Welcome to SnakeLines
With decreasing price of massive parallel sequencing technologies, more and more laboratories are utilizing resulting sequences of DNA fragments for genomic analysis. An substantial obstacle for interpretation is transforming of sequenced data into results interpretable by clinicians and researchers without computational background. Laboratories are generally using computational pipelines consisting of several bioinformatic tools.
We propose several computational pipelines for processing of paired-end Illumina reads; including mapping, assembly, variant calling, viral identification, RNA-seq and metagenomics analysis. All provided pipelines are embedded into virtual environments that ensures isolation of required resources from host operating system, rapid deployment and reproducibilty of results accross different platforms.
How to execute pipelines
Source code of the SnakeLines pipelines can be downloaded from the Github repository. The documentation is accessible from the ReadTheDocs. See the Quick start or the Running SnakeLines section of the documentation to see instructions for execution of pipelines.
Why use SnakeLines
SnakeLines contains wide set of computational pipelines that are ready to use immediately after downloading SnakeLines sources. In addition to standard secondary analysis, such as mapping and assembly, SnakeMake facilitate:
- identification of variation in mapped reads
- metagenomics analysis
- methylation profiles
Each step of analysis is supported by graphical or tabular reports. Essential output and report files are stored in separate, report directory. In addition to reports that are generated for each sample individually, SnakeLines store also aggregated reports to examine quality of several samples at once.
No need to install additional tools
SnakeLines requires only Snakemake and Miniconda to be installed. The rest of bioinformatic tools required for analysis are compiled automatically. Tools are installed into separated virtual environments. This way, they do not break any installation or dependencies on the operating system.
Each pipeline is parametrized in a single, YAML based configuration file. Configuration is useful overview over analysis, since every step of the analysis is configured there in sequential order. You may easily swap tools or change their parameters by adjusting the configuration file.
Structure of the SnakeLines sources allows to easily extend existing pipelines, or just replace method in some step with custom solution.