diff --git a/CHANGELOG.md b/CHANGELOG.md
index 85c66a5d976f..f881d9a48448 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -22,6 +22,8 @@
 
 ### Performance
 
+- `[jest-core]` Cache micromatch in SearchSource globsToMatcher
+
 ## 26.0.1
 
 ### Fixes
diff --git a/packages/jest-core/src/SearchSource.ts b/packages/jest-core/src/SearchSource.ts
index 48c630f2adb4..763e868eba14 100644
--- a/packages/jest-core/src/SearchSource.ts
+++ b/packages/jest-core/src/SearchSource.ts
@@ -37,8 +37,57 @@ export type TestSelectionConfig = {
   watch?: boolean;
 };
 
-const globsToMatcher = (globs: Array<Config.Glob>) => (path: Config.Path) =>
-  micromatch([replacePathSepForGlob(path)], globs, {dot: true}).length > 0;
+const globsMatchers = new Map<
+  string,
+  {
+    isMatch: (str: string) => boolean;
+    negated: boolean;
+  }
+>();
+
+const globsToMatcher = (globs: Array<Config.Glob>) => {
+  const matchers = globs.map(glob => {
+    if (!globsMatchers.has(glob)) {
+      const state = micromatch.scan(glob, {dot: true});
+      const matcher = {
+        isMatch: micromatch.matcher(glob, {dot: true}),
+        negated: state.negated,
+      };
+      globsMatchers.set(glob, matcher);
+    }
+    return globsMatchers.get(glob);
+  });
+
+  return (path: Config.Path) => {
+    const omit = new Set();
+    const keep = new Set();
+    let negatives = 0;
+    const replacedPath = replacePathSepForGlob(path);
+
+    for (let i = 0; i < matchers.length; i++) {
+      const matcher = matchers[i];
+      if (!matcher) continue;
+      const {isMatch, negated} = matcher;
+      if (negated) negatives++;
+
+      const matched = isMatch(replacedPath);
+
+      const match = negated ? !matched : matched;
+      if (!match) continue;
+
+      if (negated) {
+        omit.add(replacedPath);
+      } else {
+        omit.delete(replacedPath);
+        keep.add(replacedPath);
+      }
+    }
+
+    const result = negatives === matchers.length ? [replacedPath] : [...keep];
+
+    return result.some(item => !omit.has(item));
+  };
+};
 
 const regexToMatcher = (testRegex: Config.ProjectConfig['testRegex']) => (
   path: Config.Path,