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UNICS is a web app that helps you computing the standard error of allele frequency estimations of future pool-sequencing experiments based on different parameters (the number of individuals sampled, the frequency of the allele, the coefficient of variation of individual contributions to the DNA pool, the mean and dispersion in sequencing depth). You can use it trough the online version and read the related publication in press.

Here is a screenshot of the main sheets of the app: fig1

Local use

You can use the tool locally. You need R to be installed on your computer. Open R and use the code below:


Local installation

You can install the app on your machine.

1. Download the whole repository

2. Open R and make sure these library are installed:


If you miss a library, remember you can install it with:


3. Then, set the working directory and run the App:



If you find this application useful, please cite:
How to optimize the precision of allele and haplotype frequency estimates using pooled-sequencing data
Nicolas O. Rode, Yan Holtz, Karine Loridon, Sylvain Santoni, Joëlle Ronfort & Laurène Gay
INRA, UMR AGAP, F-34060 Montpellier, France Current address: INRA, UMR CBGP (INRA – IRD – Cirad – Montpellier SupAgro), Campus international de Baillarguet, CS 30016, F-34988, Montferrier-sur-Lez, France

About us

Nicolas O. Rode: homepage
Yan Holtz: homepage
Karine Loridon: contact
Sylvain Santoni: contact
Joëlle Ronfort: contact
Laurène Gay: homepage

We would like to thank Cédric Goby for his help during the shiny server deployment.