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CODEX Single Cell Multiplexed Imaging Dashboard

Binder

citeseq_adt

Goltsev et al used a highly multiplexed cytometric approach called CODEX to measure ~30 surface markers in spatially resolved single cells from mouse spleens. We utilized Clustergrammer2 to hierarchically cluster ~5,000 sinlge cells (from a subset of a segmented spleen image). We also used the Jupyter Widget bqplot to visualize single cell location data using voronoi plots. We then built a dasnboard using the library voila, which converts Jupyter notebooks to dashboards/web-apps, and linked our heatmap to the spatial map. This allows to interact with the Clustergrammer2 heatmap and highlight cells in the spatially resolved map. These kind of linked views are crucial for exploration of spatially resolved high-dimensional single cell data. Finally, we are running this dashboard using MyBinder.

YouTube Tutorial

10K PBMC CITE-seq

CODEX data from: http://welikesharingdata.blob.core.windows.net/forshare/index.html

Voila dashboard example originally from: https://github.com/voila-gallery/gaussian-density

Note: works best in Chrome Browser.

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Single Cell Multiplexed Imaging Jupyter Voila Dashboard using CODEX Data

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