diff --git a/deno_dist/middleware.ts b/deno_dist/middleware.ts
index 5b421668b..6164d61a4 100644
--- a/deno_dist/middleware.ts
+++ b/deno_dist/middleware.ts
@@ -13,6 +13,7 @@ export { jwt } from './middleware/jwt/index.ts'
 export * from './middleware/logger/index.ts'
 export * from './middleware/method-override/index.ts'
 export * from './middleware/powered-by/index.ts'
+export * from './middleware/timeout/index.ts'
 export * from './middleware/timing/index.ts'
 export * from './middleware/pretty-json/index.ts'
 export * from './middleware/secure-headers/index.ts'
diff --git a/deno_dist/middleware/timeout/index.ts b/deno_dist/middleware/timeout/index.ts
new file mode 100644
index 000000000..076bd80aa
--- /dev/null
+++ b/deno_dist/middleware/timeout/index.ts
@@ -0,0 +1,53 @@
+import type { Context } from '../../context.ts'
+import { HTTPException } from '../../http-exception.ts'
+import type { MiddlewareHandler } from '../../types.ts'
+
+export type HTTPExceptionFunction = (context: Context) => HTTPException
+
+const defaultTimeoutException = new HTTPException(504, {
+  message: 'Gateway Timeout',
+})
+
+/**
+ * Timeout middleware for Hono.
+ *
+ * @param {number} duration - The timeout duration in milliseconds.
+ * @param {HTTPExceptionFunction | HTTPException} [exception=defaultTimeoutException] - The exception to throw when the timeout occurs. Can be a function that returns an HTTPException or an HTTPException object.
+ * @returns {MiddlewareHandler} The middleware handler function.
+ *
+ * @example
+ * ```ts
+ * const app = new Hono()
+ *
+ * app.use(
+ *   '/long-request',
+ *   timeout(5000) // Set timeout to 5 seconds
+ * )
+ *
+ * app.get('/long-request', async (c) => {
+ *   await someLongRunningFunction()
+ *   return c.text('Completed within time limit')
+ * })
+ * ```
+ */
+export const timeout = (
+  duration: number,
+  exception: HTTPExceptionFunction | HTTPException = defaultTimeoutException
+): MiddlewareHandler => {
+  return async function timeout(context, next) {
+    let timer: number | undefined
+    const timeoutPromise = new Promise<void>((_, reject) => {
+      timer = setTimeout(() => {
+        reject(typeof exception === 'function' ? exception(context) : exception)
+      }, duration) as unknown as number
+    })
+
+    try {
+      await Promise.race([next(), timeoutPromise])
+    } finally {
+      if (timer !== undefined) {
+        clearTimeout(timer)
+      }
+    }
+  }
+}
diff --git a/package.json b/package.json
index 043957ff6..7deaa52ed 100644
--- a/package.json
+++ b/package.json
@@ -179,6 +179,11 @@
       "import": "./dist/middleware/jwt/index.js",
       "require": "./dist/cjs/middleware/jwt/index.js"
     },
+    "./timeout": {
+      "types": "./dist/types/middleware/timeout/index.d.ts",
+      "import": "./dist/middleware/timeout/index.js",
+      "require": "./dist/cjs/middleware/timeout/index.js"
+    },
     "./timing": {
       "types": "./dist/types/middleware/timing/index.d.ts",
       "import": "./dist/middleware/timing/index.js",
@@ -420,6 +425,9 @@
       "jwt": [
         "./dist/types/middleware/jwt"
       ],
+      "timeout": [
+        "./dist/types/middleware/timeout"
+      ],
       "timing": [
         "./dist/types/middleware/timing"
       ],
diff --git a/src/middleware.ts b/src/middleware.ts
index 65954a4ed..c6c7a4c72 100644
--- a/src/middleware.ts
+++ b/src/middleware.ts
@@ -13,6 +13,7 @@ export { jwt } from './middleware/jwt'
 export * from './middleware/logger'
 export * from './middleware/method-override'
 export * from './middleware/powered-by'
+export * from './middleware/timeout'
 export * from './middleware/timing'
 export * from './middleware/pretty-json'
 export * from './middleware/secure-headers'
diff --git a/src/middleware/timeout/index.test.ts b/src/middleware/timeout/index.test.ts
new file mode 100644
index 000000000..6d24a5257
--- /dev/null
+++ b/src/middleware/timeout/index.test.ts
@@ -0,0 +1,70 @@
+import type { Context } from '../../context'
+import { Hono } from '../../hono'
+import { HTTPException } from '../../http-exception'
+import type { HTTPExceptionFunction } from '.'
+import { timeout } from '.'
+
+describe('Timeout API', () => {
+  const app = new Hono()
+
+  app.use('/slow-endpoint', timeout(1000))
+  app.use(
+    '/slow-endpoint/custom',
+    timeout(
+      1100,
+      () => new HTTPException(408, { message: 'Request timeout. Please try again later.' })
+    )
+  )
+  const exception500: HTTPExceptionFunction = (context: Context) =>
+    new HTTPException(500, { message: `Internal Server Error at ${context.req.path}` })
+  app.use('/slow-endpoint/error', timeout(1200, exception500))
+  app.use('/normal-endpoint', timeout(1000))
+
+  app.get('/slow-endpoint', async (c) => {
+    await new Promise((resolve) => setTimeout(resolve, 1100))
+    return c.text('This should not show up')
+  })
+
+  app.get('/slow-endpoint/custom', async (c) => {
+    await new Promise((resolve) => setTimeout(resolve, 1200))
+    return c.text('This should not show up')
+  })
+
+  app.get('/slow-endpoint/error', async (c) => {
+    await new Promise((resolve) => setTimeout(resolve, 1300))
+    return c.text('This should not show up')
+  })
+
+  app.get('/normal-endpoint', async (c) => {
+    await new Promise((resolve) => setTimeout(resolve, 900))
+    return c.text('This should not show up')
+  })
+
+  it('Should trigger default timeout exception', async () => {
+    const res = await app.request('http://localhost/slow-endpoint')
+    expect(res).not.toBeNull()
+    expect(res.status).toBe(504)
+    expect(await res.text()).toContain('Gateway Timeout')
+  })
+
+  it('Should apply custom exception with function', async () => {
+    const res = await app.request('http://localhost/slow-endpoint/custom')
+    expect(res).not.toBeNull()
+    expect(res.status).toBe(408)
+    expect(await res.text()).toContain('Request timeout. Please try again later.')
+  })
+
+  it('Error timeout with custom status code and message', async () => {
+    const res = await app.request('http://localhost/slow-endpoint/error')
+    expect(res).not.toBeNull()
+    expect(res.status).toBe(500)
+    expect(await res.text()).toContain('Internal Server Error at /slow-endpoint/error')
+  })
+
+  it('No Timeout should pass', async () => {
+    const res = await app.request('http://localhost/normal-endpoint')
+    expect(res).not.toBeNull()
+    expect(res.status).toBe(200)
+    expect(await res.text()).toContain('This should not show up')
+  })
+})
diff --git a/src/middleware/timeout/index.ts b/src/middleware/timeout/index.ts
new file mode 100644
index 000000000..e7102e18b
--- /dev/null
+++ b/src/middleware/timeout/index.ts
@@ -0,0 +1,53 @@
+import type { Context } from '../../context'
+import { HTTPException } from '../../http-exception'
+import type { MiddlewareHandler } from '../../types'
+
+export type HTTPExceptionFunction = (context: Context) => HTTPException
+
+const defaultTimeoutException = new HTTPException(504, {
+  message: 'Gateway Timeout',
+})
+
+/**
+ * Timeout middleware for Hono.
+ *
+ * @param {number} duration - The timeout duration in milliseconds.
+ * @param {HTTPExceptionFunction | HTTPException} [exception=defaultTimeoutException] - The exception to throw when the timeout occurs. Can be a function that returns an HTTPException or an HTTPException object.
+ * @returns {MiddlewareHandler} The middleware handler function.
+ *
+ * @example
+ * ```ts
+ * const app = new Hono()
+ *
+ * app.use(
+ *   '/long-request',
+ *   timeout(5000) // Set timeout to 5 seconds
+ * )
+ *
+ * app.get('/long-request', async (c) => {
+ *   await someLongRunningFunction()
+ *   return c.text('Completed within time limit')
+ * })
+ * ```
+ */
+export const timeout = (
+  duration: number,
+  exception: HTTPExceptionFunction | HTTPException = defaultTimeoutException
+): MiddlewareHandler => {
+  return async function timeout(context, next) {
+    let timer: number | undefined
+    const timeoutPromise = new Promise<void>((_, reject) => {
+      timer = setTimeout(() => {
+        reject(typeof exception === 'function' ? exception(context) : exception)
+      }, duration) as unknown as number
+    })
+
+    try {
+      await Promise.race([next(), timeoutPromise])
+    } finally {
+      if (timer !== undefined) {
+        clearTimeout(timer)
+      }
+    }
+  }
+}