diff --git a/debug_toolbar/panels/staticfiles.py b/debug_toolbar/panels/staticfiles.py
index 5f9efb5c3..2eed2efa0 100644
--- a/debug_toolbar/panels/staticfiles.py
+++ b/debug_toolbar/panels/staticfiles.py
@@ -4,7 +4,6 @@
 
 from django.conf import settings
 from django.contrib.staticfiles import finders, storage
-from django.core.checks import Warning
 from django.utils.functional import LazyObject
 from django.utils.translation import gettext_lazy as _, ngettext
 
@@ -178,27 +177,3 @@ def get_staticfiles_apps(self):
                     if app not in apps:
                         apps.append(app)
         return apps
-
-    @classmethod
-    def run_checks(cls):
-        """
-        Check that the integration is configured correctly for the panel.
-
-        Specifically look for static files that haven't been collected yet.
-
-        Return a list of :class: `django.core.checks.CheckMessage` instances.
-        """
-        errors = []
-        for finder in finders.get_finders():
-            try:
-                for path, finder_storage in finder.list([]):
-                    finder_storage.path(path)
-            except OSError:
-                errors.append(
-                    Warning(
-                        "debug_toolbar requires the STATICFILES_DIRS directories to exist.",
-                        hint="Running manage.py collectstatic may help uncover the issue.",
-                        id="debug_toolbar.staticfiles.W001",
-                    )
-                )
-        return errors
diff --git a/docs/changes.rst b/docs/changes.rst
index fd79230af..5e6fbb24d 100644
--- a/docs/changes.rst
+++ b/docs/changes.rst
@@ -13,6 +13,10 @@ Pending
 * Stayed on top of pre-commit hook updates.
 * Added :doc:`architecture documentation <architecture>` to help
   on-board new contributors.
+* Removed the static file path validation check in
+  :class:`StaticFilesPanel <debug_toolbar.panels.staticfiles.StaticFilesPanel>`
+  since that check is made redundant by a similar check in Django 4.0 and
+  later.
 
 4.3.0 (2024-02-01)
 ------------------
diff --git a/tests/panels/test_staticfiles.py b/tests/panels/test_staticfiles.py
index 32ed7ea61..0736d86ed 100644
--- a/tests/panels/test_staticfiles.py
+++ b/tests/panels/test_staticfiles.py
@@ -1,15 +1,8 @@
-import os
-import unittest
-
-import django
 from django.conf import settings
 from django.contrib.staticfiles import finders
-from django.test.utils import override_settings
 
 from ..base import BaseTestCase
 
-PATH_DOES_NOT_EXIST = os.path.join(settings.BASE_DIR, "tests", "invalid_static")
-
 
 class StaticFilesPanelTestCase(BaseTestCase):
     panel_id = "StaticFilesPanel"
@@ -52,33 +45,3 @@ def test_insert_content(self):
             "django.contrib.staticfiles.finders.AppDirectoriesFinder", content
         )
         self.assertValidHTML(content)
-
-    @unittest.skipIf(django.VERSION >= (4,), "Django>=4 handles missing dirs itself.")
-    @override_settings(
-        STATICFILES_DIRS=[PATH_DOES_NOT_EXIST] + settings.STATICFILES_DIRS,
-        STATIC_ROOT=PATH_DOES_NOT_EXIST,
-    )
-    def test_finder_directory_does_not_exist(self):
-        """Misconfigure the static files settings and verify the toolbar runs.
-
-        The test case is that the STATIC_ROOT is in STATICFILES_DIRS and that
-        the directory of STATIC_ROOT does not exist.
-        """
-        response = self.panel.process_request(self.request)
-        self.panel.generate_stats(self.request, response)
-        content = self.panel.content
-        self.assertIn(
-            "django.contrib.staticfiles.finders.AppDirectoriesFinder", content
-        )
-        self.assertNotIn(
-            "django.contrib.staticfiles.finders.FileSystemFinder (2 files)", content
-        )
-        self.assertEqual(self.panel.num_used, 0)
-        self.assertNotEqual(self.panel.num_found, 0)
-        expected_apps = ["django.contrib.admin", "debug_toolbar"]
-        if settings.USE_GIS:
-            expected_apps = ["django.contrib.gis"] + expected_apps
-        self.assertEqual(self.panel.get_staticfiles_apps(), expected_apps)
-        self.assertEqual(
-            self.panel.get_staticfiles_dirs(), finders.FileSystemFinder().locations
-        )
diff --git a/tests/test_checks.py b/tests/test_checks.py
index 8e4f8e62f..b88787f9d 100644
--- a/tests/test_checks.py
+++ b/tests/test_checks.py
@@ -1,14 +1,8 @@
-import os
-import unittest
 from unittest.mock import patch
 
-import django
-from django.conf import settings
 from django.core.checks import Warning, run_checks
 from django.test import SimpleTestCase, override_settings
 
-PATH_DOES_NOT_EXIST = os.path.join(settings.BASE_DIR, "tests", "invalid_static")
-
 
 class ChecksTestCase(SimpleTestCase):
     @override_settings(
@@ -92,23 +86,6 @@ def test_check_middleware_classes_error(self):
             messages,
         )
 
-    @unittest.skipIf(django.VERSION >= (4,), "Django>=4 handles missing dirs itself.")
-    @override_settings(
-        STATICFILES_DIRS=[PATH_DOES_NOT_EXIST],
-    )
-    def test_panel_check_errors(self):
-        messages = run_checks()
-        self.assertEqual(
-            messages,
-            [
-                Warning(
-                    "debug_toolbar requires the STATICFILES_DIRS directories to exist.",
-                    hint="Running manage.py collectstatic may help uncover the issue.",
-                    id="debug_toolbar.staticfiles.W001",
-                )
-            ],
-        )
-
     @override_settings(DEBUG_TOOLBAR_PANELS=[])
     def test_panels_is_empty(self):
         errors = run_checks()