diff --git a/test/test_source_osv.py b/test/test_source_osv.py
index 90cba79889..716023b959 100644
--- a/test/test_source_osv.py
+++ b/test/test_source_osv.py
@@ -11,6 +11,7 @@
 
 import aiohttp
 import pytest
+import requests
 
 from cve_bin_tool.data_sources import osv_source
 
@@ -27,36 +28,7 @@ def teardown_class(cls):
         shutil.rmtree(cls.osv.cachedir)
 
     osv_url = "https://osv-vulnerabilities.storage.googleapis.com/"
-
-    ecosystems = [
-        "Android",
-        "DWF",
-        "Debian",
-        "Debian:10",
-        "Debian:11",
-        "Debian:3.0",
-        "Debian:3.1",
-        "Debian:4.0",
-        "Debian:5.0",
-        "Debian:6.0",
-        "Debian:7",
-        "Debian:8",
-        "Debian:9",
-        "GSD",
-        "Go",
-        "Hex",
-        "JavaScript",
-        "Linux",
-        "Maven",
-        "NuGet",
-        "OSS-Fuzz",
-        "Packagist",
-        "PyPI",
-        "RubyGems",
-        "UVI",
-        "crates.io",
-        "npm",
-    ]
+    ecosystems_url = "https://osv-vulnerabilities.storage.googleapis.com/ecosystems.txt"
 
     zip_namelist = [
         "GSD-2021-1000000.json",
@@ -198,7 +170,18 @@ def teardown_class(cls):
     async def test_update_ecosystems(self):
         await self.osv.update_ecosystems()
 
-        assert all(x in self.osv.ecosystems for x in self.ecosystems)
+        ecosystems_txt = requests.get(self.ecosystems_url, timeout=300).text.strip("\n")
+        expected_ecosystems = set(ecosystems_txt.split("\n"))
+
+        # Because ecosystems.txt does not contain the complete list, this must be
+        # manually fixed up.
+        expected_ecosystems.add("DWF")
+        expected_ecosystems.add("JavaScript")
+
+        # Assert that there are no missing ecosystems
+        assert all(x in self.osv.ecosystems for x in expected_ecosystems)
+        # Assert that there are no extra ecosystems
+        assert all(x in expected_ecosystems for x in self.osv.ecosystems)
 
     @pytest.mark.asyncio
     @pytest.mark.skipif(not EXTERNAL_SYSTEM(), reason="Needs network connection.")