4PVQO7T06QNVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:41:35 assoc store stdout: ResourceNotFoundException: Requested resource not found status code: 400, request id: 6LOPOQUOVSLFVDPAI7SK53RCMRVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:41:35 assoc store stdout: ResourceNotFoundException: Requested resource not found status code: 400, request id: PLHOMUDEUU6GDTJ2C3J5BFGB9NVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:41:35 assoc store stderr: ResourceNotFoundException: Requested resource not found status code: 400, request id: FJB1QG74UKIU4BVN8QOO70CH3BVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:41:35 assoc store stderr: RequestCanceled: request context canceled caused by: context canceled 2018/12/17 18:41:35 cache write flow 09d955ef state FlowDone {mem:64.0GiB cpu:8 disk:1000.0GiB} exec image trinityctat/ctatfusion cmd "\n\t\t/usr/local/src/STAR-Fusion/STAR-Fusion --left_fq %s/A10_1001000293_R1_001.fastq.gz --right_fq %s/A10_1001000293_R2_001.fastq.gz --genome_lib_dir %s/GRCh37_v19_CTAT_lib_Feb092018/ctat_genome_lib_build_dir/ -O %s --FusionInspector validate --examine_coding_effect --denovo_reconstruct --CPU 8\n\t" deps 2826e73f,2826e73f,0bd4d6cb: ResourceNotFoundException: Requested resource not found status code: 400, request id: ITSLEPVNP96U3C1C9FQ2EAO71JVV4KQNSO5AEMVJF66Q9ASUAAJG 2018/12/17 18:42:29 <- fusionDetectionReflow.Main 2bfbac31 err extern 51s 0B error exec sha256:2bfbac31899829e2cdabc7101ee79eaed8b1ad11397aa8e43b3de646def0c6c2: exited with code 1 /home/ubuntu/fusion_detection_pipeline/reflow/fusionDetectionReflow.rf:68:16 2018/12/17 18:42:29 total n=6 time=13m56s ident n ncache transfer runtime(m) cpu mem(GiB) disk(GiB) tmp(GiB) fusionDetectionReflow.Main 1 0 0B fusionDetectionReflow.StarFusion 1 0 0B 3/3/3 0.6/0.6/0.6 34.8/34.8/34.8 0.0/0.0/0.0 0.0/0.0/0.0 fusionDetectionReflow.Decompress 1 0 0B 4/4/4 1.1/1.1/1.1 0.1/0.1/0.1 33.2/33.2/33.2 0.0/0.0/0.0 fusionDetectionReflow.fq1 1 0 0B fusionDetectionReflow.fq2 1 0 0B fusionDetectionReflow.gBuildComp 1 0 0B 2018/12/17 18:42:29 marking run done after nonrecoverable error evaluation error: exec sha256:2bfbac31899829e2cdabc7101ee79eaed8b1ad11397aa8e43b3de646def0c6c2: exited with code 1 2018/12/17 18:42:29 run state: done error evaluation error: exec sha256:2bfbac31899829e2cdabc7101ee79eaed8b1ad11397aa8e43b3de646def0c6c2: exited with code 1 evaluation error: exec sha256:2bfbac31899829e2cdabc7101ee79eaed8b1ad11397aa8e43b3de646def0c6c2: exited with code 1