After updating my cyberduck to 4.4(13577) on my 2 Windows machines (Win 7 & Win 8), the permission tab on my amazon connection is disabled.
To replicate the error, open a Amazon S3 connection. Right click any file and choose info. the permissions tab is greyed out.
the only way i can temporarily bypass this, is to connect to a non-amazon s3 connection, right click a file and open the permissions tab. then switch connection to my amazon s3, so i can see the permissions tab (but the button is still disabled)