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---- README ------ THE FOLLOWING TWO FILES MUST BE PLACED IN THE MATLAB JAVA DIRECTORY C:\Matlab\Java... 1. ij.jar 2. mij.jar THE FOLLOWING TWO FILES MUST BE PLACED IN THE IMAGEJ PLUGINS DIRECTORY C:\Program Files\ImageJ\plugins\Analyze 1. Nano_TrackingBis_orig.java 2. Nano_TrackingBis.java --NOTES-- 1. Memory limit in matlab imageJ: http://imagej.1557.x6.nabble.com/increasing-memory-allocation-via-Java-code-td3689982.html 2. Save tif stack in matlab failure: http://blogs.mathworks.com/steve/2010/11/09/the-mystery-of-the-failing-tiff-append-loop/ Error using imwrite (line 455) Unable to open file "C:\Users\Nicolas\Desktop\ONLY_BACTRACK_DATA\test2\WT30sThree_BW.tif" for writing. You may not have write permission. tldr; Make sure all windows explorer windows are closed!