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Porting of samtools-ruby to BioRuby. Binder of samtools for ruby, on the top of FFI -from original project-

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README.rdoc

bio-samtools

The original project samtools-ruby belongs to Ricardo H. Ramirez @ github.com/homonecloco/samtools-ruby

Introduction

Documentation and code come from that project and we'll adapt it for a better integration in BioRuby.

Binder of samtools for ruby, on the top of FFI.

This project was born from the need to add support of BAM files to the gee_fu genome browser (github.com/danmaclean/gee_fu).

Installation

'gem install bio-samtools'

Usage

The easiest way to see bio-samtools in action is to try the tests rake basic_test rake pileup_test

See doc/tutorial.html or doc/tutorial.pdf for a walkthrough tutorial

Dependencies:

-FFI (github.com/ffi/ffi) -libbam This can be obtained from samtools. (samtools.sourceforge.net/ )

FAQ.

I´m getting a segmentation Fault, what did I do wrong? There are two known segmentation faults at the moment -When you try to load a text file as binary file -When you try to lad a binary file as a text file

If this is not the problem, or you have any other question, don´t hesitate on dropping a line to Ricardo dot Ramirez-Gonzalez at bbsrc dot ac dot uk dan.maclean@tsl.ac.uk

TODO

-Filter to the fetching algorithm (give a condition that has to be satisfied to add the alignment to the list) -Examples of how to use it, besides the test folder -Operating system independent, DONE ( test needed)

IMPORTANT NOTE

-Libraries are downloaded, compiled and installed inside the gem at install time on the host system -Library .a, I didn't check if it works on linux (test needed) $ openssl dgst libbam.so.1 MD5(libbam.so.1)= c45cfccfb41ffeb2730ee4b227d244c4

Contributing to bio-samtools

  • Check out the latest master to make sure the feature hasn't been implemented or the bug hasn't been fixed yet

  • Check out the issue tracker to make sure someone already hasn't requested it and/or contributed it

  • Fork the project

  • Start a feature/bugfix branch

  • Commit and push until you are happy with your contribution

  • Make sure to add tests for it. This is important so I don't break it in a future version unintentionally.

  • Please try not to mess with the Rakefile, version, or history. If you want to have your own version, or is otherwise necessary, that is fine, but please isolate to its own commit so I can cherry-pick around it.

Important Notes for developers

Remember that you must compile and install the right libbam library for you host system. In order to do that there are three possible solutions:

  • download, compile and install the library in bioruby-samtools-your_clone/lib/bio/db/sam/external/libbam.xxxxx by yourself

  • install the gem and then grab the compiled library cp `locate libbam.1.dylib` bioruby-samtools-your_clone/lib/bio/db/sam/external (library name is an example)

  • in your bioruby-samtools-your_clone create the Rakefile typing “cd ext; ruby mkrf_conf.rb; rake -f Rakefile”

The latest I think is the easiest way, cause you are replicating the automatic process.

For testing just run `rake test`. Tests must be improved.

Copyright

Copyright © 2011 Raoul J.P. Bonnal. See LICENSE.txt for further details.

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